CAZyme Information: ASPCADRAFT_206490-t33_1-p1

Basic Information

• Species: Aspergillus carbonarius
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus carbonarius
• CAZyme ID: ASPCADRAFT_206490-t33_1-p1
• CAZy Family: GH28
• CAZyme Description: glycosyltransferase family 66 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
742	KV907498|CGC8	83209.46	8.9535

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AcarbonariusITEM5010	11735	602072	260	11475

• Gene Location: location=KV907498:311225-313849(-)

Full Sequence      

MAESPLDVLLKGNSGRTTRGLLRIIILATIAAAAVSSRLFSVIRFESIIHEFDPWFNFRA
TKYLVQHGFESFWDWFDDRTWHPLGRVTGGTLYPGLMVTSGVIYHVLRFLTIPVDIRNIC
VLLAPGFSGLTALAMYFLTCEMATSPSAGLLAAAFMGIVPGYISRSVAGSYDNEAIAIFL
LVFTFFLWIKAVKNGSIMWGALAALFYGYMVSAWGGYVFITNLIPLHVFVLLCMGRYSSR
IYISYTTWYALGTLASMQIPFVGFLPIRNSDHMSALGVFGLIQLVAFADFVRGFIPGKHF
QRLLTTMIVVVFGIAFVGLVVLTVSGVIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPT
AWPAFFFDLNFLIWLFPAGVYMCFRDLKDEHVFVIIYSVLASYFAGVMVRLMLTLTPIVC
VAAALALSTILDTYVFATNGPNPRAKANEDTSSEGLRSARKPDVGITSYLSKAVMTSSVV
VYLLLFVAHCTWVTSNAYSSPSVVLASRMPDGSQHIIDDYREAYYWLRQNTAHNAKIMSW
WDYGYQIGGMADRPTLVDNNTWNNTHIATVGKAMSSREEVSYPILRQHDVDYVLVVFGGL
LGYSGDDINKFLWMVRIAEGIWPDEVKERDFFTARGEYRVDDGATPTMRNSLMYKMSYYN
FNSLFQPGQAVDRVRGSRLPAEGPQLSTLEEAFTSENWIIRIYKVKDLDNLGRDHNTAVA
FDKGNKRKRATKRKGPRVLRTE

Enzyme Prediction     

EC	2.4.99.18:21

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT66	23	664	6.1e-194	0.8513708513708513

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
396873	STT3	4.67e-150	35	499	15	478	Oligosaccharyl transferase STT3 subunit. This family consists of the oligosaccharyl transferase STT3 subunit and related proteins. The STT3 subunit is part of the oligosaccharyl transferase (OTase) complex of proteins and is required for its activity. In eukaryotes, OTase transfers a lipid-linked core-oligosaccharide to selected asparagine residues in the ER. In the archaea STT3 occurs alone, rather than in an OTase complex, and is required for N-glycosylation of asparagines.
224206	Stt3	1.86e-115	35	735	32	762	Asparagine N-glycosylation enzyme, membrane subunit Stt3 [Posttranslational modification, protein turnover, chaperones].
275016	archaeo_STT3	6.85e-31	51	554	32	547	oligosaccharyl transferase, archaeosortase A system-associated. Members of this protein family occur, one to three members per genome, in the same species of Euryarchaeota as contain the predicted protein-sorting enzyme archaeosortase (TIGR04125) and its cognate protein-sorting signal PGF-CTERM (TIGR04126).
404170	PMT_2	0.002	116	211	24	112	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CAS97683.1|GT66	0.0	1	742	1	741
CAK42956.1|GT66	0.0	1	742	1	741
BCS03802.1|GT66	0.0	1	742	1	741
BCS15416.1|GT66	0.0	1	742	1	741
UDD65301.1|GT66	0.0	1	742	1	742

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6C26_A	0.0	21	713	15	690	The Cryo-EM structure of a eukaryotic oligosaccharyl transferase complex [Saccharomyces cerevisiae S288C],6EZN_F Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex [Saccharomyces cerevisiae S288C],7OCI_F Chain F, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 [Saccharomyces cerevisiae S288C]
6S7T_A	1.89e-286	33	710	83	777	Cryo-EM structure of human oligosaccharyltransferase complex OST-B [Homo sapiens]
6FTG_5	4.19e-265	21	712	17	701	Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes [Canis lupus familiaris],6FTI_5 Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome [Canis lupus familiaris]
6S7O_A	8.41e-265	21	712	17	701	Cryo-EM structure of human oligosaccharyltransferase complex OST-A [Homo sapiens]
6FTJ_5	3.27e-262	21	707	17	696	Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome [Canis lupus familiaris]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O94335|STT3_SCHPO	0.0	22	728	24	738	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=stt3 PE=3 SV=1
sp|P39007|STT3_YEAST	0.0	21	713	15	690	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=STT3 PE=1 SV=2
sp|Q3TDQ1|STT3B_MOUSE	7.63e-287	33	710	80	774	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Mus musculus OX=10090 GN=Stt3b PE=1 SV=2
sp|E2RG47|STT3B_CANLF	6.86e-286	33	710	83	777	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Canis lupus familiaris OX=9615 GN=STT3B PE=1 SV=1
sp|Q8TCJ2|STT3B_HUMAN	9.72e-286	33	710	83	777	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B OS=Homo sapiens OX=9606 GN=STT3B PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000014	0.000001

TMHMM  Annotations     

Start	End
20	42
90	112
119	138
148	168
175	192
212	234
241	263
273	295
308	330
362	384
391	413
418	437
478	500