CAZyme Information: ATEG_10158-t26_1-p1

Basic Information

• Species: Aspergillus terreus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus terreus
• CAZyme ID: ATEG_10158-t26_1-p1
• CAZy Family: GT4
• CAZyme Description: conserved hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1270	CH476609|CGC9	136270.17	4.5825

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AterreusNIH2624	10551	341663	150	10401

• Gene Location: location=CH476609:577231-581099(-)

Full Sequence      

MRLSFVLLGSLLGLVSAVTDSYNGQYADDCPSLCADAGPSPANWTNIHHLRNLESCDQTV
LFGFNIQNSVADPNTILTIRACVASPGQTYEAAASPDVPQQQSQPNLVVAESCGAKAVKT
AFIPQVGPVTLSKASGAAPQSAHVAEAARQLALFVDRSAECGTTILFAKHESAVVGLYAG
AQITKHAARNLVDSFAEKQTSALQICQPASAALTVGVVSAGFIDLAAAQDAVKGWNNGLC
LDGTIPATAVSMDVLVSTVATSFNAPTNATTISSNMTARGGPRTLLPRDWCRTEEVLGGD
SCPSLASRCGISGDDLNKYNPQANLCSTLKPKQHVCCTAGTLPDFRPQPQPDGTCNTYKV
NDNDGCWDLANAHYLSQQDIEDFNKNTWGWTGCAGGLQSGQLICLSEGKPPMPAPIPGAT
CGPQVPGTQKPSDETALADLNPCPLNACCDVWGFCGTTVDFCTESPADTGAPGTAKPGTN
GCISNCGMKIVNNGKAPDQVKTVGYFEAFDQLRACLRMSVTEVPANKYSHIHFAFATVTS
DFDVDISNVEDEFRKFVKMRGFQKILSFGGWAFSTDPGTFQRFRDATKPEHRSTFVDNLV
SFMSRQDLDGLDFDWEYPGAPDIPDITPGSADEGRNYLAFLQMLRSKLPIGKSISIALPA
SYWYLKQYPVQKIAKYVDYFIYMTYDLHGQWDVDNKSAIPGCPAGNCLRSHVNITETHDA
MVMITKAGVEARQIVIGVSSYGRSFRMNDASCSGPFCTYAGDRTHSMAYAAPCTATGGYI
ANAELNDIIKDHGNHSIVNSYIDTDSQSNILIYGNPGAVDWVAYMDGGLKANRINWIKGL
NFGGSTDWAIDLQDYSNNENGDDSNDDGVGSDDEAGDDTPTCPPDKNPGTLDGLADQADS
LDPDCVSLFAMDILYSQLLDSLALFQTNSQDYDDKFGWYEKWTKEQIQPRIDDFMKLGDG
KGLQYFDCYWAYTGHDEKKDSCFGMPHIWDMSEGWSIRFDLVDKQGFFDALAAETGIDES
WVTFGDETSDYGCADPGDVRPGAGHVPCRKLYRKKLNYPLKASDDDIHVGNPKKLIEASM
GNVTALRTSLLSSYLSVGLSFYDDGPNDGSATDAVVAYAMPVLQLAEAISSMKDIKDIGE
KAKEEAKKALIFKILSIIFMVIPFVGEALGPLIGSAAAVARIALLIGEAGNGALTVADII
EDPTSAPFAILGLIAGVGGGTGRLSKGEALGEASKARGLLKASDLAKFPQRFRDQDALVQ
KIVKSLCARK

Enzyme Prediction     

EC	3.2.1.14:3	3.2.1.14:3

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	500	860	1.1e-52	0.956081081081081

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
119357	GH18_zymocin_alpha	0.0	501	853	1	345	Zymocin, alpha subunit. Zymocin is a heterotrimeric enzyme that inhibits yeast cell cycle progression. The zymocin alpha subunit has a chitinase activity that is essential for holoenzyme action from the cell exterior while the gamma subunit contains the intracellular toxin responsible for G1 phase cell cycle arrest. The zymocin alpha and beta subunits are thought to act from the cell's exterior by docking to the cell wall-associated chitin, thus mediating gamma-toxin translocation. The alpha subunit has an eight-stranded TIM barrel fold similar to that of family 18 glycosyl hydrolases such as hevamine, chitolectin, and chitobiase.
214753	Glyco_18	7.16e-61	501	852	1	333	Glyco_18 domain.
395573	Glyco_hydro_18	9.68e-51	501	851	1	305	Glycosyl hydrolases family 18.
119365	GH18_chitinase	3.68e-42	523	746	21	267	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.
119349	GH18_chitinase-like	5.20e-39	503	687	2	178	The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE60282.1|CBM18|CBM50|GH18	0.0	1	1269	1	1213
QGA13088.1|CBM18|CBM50|GH18	0.0	1	1269	31	1294
QKX54053.1|CBM18|CBM50|GH18	0.0	32	1270	3	1239
UKZ50509.1|CBM18|CBM50|GH18	0.0	1	1268	1	1248
QMW30533.1|GH18	0.0	488	1261	1	772

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JM7_A	1.78e-26	498	855	2	352	Crystal structure of Ostrinia furnacalis Group IV chitinase [Ostrinia furnacalis],6JMB_A Chain A, ofchtiv-allosamidin [Ostrinia furnacalis]
5WUP_A	3.23e-26	495	856	88	441	Crystal structure of a insect group III chitinase (CAD1) from Ostrinia furnacalis [Ostrinia furnacalis],5WV9_A Crystal structure of a insect group III chitinase complex with (GlcNAc)6 (CAD1-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]
6JM8_A	1.91e-25	498	855	2	352	Crystal structure of Ostrinia furnacalis Group IV chitinase [Ostrinia furnacalis]
3FXY_A	6.44e-25	505	856	6	347	Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FXY_B Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FXY_C Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FXY_D Acidic Mammalian Chinase, Catalytic Domain [Homo sapiens],3FY1_A The Acidic Mammalian Chitinase catalytic domain in complex with methylallosamidin [Homo sapiens],3FY1_B The Acidic Mammalian Chitinase catalytic domain in complex with methylallosamidin [Homo sapiens],3RM4_A AMCase in complex with Compound 1 [Homo sapiens],3RM4_B AMCase in complex with Compound 1 [Homo sapiens],3RM8_A AMCase in complex with Compound 2 [Homo sapiens],3RM8_B AMCase in complex with Compound 2 [Homo sapiens],3RM9_A AMCase in complex with Compound 3 [Homo sapiens],3RM9_B AMCase in complex with Compound 3 [Homo sapiens],3RME_A AMCase in complex with Compound 5 [Homo sapiens],3RME_B AMCase in complex with Compound 5 [Homo sapiens]
2YBT_A	7.01e-25	505	856	10	351	Crystal structure of human acidic chitinase in complex with bisdionin C [Homo sapiens],2YBT_B Crystal structure of human acidic chitinase in complex with bisdionin C [Homo sapiens],2YBT_C Crystal structure of human acidic chitinase in complex with bisdionin C [Homo sapiens],2YBT_D Crystal structure of human acidic chitinase in complex with bisdionin C [Homo sapiens],2YBT_E Crystal structure of human acidic chitinase in complex with bisdionin C [Homo sapiens],2YBT_F Crystal structure of human acidic chitinase in complex with bisdionin C [Homo sapiens],2YBU_A Crystal structure of human acidic chitinase in complex with bisdionin F [Homo sapiens],2YBU_B Crystal structure of human acidic chitinase in complex with bisdionin F [Homo sapiens],2YBU_C Crystal structure of human acidic chitinase in complex with bisdionin F [Homo sapiens],2YBU_D Crystal structure of human acidic chitinase in complex with bisdionin F [Homo sapiens],2YBU_E Crystal structure of human acidic chitinase in complex with bisdionin F [Homo sapiens],2YBU_F Crystal structure of human acidic chitinase in complex with bisdionin F [Homo sapiens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P09805|KTXA_KLULA	2.27e-111	306	967	205	824	Killer toxin subunits alpha/beta OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 PE=1 SV=1
sp|Q95M17|CHIA_BOVIN	3.05e-29	487	856	9	368	Acidic mammalian chitinase OS=Bos taurus OX=9913 GN=CHIA PE=1 SV=1
sp|P36362|CHIT_MANSE	1.38e-25	495	855	18	377	Endochitinase OS=Manduca sexta OX=7130 PE=2 SV=1
sp|Q9BZP6|CHIA_HUMAN	1.62e-23	487	856	9	368	Acidic mammalian chitinase OS=Homo sapiens OX=9606 GN=CHIA PE=1 SV=1
sp|Q6RY07|CHIA_RAT	2.84e-22	487	856	9	368	Acidic mammalian chitinase OS=Rattus norvegicus OX=10116 GN=Chia PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000190	0.999771	CS pos: 17-18. Pr: 0.9798

TMHMM  Annotations     

There is no transmembrane helices in ATEG_10158-t26_1-p1.