CAZyme Information: ATCC64974_76140-t41_1-p1

Basic Information

• Species: Aspergillus niger
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus niger
• CAZyme ID: ATCC64974_76140-t41_1-p1
• CAZy Family: GH76
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
439		48438.19	4.3638

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AnigerN402ATCC64974	11187	N/A	0	11187

• Gene Location: location=OGUI01000013.1:727543-729118(-)

Full Sequence      

MYLLPLTLFLTAAFGVSIPRSPLIPGAQIVPASSTADLRAIGAQHHKYPDRETVTIRASR
NALDDVSSDFLWGLKQANHGGRLLLKQGETYVIGKKLDLTFLDNIEVQLEGEIQFTNNIT
YWQANNFYYDFQKSITFWRWGGQDIKIFGSGVLNGNGQKWYDEFAGKQILDSDNTFYRPI
LFLTDNATRISVEGITQLNSPCWTNFFVRTNDVSFDNVYIHAFSTNASSDPANTDGMDSL
DVDGVSFTNMRIDVGDDCFSPKPNTTNIFVQNMWCNNTHGVSMGSIGQYAGEMDIIENVY
IENVTLLNGQNGARLKAWAGQDVGYGRINNVTYKNIQIQNTDAPIVLDQCYFDINATECA
KYPSAVNITNILFENIWGSSSGKDGKIVADLVCSPDAVCTNITLSNVNLTSPKGTAEIVC
DDIQGGIGVDCVSDESVTR

Enzyme Prediction     

EC	3.2.1.67:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	77	424	8.9e-72	0.9569230769230769

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395231	Glyco_hydro_28	2.72e-44	94	418	14	312	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
215426	PLN02793	1.59e-30	105	425	111	411	Probable polygalacturonase
215120	PLN02188	1.80e-30	180	409	158	374	polygalacturonase/glycoside hydrolase family protein
165802	PLN02155	3.68e-29	217	415	178	369	polygalacturonase
178580	PLN03003	4.20e-29	147	425	117	377	Probable polygalacturonase At3g15720

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAK38430.1|GH28|3.2.1.67	0.0	1	439	1	438
ABD61564.1|GH28|3.2.1.67	0.0	1	439	1	438
BCS12452.1|GH28	0.0	1	439	1	439
BCS00686.1|GH28	0.0	1	439	1	439
QRD92857.1|GH28	1.89e-247	7	431	7	430

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4C2L_A	4.45e-51	61	436	27	387	Crystal structure of endo-xylogalacturonan hydrolase from Aspergillus tubingensis [Aspergillus tubingensis]
6KVE_A	1.56e-22	142	427	75	342	Chain A, Endo-polygalacturonase [Evansstolkia leycettana]
1HG8_A	2.27e-22	73	416	10	338	Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme [Fusarium verticillioides]
6KVH_A	7.27e-22	142	427	75	342	Chain A, endo-polygalacturonase [Evansstolkia leycettana]
7E56_A	8.88e-22	142	427	67	334	Chain A, Endo-polygalacturonase [Evansstolkia leycettana]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q27UB1|PGXB_ASPNG	0.0	1	439	1	438	Exopolygalacturonase B OS=Aspergillus niger OX=5061 GN=pgxB PE=3 SV=1
sp|A2QHG0|PGXB_ASPNC	0.0	1	439	1	438	Probable exopolygalacturonase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=pgxB PE=3 SV=1
sp|B8NX60|PGXB_ASPFN	3.35e-248	7	431	7	430	Probable exopolygalacturonase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=pgxB PE=3 SV=1
sp|Q2TW03|PGXB_ASPOR	4.76e-248	7	431	7	430	Probable exopolygalacturonase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=pgxB PE=3 SV=2
sp|Q0C7H7|PGXB_ASPTN	2.37e-241	23	436	21	435	Probable exopolygalacturonase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=pgxB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.284229	0.715742	CS pos: 15-16. Pr: 0.2749

TMHMM  Annotations     

There is no transmembrane helices in ATCC64974_76140-t41_1-p1.