CAZyme Information: ATY62742.1

Basic Information

• Species: Cordyceps militaris
• Lineage: Ascomycota; Sordariomycetes; ; Cordycipitaceae; Cordyceps; Cordyceps militaris
• CAZyme ID: ATY62742.1
• CAZy Family: GH25
• CAZyme Description: Glycoside family 47

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1095	CP023324|CGC33	120593.33	6.1665

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_CmilitarisATCC34164	9362	N/A	75	9287

• Gene Location: location=CP023324:1961163-1964511(-)

Full Sequence      

MFLLATLFALSALPWTAPAMRSDHLAKLRQETVDMFYHGYNNYMLHAFPEDELRPVSCQP
LTRNPNKPADIGLNDVLGNYSLTLIDSLSTLAILAGGPANEKYTGSQALSDFQDGVAQFV
SYYGDGRQGSSGQGLRSRGFDLDSKVQVFETVIRGVGGLLSAHLFAVGDLPIRGYDPKPD
SKWASEDPLETAPISWHGGFRYDGQLLRLALDLSERLLPAFYTKTGIPYPRVNLRSGIPF
YINSPLHQDSEDTEPVVGNAEITETCSAGAGSLTLEFTVLSRLSGDPRFEQAAKRAFWEV
WERRSDIGLIGNGIDAERGVWIGPHSGIGAGMDSFFEYALKSHILLSGQETPNMTRSHRQ
STTGWLDPNSLHPPLPPEMHSSDAFLEAWHQAHASVKRYIYTDRNHFPYYSNNHRATGQP
YTMWIDSLGAFYPGLLALAGEVDEAVEANLVYTALWTRYGAIPERWSVRESNVEPGIAWW
PGRPEFIESTYHIYRATADPWYLRVGEMVLHDIQRRCWVACGWAGLENVLSGEKQDRMES
FFLGETTKYLYLLFDPEHPLNNLDAAYVFTTEGHPLIIPRRARGSSTPRRRASRTSRQEE
KDVAVYSYYDTRFTNSCPAARTPTEPLAGSNTAARRNLFDVSRFTNLYNTPNIHGPLESF
KTRDEHKGVITQYRATSNYSVFPWTLPPTMLPHNGTCPVPSNRILSWIEFPTVDPAPSLF
ATRGGGGDSNTPLRWYHNVGPTVERVDGLKLQLEQEFNEAAGTDVWMVTHVAGKTVGRHE
NVIFHADHVRHFRDDAFSVLRRKDMVEVVLLVQPEPTPQASRELPPAVMPLPVPGALDEV
SSLDAAPDNTSSTRRPSVRASGQPPATDPDSLFKSILRAVSSALEGSATASTPSPSPLRG
SGSGSGSRDDSSNSDDDVGVASIHSWLANTATGAGAFPLPSVRDALLRNSPDFSAADPAA
SLPWRHVYMAGSACDGPLPEDAPRRHQVIVMRRGGCPFGDKLAHVPNFVPDEASLQLVVV
VDEEGGGHVDDAPRPLLATEQRTPRGTPRLHGIPMVLLPASKGTYELFGEAAAVGLRRKY
VVKSQGYVIENAIVM

Enzyme Prediction     

No EC number prediction in ATY62742.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH47	35	577	1.6e-140	0.9955156950672646

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396217	Glyco_hydro_47	1.07e-132	35	578	1	453	Glycosyl hydrolase family 47. Members of this family are alpha-mannosidases that catalyze the hydrolysis of the terminal 1,2-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man(9)(GlcNAc)(2).
240427	PTZ00470	6.31e-59	22	579	65	519	glycoside hydrolase family 47 protein; Provisional
239039	PA_PoS1_like	0.002	973	1004	43	74	PA_PoS1_like: Protease-associated (PA) domain PoS1-like. This group includes various PA domain-containing proteins similar to Pleurotus ostreatus (Po)S1. PoSl, the main extracellular protease in P. ostreatus is a subtilisin-like serine protease belonging to the peptidase S8 family. Ca2+ and Mn2+ both stimulate the protease activity of (Po)S1. Ca2+ protects PoS1 from autolysis. PoS1 is a monomeric glycoprotein, which may play a role in the regulation of laccases in lignin formation. (Po)S1 participates in the degradation of POXA1b, and in the activation of POXA3, (POXA1b and POXA3 are laccase isoenzymes), but its effect may be indirect. The significance of the PA domain to PoS1 has not been ascertained. It may be a protein-protein interaction domain. At peptidase active sites, the PA domain may participate in substrate binding and/or promoting conformational changes, which influence the stability and accessibility of the site to substrate.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATY62742.1|GH47	0.0	1	1095	1	1095
QLI71571.1|GH47	0.0	1	1095	1	1056
UKZ90649.1|GH47	0.0	19	1095	21	1063
UKZ65191.1|GH47	0.0	19	1095	21	1057
QYS97035.1|GH47	0.0	3	1095	5	1064

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5KIJ_A	8.33e-35	24	578	1	451	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase and Man9GlcNAc2-PA complex [Homo sapiens]
1FMI_A	9.20e-35	24	578	6	456	Crystal Structure Of Human Class I Alpha1,2-Mannosidase [Homo sapiens]
1FO2_A	9.50e-35	24	578	6	456	Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With 1-Deoxymannojirimycin [Homo sapiens],1FO3_A Crystal Structure Of Human Class I Alpha1,2-Mannosidase In Complex With Kifunensine [Homo sapiens]
1X9D_A	2.90e-34	24	578	84	534	Crystal Structure Of Human Class I alpha-1,2-Mannosidase In Complex With Thio-Disaccharide Substrate Analogue [Homo sapiens]
5KK7_A	3.88e-34	24	578	1	451	Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex [Homo sapiens],5KK7_B Crystal structure of the class I human endoplasmic reticulum 1,2-alpha-mannosidase T688A mutant and Thio-disaccharide substrate analog complex [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O94726|MNL1_SCHPO	1.32e-120	19	589	26	488	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mnl1 PE=3 SV=2
sp|Q925U4|EDEM1_MOUSE	2.09e-116	26	583	123	586	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Mus musculus OX=10090 GN=Edem1 PE=1 SV=1
sp|Q92611|EDEM1_HUMAN	4.93e-114	26	578	128	586	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Homo sapiens OX=9606 GN=EDEM1 PE=1 SV=1
sp|P38888|MNL1_YEAST	1.34e-105	17	585	24	509	ER degradation-enhancing alpha-mannosidase-like protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MNL1 PE=1 SV=1
sp|Q9SXC9|MNS5_ARATH	1.04e-90	27	578	36	478	Alpha-mannosidase I MNS5 OS=Arabidopsis thaliana OX=3702 GN=MNS5 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000262	0.999715	CS pos: 19-20. Pr: 0.9648

TMHMM  Annotations     

There is no transmembrane helices in ATY62742.1.