CAZyme Information: ATCC64974_66700-t41_1-p1

Basic Information

• Species: Aspergillus niger
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus niger
• CAZyme ID: ATCC64974_66700-t41_1-p1
• CAZy Family: GH5
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
741	OGUI01000010.1|CGC1	83253.45	9.1443

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AnigerN402ATCC64974	11187	N/A	0	11187

• Gene Location: location=OGUI01000010.1:1210601-1213177(-)

Full Sequence      

MAESPLDVLLKGNSGRTTRGLLRIIILATIAAAAVSSRLFSVIRFESIIHEFDPWFNFRA
TKYLVSHGFESFWDWFDDRTWHPLGRVTGGTLYPGLMVTSGVIYHVLRFLTIPVDIRNIC
VLLAPGFSGLTALAMYFLTREMATSPSAGLLAAAFMGIVPGYISRSVAGSYDNEAIAIFL
LVFTFFLWIKAVKNGSIMWGSLAALFYGYMVSAWGGYVFITNLIPLHVFVLLCMGRYSSR
IYISYTTWYALGTLASMQIPFVGFLPIRNSDHMSALGVFGLIQLVAFADFVRGFIPGRHF
QRLLTTMIIVVFGIAFVGLVVLTVSGVIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPT
AWPAFFFDLNFLIWLFPAGVYMCFRDLKDEHVFVIIYSVLASYFAGVMVRLMLTLTPIVC
VAAALALSTILDTYVFAKNGPNPRAKANDDTSDGLRSTRKPDVGVTSYLSKAVMTSSVVI
YLLLFVAHCTWVTSNAYSSPSVVLASRLPDGSQHIIDDYREAYYWLRQNTEHNAKIMSWW
DYGYQIGGMADRPTLVDNNTWNNTHIATVGKAMSSREEVSYPILRQHDVDYVLVVFGGLL
GYSGDDINKFLWMVRIAEGIWPDEVKERDFFTARGEYRVDDGATPTMRNSLMYKMSYYNF
NSLFGPGQAVDRVRGSRLPAEGPQLNTLEEAFTSENWIIRIYKVKDLDNLGRDHNNAVAF
DKGHKRKRATKRKGPRVLRTE

Enzyme Prediction     

EC	2.4.99.18:21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	23	663	2e-194	0.8513708513708513

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396873	STT3	1.87e-150	35	498	15	478	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.
224206	Stt3	7.29e-117	35	712	32	737	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].
275016	archaeo_STT3	6.71e-32	51	553	32	547	oligosaccharyl transferase, archaeosortase A system-associated. Members of this protein family occur, one to three members per genome, in the same species of Euryarchaeota as contain the predicted protein-sorting enzyme archaeosortase (TIGR04125) and its cognate protein-sorting signal PGF-CTERM (TIGR04126).
404170	PMT_2	0.002	116	211	24	112	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
396786	PMT	0.005	127	265	92	234	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAS97683.1|GT66	0.0	1	741	1	741
CAK42956.1|GT66	0.0	1	741	1	741
BCS03802.1|GT66	0.0	1	741	1	741
BCS15416.1|GT66	0.0	1	741	1	741
UDD65301.1|GT66	0.0	1	741	1	742

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6C26_A	0.0	21	712	15	690	The Cryo-EM structure of a eukaryotic oligosaccharyl transferase complex [Saccharomyces cerevisiae S288C],6EZN_F Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex [Saccharomyces cerevisiae S288C],7OCI_F Chain F, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C]
6S7T_A	4.85e-289	33	709	83	777	Cryo-EM structure of human oligosaccharyltransferase complex OST-B [Homo sapiens]
6FTG_5	4.98e-266	21	711	17	701	Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes [Canis lupus familiaris],6FTI_5 Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome [Canis lupus familiaris]
6S7O_A	1.00e-265	21	711	17	701	Cryo-EM structure of human oligosaccharyltransferase complex OST-A [Homo sapiens]
6FTJ_5	5.51e-263	21	706	17	696	Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome [Canis lupus familiaris]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O94335|STT3_SCHPO	0.0	22	727	24	738	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=stt3 PE=3 SV=1
sp|P39007|STT3_YEAST	0.0	21	712	15	690	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=STT3 PE=1 SV=2
sp|Q3TDQ1|STT3B_MOUSE	6.88e-290	33	709	80	774	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Mus musculus OX=10090 GN=Stt3b PE=1 SV=2
sp|E2RG47|STT3B_CANLF	6.18e-289	33	709	83	777	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Canis lupus familiaris OX=9615 GN=STT3B PE=1 SV=1
sp|Q8TCJ2|STT3B_HUMAN	2.49e-288	33	709	83	777	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Homo sapiens OX=9606 GN=STT3B PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000015	0.000001

TMHMM  Annotations     

Start	End
20	42
90	112
119	138
148	168
175	192
212	234
241	263
273	295
308	330
362	384
391	413
418	437
465	487