CAZyme Information: ASPACDRAFT_1875594-t33_1-p1

Basic Information

• Species: Aspergillus aculeatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus aculeatus
• CAZyme ID: ASPACDRAFT_1875594-t33_1-p1
• CAZy Family: CE1
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
663	KV878987|CGC4	70517.99	4.5379

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AaculeatusATCC16872	11165	690307	322	10843

• Gene Location: location=KV878987:654343-656561(-)

Full Sequence      

MSKFLAVSLLSVAVAAAPSLHPRLQNGLAQTPPMGWNTYNHYSCSPNETIVRSNAQALVD
LGLDSLGYRYVTTDCGWSVADRLADGSLTWNPELFPQGFPALGEYIHDLGLLFGVYGDAG
ILLCGSPPNNTGNLGHEEQDAQTFASWKVDSLKYDNCYSDAATNFPNVNYAPSTSPSSRY
ATMSQALAQLNRTVLFQVCDWGVDFPALWAPLLGHTWRIGNDIIPAWRTIYRILNQAVPQ
TDVAGPGQWPDLDMLEVGNGVFSLAEEQTHFSLWAILKTPLVIGAALRDEATAISESSLA
VLKQEDVIAFNQDALGTSASLRRRWSEEEYEVWSGPLSGGRTVAALVNWRNESRELTLDL
PDIGLQYAGSVKNIWEGSVAEGVRTSYTATVAGHGTMLLELGDTIAEGVYPGDLFVKTSG
TTTTFSPIYAVTTSKNYTLTITLSKPLSSPTAILVSTGSNTTTRLTIPPSITNFTTPLSL
SASPNATLTFHHPSTPTLPITSITLTPPQGTYAPSTAFTLSGTATLTTCGAGYCLPVGSK
LGYLSPSGSASTTIHASSAGTKYIEVAYINNDIAFDSSWDEGRNARNLTISVNGGAPVRL
EVPLSGRHSELFGPGLGWWDSATLGVSVDGWIVGGNEVVVGNVGGGEGVQSYGADLVGVR
VLD

Enzyme Prediction     

EC	3.2.1.22:2

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH27	132	378	3.8e-67	0.9694323144104804

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
269893	GH27	4.40e-118	32	313	1	271	glycosyl hydrolase family 27 (GH27). GH27 enzymes occur in eukaryotes, prokaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-N-acetylgalactosaminidase, and 3-alpha-isomalto-dextranase. All GH27 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. GH27 members are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively.
166449	PLN02808	1.03e-102	24	394	24	376	alpha-galactosidase
177874	PLN02229	1.05e-99	4	396	13	412	alpha-galactosidase
178295	PLN02692	2.10e-96	24	398	48	405	alpha-galactosidase
374582	Melibiase_2	3.77e-61	31	313	1	284	Alpha galactosidase A.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCR99189.1|CBM35|GH27	0.0	14	663	15	661
UDD59118.1|CBM35|GH27	0.0	13	662	13	657
QMW29010.1|CBM35|GH27	0.0	3	662	4	657
QMW41085.1|CBM35|GH27	0.0	3	662	4	657
QRD85275.1|CBM35|GH27	0.0	3	662	4	655

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1UAS_A	2.39e-85	25	394	2	352	Chain A, alpha-galactosidase [Oryza sativa]
6F4C_B	3.06e-82	24	394	1	353	Nicotiana benthamiana alpha-galactosidase [Nicotiana benthamiana]
3A5V_A	1.88e-73	26	403	3	392	Crystal structure of alpha-galactosidase I from Mortierella vinacea [Umbelopsis vinacea]
3LRK_A	2.56e-70	26	363	24	376	Chain A, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRL_A Chain A, Alpha-galactosidase 1 [Saccharomyces cerevisiae]
3LRM_A	3.67e-69	26	363	24	376	Chain A, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRM_B Chain B, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRM_C Chain C, Alpha-galactosidase 1 [Saccharomyces cerevisiae],3LRM_D Chain D, Alpha-galactosidase 1 [Saccharomyces cerevisiae]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A2R2S6|AGALD_ASPNC	0.0	14	663	14	660	Probable alpha-galactosidase D OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=aglD PE=3 SV=2
sp|B8N7Z0|AGALD_ASPFN	0.0	3	662	4	655	Probable alpha-galactosidase D OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=aglD PE=3 SV=1
sp|Q2UI87|AGALD_ASPOR	0.0	3	662	4	657	Probable alpha-galactosidase D OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=aglD PE=3 SV=2
sp|B0YEK2|AGALD_ASPFC	1.36e-313	7	663	5	648	Probable alpha-galactosidase D OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=aglD PE=3 SV=2
sp|A4DA70|AGALD_ASPFU	1.36e-313	7	663	5	648	Probable alpha-galactosidase D OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=aglD PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000272	0.999693	CS pos: 23-24. Pr: 0.9345

TMHMM  Annotations     

There is no transmembrane helices in ASPACDRAFT_1875594-t33_1-p1.