CAZyme Information: AO090701000274-T-p1

Basic Information

• Species: Aspergillus oryzae
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus oryzae
• CAZyme ID: AO090701000274-T-p1
• CAZy Family: GT32
• CAZyme Description: Beta-glucosidase-related glycosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
779		85095.21	4.7276

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AoryzaeRIB40	12364	510516	274	12090

• Gene Location: location=Chr5_A_oryzae_RIB40:1618591-1621549(+)

Full Sequence      

MVSGVFTKGVLLLGLLSGLALGQDEKPRYKDPSVPVEERVTDLLGRMTLEEKMSQLIQGD
ITNWMNETTGEFNLTGLEWSTKMRGGMFYVGYPVPWDYIADNVKKAQDYILQNTTLGIPA
IVQTESLHGFLIGNATIYNSPIGFACSFNPELIEKMARLIGQEASALGVNHVMGPVVDLA
RELRFGRVEETYGEDPFLAGEIGYHYTKGIQSHNISANVKHFVGFSQPEQGLNTAPVHGG
ERYLRTTWLPSFKRAIMDAGAWSIMSAYHSYDGIPAVADYHTLTEILREEWGYKYWVTSD
AGASDRVCTAFKLCRADPIDKEAVTLAILPAGNDVEMGGGSYNFETIIDLVNAGKLDIEI
VNTAVSRVLRAKFEMGLFENPYNAAPASEWNKLIHTQEAVDLARELDRESIVLLENHDNA
LPLKKSGSIAVIGPMAHGFMNYGDYVVYESQYRGVTPLDGIKAAVGDKATINYAQGCERW
SNDQSGFAEAVEAAKKSDVAVVVVGTWSRDQKELWAGLNATTGEHVDVNSLSLVGAQAPL
IKAIIDTGVPTVVVLSSGKPITEPWLSNNTAALVQQFYPSEQGGNALADVLFGDYNPSGK
LSVSFPHSVGDLPIYYDYLNSAREIGDAGYIYSNGTLEFGHQYALGNPKAWYPFGYGKSY
SSFEYGAVKLDKTNVTEADTVTVSVDVKNTDATREGTEVVQVYVVDEVASVVVPNRLLKG
FKKVVIPAGQTKTVEIPLKVQDLGLWNVRMKYVVEPGAFGVLVGSSSEDIRGNATFYVQ

Enzyme Prediction     

EC	3.2.1.120:1	3.2.1.21:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	112	337	3e-60	0.9814814814814815

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185053	PRK15098	4.53e-148	32	776	27	762	beta-glucosidase BglX.
224389	BglX	4.89e-73	47	478	1	396	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
178629	PLN03080	7.65e-68	116	764	79	769	Probable beta-xylosidase; Provisional
396478	Glyco_hydro_3_C	9.19e-64	411	660	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
395747	Glyco_hydro_3	5.10e-52	48	371	1	316	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE62006.1|GH3|3.2.1.120	0.0	1	779	1	779
QMW32562.1|GH3	0.0	1	779	1	779
QMW44592.1|GH3	0.0	1	779	1	779
UDD57574.1|GH3	0.0	1	779	1	779
QRD84059.1|GH3	0.0	1	779	1	815

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7EAP_A	0.0	23	779	1	757	Chain A, Fn3_like domain-containing protein [Aspergillus oryzae RIB40]
5YOT_A	0.0	23	779	1	757	Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YOT_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_A Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40],5YQS_B Isoprimeverose-producing enzyme from Aspergillus oryzae in complex with isoprimeverose [Aspergillus oryzae RIB40]
5Z87_A	3.83e-113	2	771	12	778	Chain A, EmGH1 [Aurantiacibacter marinus],5Z87_B Chain B, EmGH1 [Aurantiacibacter marinus]
5XXL_A	2.83e-107	43	771	17	745	Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXL_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],5XXM_A Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482],5XXM_B Crystal structure of GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with gluconolactone [Bacteroides thetaiotaomicron VPI-5482]
5XXN_A	5.53e-107	43	771	17	745	Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXN_B Crystal Structure of mutant (D286N) beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorose [Bacteroides thetaiotaomicron VPI-5482],5XXO_A Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482],5XXO_B Crystal structure of mutant (D286N) GH3 beta-glucosidase from Bacteroides thetaiotaomicron in complex with sophorotriose [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|T2KMH0|PLH24_FORAG	5.36e-124	113	768	60	711	Beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22130 PE=1 SV=1
sp|Q56078|BGLX_SALTY	7.65e-115	40	771	38	758	Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bglX PE=3 SV=2
sp|P33363|BGLX_ECOLI	2.23e-113	40	771	38	758	Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) OX=83333 GN=bglX PE=3 SV=2
sp|T2KMH9|PLH34_FORAG	6.72e-99	29	771	29	748	Putative beta-xylosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22230 PE=2 SV=1
sp|P14002|BGLB_ACET2	2.86e-93	116	770	35	660	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000197	0.999799	CS pos: 22-23. Pr: 0.9802

TMHMM  Annotations     

There is no transmembrane helices in AO090701000274-T-p1.