CAZyme Information: ATCC64974_4680-t41_1-p1

Basic Information

• Species: Aspergillus niger
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus niger
• CAZyme ID: ATCC64974_4680-t41_1-p1
• CAZy Family: GH26
• CAZyme Description: unspecified product

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
841		93950.25	5.0967

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AnigerN402ATCC64974	11187	N/A	0	11187

• Gene Location: location=OGUI01000001.1:1426371-1428896(+)

Full Sequence      

MRGLNLFQLILALLLSMVAAEDGYDGWLRYAPVSCDLRCRQALPSHVVLLNSTKGSPIET
AGRELKAGFQSILSTNLTSRPFQCNSSTSILVATLDEYRQRCRDINVPELDPDGFWLQSE
GDTVRILGKDARGALYGAYEYLAMVAQRNFSRVAYATSPHAPIRWVNQWDNMDGSIERGY
GGASIFFKDGTVVEDMAPVEQYARLLASIRINAIVVNNVNANATLLLPENMKGLGRIADA
CRPYGVQIGISLNFASPEDLGGLNTYDPLDPGVIAWWQNITDSLYTYVPDMAGYLVKADS
EGQPGPDTYNRTLSQGANLFARALQPYGGVLMYRAFVYDDNLNESDWKADRAKAAVEYFK
DLDGQFEENVVIQIKYGPIDFQVREPTSPLFANLYHTNTAIELEVSQEYLGQQCHLVYLP
PLWKTVLDFDLRVDHKPSMVRDIISGQRFNRTLGGWAAVVNVGTNRTWLGSHLAMSNLYA
YGRLAWSPTDESEQILEDWTRLTFGQNHHVINTISDMSMTSWPAYENYTGNLGIQTLTDI
LYTHYGPNPATQDNNGWGQWTRADHDSVGMDRTIRNGTGYTGQYPEEVARVYESLESTPD
DLVLWFHHVPWTHRLHSGVTVIQHFYNAHYAGAEAAHGFVRQWESLEGLIDRERYEAMRS
RLVYQAGHSIVWRDAINNFYYNMTGIPDVAGRVGHHPWRIEAESMRLDGYQTYTVSPFEA
ASNTTAIITTSNSTTGTARTSIKAPSGVYDIGVNYYDLYGGQSKWTLSVGDKVVGQWLGD
MEHNSLGHTPSIYLDGHSATRITFHGVGIRQGDQLKIVGEANGVEPAPLDYIVLLPPGLV
D

Enzyme Prediction     

EC	3.2.1.131:5	3.2.1.139:5

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH67	22	692	1.9e-274	0.9985052316890882

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
226187	AguA2	0.0	17	692	1	679	Alpha-glucuronidase [Carbohydrate transport and metabolism].
400047	Glyco_hydro_67M	0.0	144	467	1	324	Glycosyl hydrolase family 67 middle domain. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the central catalytic domain of alpha-glucuronidase.
400039	Glyco_hydro_67C	2.22e-138	470	693	2	223	Glycosyl hydrolase family 67 C-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the C terminal region of alpha-glucuronidase which is mainly alpha-helical. It wraps around the catalytic domain (pfam07488), making additional interactions both with the N-terminal domain (pfam03648) of its parent monomer and also forming the majority of the dimer-surface with the equivalent C-terminal domain of the other monomer of the dimer.
397627	Glyco_hydro_67N	7.34e-38	26	141	1	120	Glycosyl hydrolase family 67 N-terminus. Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. This family represents the N-terminal region of alpha-glucuronidase. The N-terminal domain forms a two-layer sandwich, each layer being formed by a beta sheet of five strands. A further two helices form part of the interface with the central, catalytic, module (pfam07488).
271143	CBM6-CBM35-CBM36_like	4.00e-08	699	834	1	124	Carbohydrate Binding Module 6 (CBM6) and CBM35_like superfamily. Carbohydrate binding module family 6 (CBM6, family 6 CBM), also known as cellulose binding domain family VI (CBD VI), and related CBMs (CBM35 and CBM36). These are non-catalytic carbohydrate binding domains found in a range of enzymes that display activities against a diverse range of carbohydrate targets, including mannan, xylan, beta-glucans, cellulose, agarose, and arabinans. These domains facilitate the strong binding of the appended catalytic modules to their dedicated, insoluble substrates. Many of these CBMs are associated with glycoside hydrolase (GH) domains. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold. CBM36s are calcium-dependent xylan binding domains. CBM35s display conserved specificity through extensive sequence similarity, but divergent function through their appended catalytic modules. This alignment model also contains the C-terminal domains of bacterial insecticidal toxins, where they may be involved in determining insect specificity through carbohydrate binding functionality.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAC38119.1|GH67	0.0	1	841	1	841
ALI53917.1|GH67	0.0	1	841	1	841
CAK42141.1|GH67	0.0	1	841	1	841
BCR99523.1|GH67	0.0	1	841	1	841
BCS11820.1|GH67	0.0	1	841	1	841

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1K9D_A	9.68e-195	23	692	4	675	The 1.7 A crystal structure of alpha-D-glucuronidase, a family-67 glycoside hydrolase from Bacillus stearothermophilus T-1 [Geobacillus stearothermophilus],1L8N_A The 1.5A crystal structure of alpha-D-glucuronidase from Bacillus stearothermophilus T-1, complexed with 4-O-methyl-glucuronic acid and xylotriose [Geobacillus stearothermophilus],1MQQ_A THE CRYSTAL STRUCTURE OF ALPHA-D-GLUCURONIDASE FROM BACILLUS STEAROTHERMOPHILUS T-1 COMPLEXED WITH GLUCURONIC ACID [Geobacillus stearothermophilus]
1MQP_A	1.93e-194	23	692	4	675	The Crystal Structure Of Alpha-D-Glucuronidase From Bacillus Stearothermophilus T-6 [Geobacillus stearothermophilus]
1MQR_A	5.46e-194	23	692	4	675	Chain A, ALPHA-D-GLUCURONIDASE [Geobacillus stearothermophilus]
1K9E_A	1.09e-193	23	692	4	675	Chain A, alpha-D-glucuronidase [Geobacillus stearothermophilus],1K9F_A Chain A, alpha-D-glucuronidase [Geobacillus stearothermophilus]
1GQI_A	6.62e-142	21	723	1	703	Structure of Pseudomonas cellulosa alpha-D-glucuronidase [Cellvibrio japonicus],1GQI_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase [Cellvibrio japonicus],1GQJ_A Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with xylobiose [Cellvibrio japonicus],1GQJ_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with xylobiose [Cellvibrio japonicus],1GQK_A Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid [Cellvibrio japonicus],1GQK_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid [Cellvibrio japonicus],1GQL_A Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid and xylotriose [Cellvibrio japonicus],1GQL_B Structure of Pseudomonas cellulosa alpha-D-glucuronidase complexed with glucuronic acid and xylotriose [Cellvibrio japonicus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5AQZ4|AGUA_EMENI	0.0	7	841	4	847	Alpha-glucuronidase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=aguA PE=1 SV=1
sp|Q0CJP9|AGUA_ASPTN	0.0	9	841	8	841	Probable alpha-glucuronidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=aguA PE=3 SV=1
sp|Q2UFP4|AGUA_ASPOR	0.0	7	841	5	835	Probable alpha-glucuronidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=aguA PE=3 SV=1
sp|Q4WW45|AGUA_ASPFU	0.0	3	841	4	840	Probable alpha-glucuronidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=aguA PE=3 SV=1
sp|A1DD80|AGUA_NEOFI	0.0	3	841	4	840	Probable alpha-glucuronidase A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=aguA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000175	0.999787	CS pos: 20-21. Pr: 0.9775

TMHMM  Annotations     

There is no transmembrane helices in ATCC64974_4680-t41_1-p1.