CAZyme Information: ASPNIDRAFT2_1129981-t41_1-p1

Basic Information

• Species: Aspergillus niger
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus niger
• CAZyme ID: ASPNIDRAFT2_1129981-t41_1-p1
• CAZy Family: GH12
• CAZyme Description: Glycosyltransferase Family 33 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
459	ACJE01000021.1|CGC15	50941.87	6.6130

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AnigerATCC1015	11910	380704	6	11904

• Gene Location: location=ACJE01000021.1:2816495-2818009(-)

Full Sequence      

MIESLISVAFCLSTALTIGILLLPSRHESSSVTSQNAANEPKTTVQILVLGDIGRSPRMQ
YHALSVARNGGQVDMIGYHESDVHPEISSHPRISIVALPPHPSCLQTSNKLLFLLFGPLK
VLFQIVCLFWILAYRTKPAKWLLVQNPPSIPTLAIASLVCFLRHTKLLIDWHNFGYTILA
LKLGNSHPLVRFSKLYEKIFCQYATAHLCVTNAMASVLKEEFALKAPILPLHDRPADHFQ
PILDDRVRQDFLRSLSETETVRPIIGSDKLRVLVSSTSWTADEDFSLLIEALCRYSDLAE
ASNPDLPSILAIITGKGPQREMYLKQIADLEKNGKLQKATIRTAWLSMEDYARLLASASL
GVSLHTSSSGVDLPMKVVDMFGAGLPVVGWNCFEAWPELVTEGRNGRGFGSSDELVEHLV
ELFGNQEALEELRTGAREESSRRWDDEWNPVAGKLLGLV

Enzyme Prediction     

EC	2.4.1.142:10	2.4.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT33	42	454	1.8e-164	0.9952941176470588

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
340843	GT33_ALG1-like	0.0	41	456	2	411	chitobiosyldiphosphodolichol beta-mannosyltransferase and similar proteins. This family is most closely related to the GT33 family of glycosyltransferases. The yeast gene ALG1 has been shown to function as a mannosyltransferase that catalyzes the formation of dolichol pyrophosphate (Dol-PP)-GlcNAc2Man from GDP-Man and Dol-PP-Glc-NAc2, and participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. In humans ALG1 has been associated with the congenital disorders of glycosylation (CDG) designated as subtype CDG-Ik.
215155	PLN02275	1.43e-147	45	423	7	371	transferase, transferring glycosyl groups
223515	RfaB	4.02e-17	50	456	14	371	Glycosyltransferase involved in cell wall bisynthesis [Cell wall/membrane/envelope biogenesis].
340831	GT4_PimA-like	1.74e-14	149	458	90	364	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
340825	GT4_WbuB-like	5.48e-13	51	456	12	391	Escherichia coli WbuB and similar proteins. This family is most closely related to the GT1 family of glycosyltransferases. WbuB in E. coli is involved in the biosynthesis of the O26 O-antigen. It has been proposed to function as an N-acetyl-L-fucosamine (L-FucNAc) transferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAK39183.1|GT33	0.0	1	459	1	459
BCS01601.1|GT33	0.0	1	459	1	459
BCS13317.1|GT33	0.0	1	459	1	459
BAE65834.1|GT33	2.57e-263	1	459	1	459
QRD93281.1|GT33	1.48e-262	1	459	1	459

PDB Hits     

ASPNIDRAFT2_1129981-t41_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|D4AZD1|ALG1_ARTBC	1.54e-151	9	459	11	446	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01551 PE=3 SV=1
sp|O13933|ALG1_SCHPO	2.76e-103	42	456	24	420	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=alg1 PE=3 SV=2
sp|Q6C3K2|ALG1_YARLI	1.81e-102	47	458	44	440	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=ALG1 PE=3 SV=1
sp|Q8L7M0|TUN_ARATH	9.22e-94	47	459	9	458	UDP-glycosyltransferase TURAN OS=Arabidopsis thaliana OX=3702 GN=TUN PE=2 SV=1
sp|Q59Q79|ALG1_CANAL	1.57e-92	35	448	45	444	Chitobiosyldiphosphodolichol beta-mannosyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ALG1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999986	0.000056

TMHMM  Annotations     

Start	End
4	23
111	133