CAZyme Information: Afu5g10530-T-p1

Basic Information

• Species: Aspergillus fumigatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus fumigatus
• CAZyme ID: Afu5g10530-T-p1
• CAZy Family: GH5
• CAZyme Description: Rhamnogalacturonase A, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
521		54976.62	6.6540

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AfumigatusAf293	10130	330879	290	9840

• Gene Location: location=Chr5_A_fumigatus_Af293:2694101-2695858(+)

Full Sequence      

MRLHAFTLLSLLGLVPSFAAASLSGSVGPLTSASAKAAKKTCNVLDYGAKADKKTDLGPP
LAAAFAACKSGGLVYIPAGDYAMSTWVKLANGKAWALQIDGVIYRTGTDGGNMIMIEHTS
DFELYSSTSSGAMQGLGYEFHASNNWSGPRLLRLWDVSDFSVHDLILVDSPSFHFSIDTC
SNGEVYNMAIRGGNHGGLDGVDVWSTNIWIHDLEVTNKDECVTVKSPAKNILVENIYCNL
SGGCAMGSLGADTDISDITYKNIYTWNSNQMMMIKSNGGSGTVSNVVFENFIGHGNAYSL
DIDSFWSSMSAVSGDGVTLNNITIKNWKGTEANGAQRGPIKIICPDKVPCYNILIEDFAM
WTETGSKQWYSCQSAYGSGFCLKSGSHHTSYAVTTTTVSSAPSGYSAAKMASDLSTDSGS
TKSIPIPTIPTSFYPGATPYSALMSKQSTKAAKARAVDMSVETPAAASRSEQVVQGAPQE
TGQSAPESAGPVPSGNPGPVPTGGSRPSRHRHGHHHFGSAI

Enzyme Prediction     

EC	3.2.1.171:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	66	375	1.1e-55	0.96

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395231	Glyco_hydro_28	2.68e-25	142	373	75	313	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
215540	PLN03010	5.50e-20	43	377	48	391	polygalacturonase
177865	PLN02218	7.51e-19	38	356	64	404	polygalacturonase ADPG
215426	PLN02793	1.17e-16	130	378	150	412	Probable polygalacturonase
215120	PLN02188	1.26e-15	5	375	2	390	polygalacturonase/glycoside hydrolase family protein

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE66335.1|GH28	1.10e-270	1	475	1	477
UDD65679.1|GH28	1.10e-270	1	475	1	477
QRD92579.1|GH28	6.36e-270	1	475	1	477
CAK48512.1|GH28	6.33e-267	1	452	1	452
CAA63912.1|GH28|3.2.1.171	2.57e-266	1	452	1	452

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RMG_A	3.98e-228	23	443	2	420	Rhamnogalacturonase A From Aspergillus Aculeatus [Aspergillus aculeatus]
1HG8_A	1.34e-10	199	336	167	310	Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme [Fusarium verticillioides]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A1DED1|RHGB_NEOFI	0.0	1	517	1	512	Probable rhamnogalacturonase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=rhgB PE=3 SV=1
sp|Q4WV23|RHGB_ASPFU	0.0	1	521	1	521	Probable rhamnogalacturonase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=rhgB PE=3 SV=1
sp|B0Y0Q3|RHGB_ASPFC	0.0	1	521	1	521	Probable rhamnogalacturonase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=rhgB PE=3 SV=1
sp|Q2TWN8|RHGB_ASPOR	1.96e-271	1	475	1	477	Probable rhamnogalacturonase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rhgB PE=3 SV=1
sp|Q0CFC7|RHGB_ASPTN	2.12e-269	1	482	1	477	Probable rhamnogalacturonase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=rhgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000287	0.999718	CS pos: 19-20. Pr: 0.9807

TMHMM  Annotations     

There is no transmembrane helices in Afu5g10530-T-p1.