CAZyme Information: ASPZODRAFT_24885-t33_1-p1

Basic Information

• Species: Penicilliopsis zonata
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Penicilliopsis; Penicilliopsis zonata
• CAZyme ID: ASPZODRAFT_24885-t33_1-p1
• CAZy Family: GH5
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
585		61922.06	3.8715

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PzonataCBS506.65	10027	1073090	158	9869

• Gene Location: location=KV878340:1514159-1516457(+)

Full Sequence      

MLKINSIWMLPAFWAFVRAQQVGTYTSEVHPTLTWTSYSSASAYATQTGSVVIDANWRWT
HDVDSSTNCYTGNEWDTTLCPDDVTCAANCALDGADYEGTYGVTTSGSEVKLDFVTGSNV
GSRLYLMENDDTYQVFKLLNREFTFDVDVSELPCGLNGALYFVTMDSDGGMARFPNNTAG
AKYGTGYCDAQCPRDLKFIDGQGNSEGWVPSSNNDNTGVGDHGSCCVEMDVWEANSISTA
LTPHSCDTATQTLCEGDDCGGTYSSDRYGGTCDPDGCDFNPYRLGNLSYYGPGETVDTNS
VFTVVTQFITDDGTDTGTLSEIKRYYVQNDVVISQATTSATGVSGYDSITADFCTAEKTA
FGDTNVWSTHGGFTSMTEAMTNGMVLVFSLWDDYYADMLWLDSNYPTNESASDPGVARGT
CSTSSGVPATVEANNGTAYVTYSNVKFGPIGSTFSGGTGTTTDVDTGSGSSGSSSSSIST
LVTTTRAASTSSTSTSTSTSTSTSTSTSITTSVSSQTTTTAVAVTTTSSGSTAVASQYGQ
CGGDNWTGATECASGYTCTYQNPYYSQCLARKIVETRVKTNIVFL

Enzyme Prediction     

EC	3.2.1.176:83	3.2.1.132:6	3.2.1.4:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH7	21	454	2.4e-184	0.9951807228915662

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
153432	GH7_CBH_EG	0.0	27	448	1	386	Glycosyl hydrolase family 7. Glycosyl hydrolase family 7 contains eukaryotic endoglucanases (EGs) and cellobiohydrolases (CBHs) that hydrolyze glycosidic bonds using a double-displacement mechanism. This leads to a net retention of the conformation at the anomeric carbon. Both enzymes work synergistically in the degradation of cellulose,which is the main component of plant cell wall, and is composed of beta-1,4 linked glycosyl units. EG cleaves the beta-1,4 linkages of cellulose and CBH cleaves off cellobiose disaccharide units from the reducing end of the chain. In general, the O-glycosyl hydrolases are a widespread group of enzymes that hydrolyze the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycoside hydrolase family 7.
395677	Glyco_hydro_7	0.0	22	450	1	431	Glycosyl hydrolase family 7.
395595	CBM_1	7.67e-13	537	565	1	29	Fungal cellulose binding domain.
197593	fCBD	1.83e-12	537	569	2	34	Fungal-type cellulose-binding domain. Small four-cysteine cellulose-binding domain of fungi

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QEQ56122.1|CBM1|GH7	7.49e-246	9	569	11	536
ADO21450.1|CBM1|GH7	5.74e-245	20	569	1	515
AEF58998.1|CBM1|GH7	3.51e-244	9	569	11	536
BCR93402.1|CBM1|GH7	1.00e-243	9	569	11	536
BCS06046.1|CBM1|GH7	1.00e-243	9	569	11	536

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1Q9H_A	3.01e-238	21	455	2	435	Chain A, cellobiohydrolase I catalytic domain [Rasamsonia emersonii],3PFJ_A Chain A, Cellobiohydrolase 1 catalytic domain [Rasamsonia emersonii],3PFX_A Chain A, Cellobiohydrolase 1 catalytic domain [Rasamsonia emersonii],3PFZ_A Chain A, Cellobiohydrolase 1 catalytic domain [Rasamsonia emersonii],3PL3_A Chain A, Cellobiohydrolase 1 catalytic domain [Rasamsonia emersonii]
4V1Z_A	9.60e-226	21	457	2	440	The 3-D structure of the cellobiohydrolase, Cel7A, from Aspergillus fumigatus [Aspergillus fumigatus],4V20_A The 3-D structure of the cellobiohydrolase, Cel7A, from Aspergillus fumigatus, disaccharide complex [Aspergillus fumigatus]
4ZZT_A	8.10e-217	21	456	2	437	Geotrichum candidum Cel7A structure complex with thio-linked cellotriose at 1.56A [Geotrichum candidum],4ZZU_A Geotrichum candidum Cel7A structure complex with thio-linked cellotetraose at 1.4A [Geotrichum candidum],4ZZV_A Geotrichum candidum Cel7A apo structure at 1.4A [Geotrichum candidum],4ZZW_A Geotrichum candidum Cel7A structure complex with cellobiose at 1.5A [Geotrichum candidum],5AMP_A Geotrichum candidum Cel7A apo structure at 2.1A [Geotrichum candidum]
4XEB_A	2.02e-214	21	457	2	436	The structure of P. funicolosum Cel7A [Talaromyces funiculosus]
4ZZP_A	9.27e-209	21	454	2	436	Dictyostelium purpureum cellobiohydrolase Cel7A apo structure [Dictyostelium purpureum],4ZZP_B Dictyostelium purpureum cellobiohydrolase Cel7A apo structure [Dictyostelium purpureum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9UVS8|CBHB_ASPNG	1.67e-242	9	569	11	536	1,4-beta-D-glucan cellobiohydrolase B OS=Aspergillus niger OX=5061 GN=cbhB PE=2 SV=1
sp|A2QAI7|CBHB_ASPNC	1.67e-242	9	569	11	536	Probable 1,4-beta-D-glucan cellobiohydrolase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=cbhB PE=3 SV=1
sp|Q4WM08|CBHB_ASPFU	2.64e-233	18	569	25	532	Probable 1,4-beta-D-glucan cellobiohydrolase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbhB PE=1 SV=1
sp|B0Y8K2|CBHB_ASPFC	2.64e-233	18	569	25	532	Probable 1,4-beta-D-glucan cellobiohydrolase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=cbhB PE=3 SV=1
sp|Q0CMT2|CBHB_ASPTN	3.42e-231	5	569	9	541	Probable 1,4-beta-D-glucan cellobiohydrolase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=cbhB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000339	0.999641	CS pos: 19-20. Pr: 0.9615

TMHMM  Annotations     

There is no transmembrane helices in ASPZODRAFT_24885-t33_1-p1.