CAZyme Information: ASPZODRAFT_20739-t33_1-p1

Basic Information

• Species: Penicilliopsis zonata
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Penicilliopsis; Penicilliopsis zonata
• CAZyme ID: ASPZODRAFT_20739-t33_1-p1
• CAZy Family: GH43
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1071	KV878367|CGC1	117234.13	4.7353

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PzonataCBS506.65	10027	1073090	158	9869

• Gene Location: location=KV878367:11417-15472(+)

Full Sequence      

MPRAGYCGFGPSFCGSGCVSNCDATSECGPFANPPGKTCPLNTCCSQYGFCGTTEEFCGA
GCQSNCVLHPQPPPGSPNNQTLSKVIGYYEAWSARSKCHQTSPKDLPVNGLTHLNYAFAY
IDPSTWTLTTMDGATPLSLFDDLAALKATNPNLNIFISVGGWSFSDNDTATQPIFGNLAR
EETNRRTFAKNALAFLNQYGFDGIDIDWEYPGAPDRGGSKDDTQNYVHLLEALRTTFTNS
GRKLGVTFTAPSSYWYLRWFDLPGMLKYADWMNWMTYDLHGVWDSSNPMHVPCNPSGSIV
QGHTNLTEIKTAAELLWRVRVNPAQVSLGFAFYGRAFSLQDPGCTSPGCPFSGGADPGVC
TGTSGYLSYYEIEQILSSNPDINIVHDNEAAVKYFAWSAGHIKANTKNRKMAPSQWISYD
DKDTFGQKKEWTDEIGIGGSLIWASDLDDYDWKAQSGLTGIKVMEMGPGPVQTKEVLDGS
LAKKCYLGAESVYRMPDGSSNPGILATTCRPDTAVGYDSHGCANGDDSSCVQPICCPPQA
GLKNCQWRGDSSDGICNGECNVGEVKVASSSWGELDMQKCKRGEKVLCCEAGYMVSLIDG
CRWTSNGDQCSSTEDSVAIALDLSGRLANPEHAQNYYCCSNEIQPTPLYNCHWVGEGDCD
DVNCAANEVTLWRDALGDKPSACPWSKEKALCCQPHAEALAYLLCDIDLCKDIPEWCGDE
DGIWDDEEGNEDYLHIDVLKGRPGEPREMIIQIGKLLGSSMEQALKMTSRGYAPGFKTFQ
GDGWRTMARLGGYKMGKDVCSATIVSFVKKEDILETGWNAEHLQEIQMVGQLIKTAVTGT
LPSGKTMQSSILDAVKLINGWNKVYDTTLPRIGALVTDVAGWTPPVTPNDRIFEVIGSFA
YRTGISFVERDINSAKLNLVKGYNVMALRRFKGFLDAVNLGNETAAKVIATTLQKTIGVF
EYLNAEASLGGLTEARTAFLMEIARADKYMPELKGLLSIWKEFEPDFYATMVRFAASWIN
ARIQQVHDRFPAGGSIDNPAVLKLLYDVKLISIATNRIKFDSQLDYDNVNV

Enzyme Prediction     

No EC number prediction in ASPZODRAFT_20739-t33_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	83	454	6.8e-62	0.956081081081081

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
214753	Glyco_18	6.08e-86	84	448	1	334	Glyco_18 domain.
119357	GH18_zymocin_alpha	1.79e-83	84	448	1	345	Zymocin, alpha subunit. Zymocin is a heterotrimeric enzyme that inhibits yeast cell cycle progression. The zymocin alpha subunit has a chitinase activity that is essential for holoenzyme action from the cell exterior while the gamma subunit contains the intracellular toxin responsible for G1 phase cell cycle arrest. The zymocin alpha and beta subunits are thought to act from the cell's exterior by docking to the cell wall-associated chitin, thus mediating gamma-toxin translocation. The alpha subunit has an eight-stranded TIM barrel fold similar to that of family 18 glycosyl hydrolases such as hevamine, chitolectin, and chitobiase.
119351	GH18_chitolectin_chitotriosidase	3.75e-73	85	450	1	343	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.
395573	Glyco_hydro_18	3.57e-67	84	448	1	307	Glycosyl hydrolases family 18.
119365	GH18_chitinase	3.40e-65	85	448	1	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALI93561.1|CBM18|GH18	0.0	3	1044	75	1130
ANH56415.1|CBM18|GH18	0.0	3	1044	75	1130
UKZ93576.1|GH18	0.0	70	1059	20	1012
QDJ94336.1|CBM18|GH18	2.27e-269	4	1025	7	1018
AIT18874.1|CBM18|GH18	1.17e-257	3	1027	58	1042

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JM7_A	6.40e-48	85	450	6	352	Crystal structure of Ostrinia furnacalis Group IV chitinase [Ostrinia furnacalis],6JMB_A Chain A, ofchtiv-allosamidin [Ostrinia furnacalis]
5WUP_A	2.46e-47	84	450	94	440	Crystal structure of a insect group III chitinase (CAD1) from Ostrinia furnacalis [Ostrinia furnacalis],5WV9_A Crystal structure of a insect group III chitinase complex with (GlcNAc)6 (CAD1-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]
5WUS_A	3.88e-47	84	536	3	417	Crystal structure of a insect group III chitinase (CAD2) from Ostrinia furnacalis [Ostrinia furnacalis],5WVH_A Crystal structure of an insect group III chitinase complex with (GlcNAc)6 (CAD2-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]
6JM8_A	7.34e-47	85	450	6	352	Crystal structure of Ostrinia furnacalis Group IV chitinase [Ostrinia furnacalis]
5WV8_A	2.74e-46	84	450	94	440	Crystal structure of a mutant insect group III chitinase (CAD1-E217L) from Ostrinia furnacalis [Ostrinia furnacalis],5WVB_A Crystal structure of a mutant insect group III chitinase complex with (GlcNAc)6 (CAD1-E217L-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q13231|CHIT1_HUMAN	3.48e-42	81	450	20	365	Chitotriosidase-1 OS=Homo sapiens OX=9606 GN=CHIT1 PE=1 SV=1
sp|Q91XA9|CHIA_MOUSE	1.78e-41	85	450	24	367	Acidic mammalian chitinase OS=Mus musculus OX=10090 GN=Chia PE=1 SV=2
sp|Q6RY07|CHIA_RAT	3.24e-41	85	450	24	367	Acidic mammalian chitinase OS=Rattus norvegicus OX=10116 GN=Chia PE=2 SV=1
sp|Q9D7Q1|CHIT1_MOUSE	6.74e-41	81	450	20	363	Chitotriosidase-1 OS=Mus musculus OX=10090 GN=Chit1 PE=1 SV=2
sp|Q95M17|CHIA_BOVIN	1.92e-40	84	450	23	367	Acidic mammalian chitinase OS=Bos taurus OX=9913 GN=CHIA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999052	0.000986

TMHMM  Annotations     

There is no transmembrane helices in ASPZODRAFT_20739-t33_1-p1.