CAZyme Information: ASPWEDRAFT_116696-t33_1-p1

Basic Information

• Species: Aspergillus wentii
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus wentii
• CAZyme ID: ASPWEDRAFT_116696-t33_1-p1
• CAZy Family: AA7
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
654		72713.51	4.2318

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AwentiiDTO134E9	12659	1073089	224	12435

• Gene Location: location=KV878215:1666049-1668283(+)

Full Sequence      

MAEHEDANLPHIQLNSGQHDPYVDAQETLPPTRVSSIGRALTPGLARSPSEATYHTVYGS
PNPVAESSEFLLPPRPYLIQEELEQQRRSPRASLLSSRRDSWGSLASLEGSRAYPFSPYV
DSRPVSRAFSEDSDINTQTVAEKYDIVPVEGRFPGDPEDDDDIHNPDPNDRERNCDIWNR
RGVLNVGGLILISVGFLVLFVGYPVITAVIGVTKKHDLCDPGDTLCLDVGKRELLSNIRT
GLIDPDTPKSALTKTAEDGKEWKLVFSDEFTKPGRTFYDEDDPYYQAVDIWYGVTQDLEW
YDPDAVTTKDGVLELRFDAFKNHDMDYRSGMLQSWNKMCFTGGRLEASISLPGSGDVSGF
WPGFWAMGNLGRPGYAATTDGMWPYSYYDGCDAGITPNQSSTDGINWLPGMRLPACTCDS
AEHPTPGKSRSAPEIDVIEASVRPLNGNPNAVIGSVSQSLQMAPFDIWYMPDYEFAAVYD
PTITEINDYRGGTYQQAMSGLTNLNNDWYDGNEYQVYAFEYSPGGDGSVTWFVGDDKTWT
LDGRAIGPNGNVGQRVIPMEPLSIVMNLGMATSFAPINESIANLMPGFMRFDYIRIYQDP
DNISVTCDPPGYETTDYIAKHGEAYNNVNKTTWYVQHSAGYDWPQNSFMHQCQG

Enzyme Prediction     

No EC number prediction in ASPWEDRAFT_116696-t33_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	268	597	3.9e-146	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	153	652	4	500	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	2.10e-162	264	598	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	1.81e-68	232	611	19	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	3.99e-17	266	597	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185691	GH16_Strep_laminarinase_like	3.61e-05	262	384	2	126	Streptomyces laminarinase-like, member of glycosyl hydrolase family 16. Proteins similar to Streptomyces sioyaensis beta-1,3-glucanase (laminarinase) present in Actinomycetales as well as Peziomycotina. Laminarinases belong to glycosyl hydrolase family 16 and hydrolyze the glycosidic bond of the 1,3-beta-linked glucan, a major component of fungal and plant cell walls and the structural and storage polysaccharides (laminarin) of marine macro-algae. Members of the GH16 family have a conserved jelly roll fold with an active site channel.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCR96345.1|GH16_2	0.0	10	652	11	648
BCS08859.1|GH16_2	0.0	10	652	11	642
CAL00612.1|GH16_2	0.0	10	652	11	653
BAE59904.1|GH16_2	0.0	8	652	12	657
QRD86509.1|GH16_2	0.0	8	652	12	657

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2HYK_A	1.24e-06	264	368	9	110	The crystal structure of an endo-beta-1,3-glucanase from alkaliphilic Nocardiopsis sp.strain F96 [Nocardiopsis sp. F96]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O13941|YEPB_SCHPO	1.51e-166	158	652	132	627	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1
sp|P32486|KRE6_YEAST	5.19e-165	158	652	217	711	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|P87024|SKN1_CANAX	1.92e-157	158	649	246	735	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P33336|SKN1_YEAST	3.69e-157	158	652	257	763	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1
sp|P87023|KRE6_CANAX	7.34e-155	122	649	200	738	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000058	0.000000

TMHMM  Annotations     

Start	End
183	205