CAZyme Information: AO090138000086-T-p1

Basic Information

• Species: Aspergillus oryzae
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus oryzae
• CAZyme ID: AO090138000086-T-p1
• CAZy Family: GT25
• CAZyme Description: Has domain(s) with predicted DNA binding, RNA polymerase II transcription factor activity, sequence-specific DNA binding, polygalacturonase activity, zinc ion binding activity

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1392	Chr6_A_oryzae_RIB40|CGC4	155484.55	6.8116

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AoryzaeRIB40	12364	510516	274	12090

• Gene Location: location=Chr6_A_oryzae_RIB40:4016437-4020967(-)

Full Sequence      

MLNIMTRRVLSATLFILQCASALPALNSSIETYHFPEGLDRAHSYGIQVKQSTGGVHTVE
SYSAIVSEANTTSGKGIEHNTSFALFDFEGSVDLIVTYNNGPVKSAVIRPYSYGIKPTVK
GNTVSFSLDRPRNVVLQVNDDIFDTLQLFTNAIETDIPSQRDPNVVYFGPGIDNGPSSKN
GTLVIPSGKAVYLAPGAVVTSRLAFQNATSGSIQGRGVINPRSSGGILIEWSSNILVKDI
LILGAKGFSVTTGTSKNITMSGIRSMSSTGNGDGIDFFCSEDVLIDGVFLRNSDDNIALY
QHRWNYYGNSKNITVQNSSLWADWAHPINIGTHGNTDKPETMDGVTIRNIDILDHREPQM
WYQGCIAINPGDSNLIQNVHIEDVRVEDFRLGQLLNFRVMYNSKYNTSPGRGIRNVYVKD
LVYNGSHANPSLFLGYDKDRNISNVTFVNLQVNGKLIDDNMAKPSWYYTADFVPMVDWFH
SLLNPKLRRFFPMYRDLSWQEPLFFESTMEAPSRRVGILPKREIVNKSSHWCYAMIFTWT
PPDEAFGETPPLLPALLFSFHAGVKQTPQSCNGCPVGNWHSMRQHGRNGRSAQDQPPQQQ
QGQDTSKPEDDIPACQSCRKKKARCSREQPCSQCERFGVACVYDDRRLKPGLRAGAVDQL
YRRIDTLENMFLGQSILWKQVWEALHPNSAFPSAPDENLHNEESTGRQLAGARDAMKKSM
LQLAETKDTEGPSIESEHNGDGDCRSPKRRKVDLMSTPQQPSRNATDFDILNSDLVHPLV
EFYFTNIHHWIPILHVRRFREQIQFYKGRQKATYILHAIVALCSRFSDDSRLGSDAEKAE
LAEKCRQKVILSSMESFSVENLQALVIIAFDTIGRGRGPSSWSIVGGMARTVEQLQLSVE
EEHLSSHSQSGETLIRRMAFLKPSTSWREAEERRRVFWTVFLMDRFCSVSTGWNISLTSA
DVKRRLPCEGALWEQETEVRPPYFGISDARAASPHRPLLTESRMAADPKEQDCIGGFAYC
IEATESLALVTNFFLHHALDIRDADKAQLWLMRFKELDLRMVQWKLFLPPKWRDASVLNA
DGIMDPNLTLAHTTHNTAVILLHQGIAYPPLHWQSCPVKLPSTSSAETCLEAASEISTIG
QQFLLCSSILTNPQFSFCLFIAGRMLLTHSKYYGTPIPNSLDSLIASLFEISRRWAGPQN
VHDNLKDNLASGFAKRLVSAKDSFPTLSKPSLDIRQTAYSENTDHRAPPKRVETISTPSV
LERSSVVPNNTYAEQEPPLPIPDNHSDTSVGLAFPPLPLSFQQTLQSFTDVDPFGISLTD
RDQFNSQPSHVSIWNNATSPLYASAPIALDCASPEHPPTTLSPGQRISRYGAVEVDRAVV
MGENHAHLNHNS

Enzyme Prediction     

No EC number prediction in AO090138000086-T-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	198	455	2.9e-35	0.7538461538461538

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
213391	fungal_TF_MHR	3.92e-48	774	1196	4	392	fungal transcription factor regulatory middle homology region. This domain is present in the large family of fungal zinc cluster transcription factors that contain an N-terminal GAL4-like C6 zinc binuclear cluster DNA-binding domain. Examples of members of this large fungal group are the following Saccharomyces cerevisiae transcription factors, GAL4, STB5, DAL81, CAT8, RDR1, HAL9, PUT3, PPR1, ASG1, RSF2, PIP2, as well as the C-terminal domain of the Cep3, a subunit of the yeast centromere-binding factor 3. It has been suggested that this region plays a regulatory role.
397964	Fungal_trans	1.28e-17	780	977	1	195	Fungal specific transcription factor domain.
214903	Fungal_trans	1.12e-10	858	969	1	90	Fungal specific transcription factor domain. This domain is found in a number of fungal transcription factors including transcriptional activator xlnR, yeast regulatory protein GAL4, and other transcription proteins regulating a variety of cellular and metabolic processes.
214501	GAL4	1.79e-10	614	650	6	43	GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain. Gal4 is a positive regulator for the gene expression of the galactose- induced genes of S. cerevisiae. Is present only in fungi.
238023	GAL4	4.54e-09	614	644	5	36	GAL4-like Zn2Cys6 binuclear cluster DNA-binding domain; found in transcription regulators like GAL4. Domain consists of two helices organized around a Zn(2)Cys(6 )motif; Binds to sequences containing 2 DNA half sites comprised of 3-5 C/G combinations

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE64464.1|GH28	0.0	1	1392	1	1375
QRD81238.1|GH28	0.0	1	1392	1	1375
QMW38996.1|GH28	0.0	1	475	1	475
QMW26916.1|GH28	0.0	1	475	1	475
UDD54720.1|GH28	0.0	5	475	1	471

PDB Hits     

AO090138000086-T-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A0A3G1DJJ7|MFL3_PHOSM	6.97e-14	614	1103	762	1202	Squalestatin S1 biosynthesis transcriptional activator L3 OS=Phoma sp. (strain ATCC 20986 / MF5453) OX=1828523 GN=L3 PE=4 SV=1
sp|A0A162J452|CTNR_MONRU	3.92e-13	614	1107	23	458	Citrinin biosynthesis transcriptional activator mrl3 OS=Monascus ruber OX=89489 GN=ctnA PE=2 SV=1
sp|Q1ERI1|CTNR_MONPU	4.71e-13	614	1107	28	463	Citrinin biosynthesis transcriptional activator ctnR OS=Monascus purpureus OX=5098 GN=ctnR PE=3 SV=1
sp|G2TRN9|YAOB_SCHPO	5.04e-11	614	990	5	339	Uncharacterized transcriptional regulatory protein C11D3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC11D3.11c PE=1 SV=1
sp|A0A0M9ER64|DEP6_FUSLA	2.14e-10	614	984	16	394	Depudecin biosynthesis cluster-specific transcription activator DEP6 OS=Fusarium langsethiae OX=179993 GN=DEP6 PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.004506	0.995464	CS pos: 22-23. Pr: 0.9111

TMHMM  Annotations     

There is no transmembrane helices in AO090138000086-T-p1.