CAZyme Information: ASPNIDRAFT2_1164138-t41_1-p1

Basic Information

• Species: Aspergillus niger
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus niger
• CAZyme ID: ASPNIDRAFT2_1164138-t41_1-p1
• CAZy Family: GH47
• CAZyme Description: Carbohydrate-Binding Module Family 18/ Glycoside Hydrolase Family 16 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
403	ACJE01000009.1|CGC15	43614.99	4.0659

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AnigerATCC1015	11910	380704	6	11904

• Gene Location: transcript_product=Carbohydrate-Binding Module Family 18/ Glycoside Hydrolase Family 16 protein

Full Sequence      

MRHIPAWFVACLAAATASASAPTCSTDSKCPEEYPCCYSGQCGVGTYCLGGCNPLESYSL
DSCTPEPICKNQTYTFTNMDNAVLYDHYLGNASEYDWVYSGYPKIKNESLWLTMPNGTTG
SLYSLNHYIWYGKISAKIKSSRTQGVVTGFILMSDDFDEIDLEWVGYNLSSAQTNFYFQG
VENYTNEVNAAVSTGNTFDEWHTYTIDWKPDVLEWLIDGTVMRTLTKNSTYNATSGIYQY
PQTPSRLEMSIWPGGSEAEGQGTIEWAGGLIDWDSTDIKEYGYFYAQYKDITVECYPAPS
GTIDKGDTSYVYTSDSGLSDTIELSGNSTVLYNLQDTGLNMTAGTKMSDSATASGSSSGA
SSTGGSSSSSSSKKESAASGYGERVMEGSVLAAVGAVVALGLF

Enzyme Prediction     

No EC number prediction in ASPNIDRAFT2_1164138-t41_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	99	272	1.5e-73	0.9885057471264368

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185692	GH16_fungal_CRH1_transglycosylase	3.93e-96	88	296	3	202	glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
395585	Glyco_hydro_16	1.22e-49	104	268	7	168	Glycosyl hydrolases family 16.
211314	ChtBD1_GH16	2.15e-19	23	67	2	47	Hevein or Type 1 chitin binding domain subfamily that co-occurs with family 16 glycosyl hydrolases. This subfamily includes Saccharomyces cerevisiae Utr2p, also known as Crh2p, which participates in the cross-linking of chitin to beta(1-3)- and beta(1-6) glucan in the cell wall, and S. cerevisiae Crr1p, a putative transglycosidase which is needed for proper spore wall assembly. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements.
185683	Glyco_hydrolase_16	2.58e-17	113	273	49	206	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
225182	BglS	4.04e-17	129	281	116	265	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAK96563.1|CBM18|GH16_19	2.56e-287	1	403	1	401
BCS07549.1|CBM18|GH16_19	5.97e-282	1	402	1	402
BCR94975.1|CBM18|GH16_19	5.97e-282	1	402	1	402
CAK39569.1|CBM18|GH16_19	6.40e-148	1	356	2	358
BCR94459.1|CBM18|GH16_19	9.67e-141	1	356	2	360

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6IBU_A	4.47e-34	52	317	4	237	Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBU_B Apo Crh5 transglycosylase [Aspergillus fumigatus Af293],6IBW_A Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293],6IBW_B Crh5 transglycosylase in complex with NAG [Aspergillus fumigatus Af293]
3D6E_A	6.50e-18	131	268	59	181	Crystal structure of the engineered 1,3-1,4-beta-glucanase protein from Bacillus licheniformis [Bacillus licheniformis],3D6E_B Crystal structure of the engineered 1,3-1,4-beta-glucanase protein from Bacillus licheniformis [Bacillus licheniformis]
1GBG_A	1.16e-17	131	268	72	194	Bacillus Licheniformis Beta-Glucanase [Bacillus licheniformis]
3O5S_A	7.48e-16	131	268	96	218	Crystal Structure of the endo-beta-1,3-1,4 glucanase from Bacillus subtilis (strain 168) [Bacillus subtilis]
1CPM_A	5.82e-15	131	307	14	178	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WI46|CRF2_ASPFU	7.56e-144	9	356	10	357	Probable glycosidase crf2 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=crf2 PE=1 SV=1
sp|Q5AJC0|UTR2_CANAL	3.52e-100	17	340	22	354	Extracellular glycosidase UTR2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=UTR2 PE=1 SV=1
sp|P32623|CRH2_YEAST	8.11e-98	20	350	23	356	Probable glycosidase CRH2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=UTR2 PE=1 SV=3
sp|Q05790|CRR1_YEAST	8.32e-65	23	296	30	354	Probable glycosidase CRR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CRR1 PE=2 SV=1
sp|Q75A41|CRR1_ASHGO	5.38e-62	24	296	31	362	Probable glycosidase CRR1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CRR1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000255	0.999727	CS pos: 19-20. Pr: 0.9742

TMHMM  Annotations     

There is no transmembrane helices in ASPNIDRAFT2_1164138-t41_1-p1.