CAZyme Information: ASPNIDRAFT2_1134428-t41_1-p1

Basic Information

• Species: Aspergillus niger
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus niger
• CAZyme ID: ASPNIDRAFT2_1134428-t41_1-p1
• CAZy Family: GH135
• CAZyme Description: Glycoside Hydrolase Family 28 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
427		46388.56	4.0705

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AnigerATCC1015	11910	380704	6	11904

• Gene Location: location=ACJE01000009.1:203043-204860(-)

Full Sequence      

MRSILFILFSLFAGLAAGQLIGPVGPTTQLEDKDIECNILDYGGVADNETDVATSIETTF
TECVLNNPKSRLVIPEGDYLIKRSVVLSNGTNWAFQLDGLITAAYGGNWTVDRELILQGF
AGEQIINSTINGEGDGKFLLDVLVIVNAVDFEFYSSNGLGAFQGQGYIYRNLANTDRPRL
VTNRDECVSVKSPSHHALVENLVCNQAGSGVSIGSLNVSAEISNIEARNISIIQGNNIAF
IKTYPGGSGYVKDVTFENFRSLNSLYGLDINQYWQNTWEPDTGSVTLSNLVFKNFSGSVA
DGALRPPLYLFASDLTFATNVTVEEFSVWTETGTTVVNKISNIFGTGDDSYEENDGIESL
QSGESPYTYTSTYTITASPTNWQAPSTPTWALPSTGYGTASPIPVYTPAPLWRPGGIDYN
LHYWGSF

Enzyme Prediction     

No EC number prediction in ASPNIDRAFT2_1134428-t41_1-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	181	336	3.4e-29	0.4646153846153846

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395231	Glyco_hydro_28	7.11e-14	181	328	156	303	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
177865	PLN02218	1.92e-11	185	300	264	383	polygalacturonase ADPG
215540	PLN03010	1.85e-10	185	350	229	396	polygalacturonase
215426	PLN02793	7.35e-10	180	300	244	369	Probable polygalacturonase
178580	PLN03003	2.20e-07	180	298	205	330	Probable polygalacturonase At3g15720

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ABD67155.1|GH28	2.16e-271	1	427	1	455
ABD67157.1|GH28	7.20e-270	1	427	1	455
CAK96548.1|GH28	2.19e-264	1	427	1	474
QQK39531.1|GH28	2.71e-243	15	427	14	489
BAE57693.1|GH28	3.21e-232	7	427	4	487

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RMG_A	1.25e-53	19	420	1	418	Rhamnogalacturonase A From Aspergillus Aculeatus [Aspergillus aculeatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q5ARE6|RHGA_EMENI	3.19e-58	15	420	14	436	Rhamnogalacturonase A OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rhgA PE=2 SV=2
sp|A2QYE5|RHGA_ASPNC	4.42e-58	15	415	15	432	Probable rhamnogalacturonase A OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rhgA PE=3 SV=1
sp|P87160|RHGA_ASPNG	2.38e-57	15	415	15	432	Rhamnogalacturonase A OS=Aspergillus niger OX=5061 GN=rhgA PE=1 SV=1
sp|B8NKB9|RHGE_ASPFN	5.27e-56	18	420	21	443	Probable rhamnogalacturonase E OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rhgE PE=3 SV=2
sp|Q00001|RHGA_ASPAC	6.31e-55	1	420	1	436	Rhamnogalacturonase A OS=Aspergillus aculeatus OX=5053 GN=rhgA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000233	0.999724	CS pos: 18-19. Pr: 0.9718

TMHMM  Annotations     

There is no transmembrane helices in ASPNIDRAFT2_1134428-t41_1-p1.