CAZyme Information: ASPBRDRAFT_114314-t33_1-p1

Basic Information

• Species: Aspergillus brasiliensis
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus brasiliensis
• CAZyme ID: ASPBRDRAFT_114314-t33_1-p1
• CAZy Family: AA1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
931		103924.19	4.7838

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AbrasiliensisCBS101740	13263	767769	274	12989

• Gene Location: location=KV878679:3385320-3388337(-)

Full Sequence      

MRYGIGFAAALLAPALPIVWGQHVRDLSNEHWTLSSRALNRTVPAQFPSQVHLDLLRAGV
IGEYHGLNDFNLRWIAAANWTYTSQPIKGLLDDYESTWLVFDGLDTFATISFCGQQIAST
DNQFRQYAFDVSTALGSCKGDPVLSINFGSAPNIVDAIAQDPNSQKWSDDVQLTYEYPNR
WFMRKEQSDFGWDWGPAFAPAGPWKPAYVVQLDKKESVYVLNTDLDIYRKGQINYLPPDQ
SQPWVVNASIDILGPLPAKPTMSIEVRDAHSGIILTSRALNNVTVSGGAVTGVTVLDGLT
PKLWWPQGLGDQNLYNVSITVNSSGNQTLATVAKRTGFRTIFLNQRNITEAQRAQGIAPG
ANWHFEINGHEFYAKGSNLIPPDSFWTRVTEEKMSQLFDAVVIGNQNMLRVWSSGAYLHD
YIYDLADEKGILLWSEFEFSDALYPSDDAFLENVAAEIVYNVRRVNHHPSLALWAGGNEI
ETLMLPRVKAADPSSYAWYVGEYEKMYISLFLPLVYENSRSISYSPSSTTEGYLYVDLSA
PVPMAERYDNTTSGSYYGDTDHYDYDTSVAFDYGSYPVGRFANEFGFHSMPSLQTWQQAV
DTEDLYFNSSVVMLRNHHDPAGGLMTDNYANSANGMGEMTMGVISYYPIPSKSDHISNFS
AWCHATQLFQADMYKNQIQFYRRGSGMPERQLGSLYWQLEDIWQAPSWAGIEYGGRWKVL
HHVARDIYQPVIVSPFWNYTTGSLDVYVTSDLWSPAAGTVDLTWLDLSGNPIAGNAGTPK
SVPFAVGGLNTTRVYGTNVSSLRLSDTKNAVLLLSLSAHGRLPNSDQATNFTHENHATLA
WPKDLKIVDPGIKLGYNAKKTSVTVEATSGVSLYTWLDYPEGVVGYFEENAFVLAPNETK
EIKFNVLEDTTNGAWARNITVQSLWDQKVQS

Enzyme Prediction     

EC	3.2.1.25:4

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	29	770	1.1e-74	0.6555851063829787

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
225789	LacZ	6.18e-49	24	735	12	636	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
407625	Ig_mannosidase	2.20e-18	849	926	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
236673	ebgA	2.04e-07	239	504	222	479	cryptic beta-D-galactosidase subunit alpha; Reviewed
407658	Mannosidase_ig	4.54e-07	742	844	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
395572	Glyco_hydro_2	8.45e-07	219	339	6	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS16350.1|GH2	0.0	1	931	1	932
BCS04781.1|GH2	0.0	1	931	1	932
CAK96951.1|GH2|3.2.1.25	0.0	1	930	1	930
A2QWU9|GH2	0.0	1	930	1	930
CAB63902.1|GH2|3.2.1.25	0.0	1	930	1	930

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CVU_A	0.0	24	926	30	938	Structure of Fungal beta-mannosidase from Glycoside Hydrolase Family 2 of Trichoderma harzianum [Trichoderma harzianum],4UOJ_A Chain A, BETA-MANNOSIDASE GH2 [Trichoderma harzianum],4UOJ_B Chain B, BETA-MANNOSIDASE GH2 [Trichoderma harzianum]
6DDT_A	2.07e-107	32	752	18	699	mouse beta-mannosidase (MANBA) [Mus musculus],6DDU_A mouse beta-mannosidase bound to beta-D-mannose (MANBA) [Mus musculus]
2VJX_A	4.46e-79	32	902	15	814	Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	4.61e-79	32	902	17	816	Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	4.69e-79	32	902	17	816	Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q4WMS9|MANBA_ASPFU	0.0	10	930	10	925	Beta-mannosidase A OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndA PE=3 SV=1
sp|B8NP78|MANBA_ASPFN	0.0	8	930	7	913	Beta-mannosidase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndA PE=3 SV=1
sp|O74168|MANBA_ASPAC	0.0	22	926	24	932	Beta-mannosidase A OS=Aspergillus aculeatus OX=5053 GN=mndA PE=1 SV=1
sp|Q2UN00|MANBA_ASPOR	0.0	8	930	7	913	Beta-mannosidase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=mndA PE=3 SV=1
sp|Q0CI48|MANBA_ASPTN	0.0	24	931	25	932	Beta-mannosidase A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.004427	0.995564	CS pos: 21-22. Pr: 0.9423

TMHMM  Annotations     

There is no transmembrane helices in ASPBRDRAFT_114314-t33_1-p1.