CAZyme Information: AO090113000019-T-p1

Basic Information

• Species: Aspergillus oryzae
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus oryzae
• CAZyme ID: AO090113000019-T-p1
• CAZy Family: GT1
• CAZyme Description: Has domain(s) with predicted catalytic activity, chitinase activity, hydrolase activity, hydrolyzing O-glycosyl compounds activity and role in carbohydrate metabolic process, chitin catabolic process

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
928	Chr5_A_oryzae_RIB40|CGC20	104135.24	5.1670

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AoryzaeRIB40	12364	510516	274	12090

• Gene Location: location=Chr5_A_oryzae_RIB40:4093715-4097070(+)

Full Sequence      

MTGRELVNITSLLTSIPAVGCQSNCNEPDKPSPGAKGDVRNKVIGYYESWRDGGSKCDTM
SPEEIPIEQLDQSTLAFVYIDPKDYHIIAMDEGPTASDLFARVANLKTRNPNAKIWVSIG
GWAFNDDGPYRSVFTKVAGDSKATQILAHNLMGFMDKYGFDGVDIDWEYPGADDRGGREE
DIANYPKMMSILKRYLQGGNIYSKKWGLSLTVPTSYWYLRWFDIKLLEKVVDEFNLMAYD
LHGRWDREDPIGPYVYAHTNLTEIDQALDLFWRNDIDSSKINLDLAVREYPSLIDIPLPL
HCSSCPKGPCTTTAGILSYSEIQRILSEGNIYPTYDEEAGVYYANYGNGGANWVSFDDTI
SFNAKIELANKYGLGSVLIWAVDQDDQYYHAMRGVTGKDIEAIPMDRGGFGAFTVDQCYI
TDCGKSCNPGDITMTKLNDESGRGCGGKNHNARSYCCPAGNAPDSSTWEVSMVFDDYGDS
KSDARTEGRKSDCILADYEKSCPSDKPQEMTSPSLYVKHECIFQREGRKLTSPFMNRNMT
LISVGGTGKDAIAMTMHAPRARLSSCDGMDGMRAFLNGILVATKPVNRHFAAHYLSAMDL
PSSHYTTTFSEVFDGSQDETSSKVSGTDPNKKAFTWMAMVGEAEDVQSFDKRDGSHLELF
DCPDTHEEDFGVQQAKAVCVGGTDEENNCEDIFHGGVEGTVVRLPAHCGPDAYVRAVRFQ
RSSNFTLPGHLRKRHPTAYKVYDFHYDYDFHNLRRDGGEVYFHADLSNHPSFWDEIVKAD
HNSPVKRSAKNWRQLDRRYWSESREDWLKRFNALLVKGHTGLEKHYEFNQCLVESRAVCG
NGSAEVKASVYGEFNTTMDFGMSLIGRLRNFGFSEAYSYFNQEAFSMRMGAAFTARARMY
FDSGWKSIGSFDQRICVSRGDNRDDCLS

Enzyme Prediction     

No EC number prediction in AO090113000019-T-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	40	391	8.3e-57	0.9594594594594594

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
214753	Glyco_18	1.22e-60	42	385	1	334	Glyco_18 domain.
395573	Glyco_hydro_18	7.91e-56	42	385	1	307	Glycosyl hydrolases family 18.
119351	GH18_chitolectin_chitotriosidase	1.25e-50	43	386	1	342	This conserved domain family includes a large number of catalytically inactive chitinase-like lectins (chitolectins) including YKL-39, YKL-40 (HCGP39), YM1, oviductin, and AMCase (acidic mammalian chitinase), as well as catalytically active chitotriosidases. The conserved domain is an eight-stranded alpha/beta barrel fold belonging to the family 18 glycosyl hydrolases. The fold has a pronounced active-site cleft at the C-terminal end of the beta-barrel. The chitolectins lack a key active site glutamate (the proton donor required for hydrolytic activity) but retain highly conserved residues involved in oligosaccharide binding. Chitotriosidase is a chitinolytic enzyme expressed in maturing macrophages, which suggests that it plays a part in antimicrobial defense. Chitotriosidase hydrolyzes chitotriose, as well as colloidal chitin to yield chitobiose and is therefore considered an exochitinase. Chitotriosidase occurs in two major forms, the large form being converted to the small form by either RNA or post-translational processing. Although the small form, containing the chitinase domain alone, is sufficient for the chitinolytic activity, the additional C-terminal chitin-binding domain of the large form plays a role in processing colloidal chitin. The chitotriosidase gene is nonessential in humans, as about 35% of the population are heterozygous and 6% homozygous for an inactivated form of the gene. HCGP39 is a 39-kDa human cartilage glycoprotein thought to play a role in connective tissue remodeling and defense against pathogens.
119357	GH18_zymocin_alpha	6.45e-45	42	383	1	343	Zymocin, alpha subunit. Zymocin is a heterotrimeric enzyme that inhibits yeast cell cycle progression. The zymocin alpha subunit has a chitinase activity that is essential for holoenzyme action from the cell exterior while the gamma subunit contains the intracellular toxin responsible for G1 phase cell cycle arrest. The zymocin alpha and beta subunits are thought to act from the cell's exterior by docking to the cell wall-associated chitin, thus mediating gamma-toxin translocation. The alpha subunit has an eight-stranded TIM barrel fold similar to that of family 18 glycosyl hydrolases such as hevamine, chitolectin, and chitobiase.
119365	GH18_chitinase	6.58e-42	43	385	1	322	The GH18 (glycosyl hydrolases, family 18) type II chitinases hydrolyze chitin, an abundant polymer of N-acetylglucosamine and have been identified in bacteria, fungi, insects, plants, viruses, and protozoan parasites. The structure of this domain is an eight-stranded alpha/beta barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE63084.1|GH18	0.0	1	928	1	928
BCR84639.1|CBM18|GH18	0.0	27	912	146	1084
UKZ63161.1|CBM18|GH18	2.85e-176	33	904	138	1098
UKZ63160.1|CBM18|GH18	7.14e-176	33	904	138	1098
UKZ52873.1|CBM18|GH18	9.94e-170	17	903	146	1093

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5WUP_A	1.16e-32	42	386	94	439	Crystal structure of a insect group III chitinase (CAD1) from Ostrinia furnacalis [Ostrinia furnacalis],5WV9_A Crystal structure of a insect group III chitinase complex with (GlcNAc)6 (CAD1-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]
5WUS_A	1.53e-32	42	386	3	343	Crystal structure of a insect group III chitinase (CAD2) from Ostrinia furnacalis [Ostrinia furnacalis],5WVH_A Crystal structure of an insect group III chitinase complex with (GlcNAc)6 (CAD2-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]
5WV8_A	1.23e-31	42	386	94	439	Crystal structure of a mutant insect group III chitinase (CAD1-E217L) from Ostrinia furnacalis [Ostrinia furnacalis],5WVB_A Crystal structure of a mutant insect group III chitinase complex with (GlcNAc)6 (CAD1-E217L-(GlcNAc)6) from Ostrinia furnacalis [Ostrinia furnacalis]
5WVF_A	1.64e-31	42	386	3	343	Crystal structure of a mutant insect group III chitinase (CAD2-E647L) from Ostrinia furnacalis [Ostrinia furnacalis],5WVG_A Crystal structure of a mutant insect group III chitinase complex with (GlcNAc)5 (CAD1-E647L-(GlcNAc)5) from Ostrinia furnacalis [Ostrinia furnacalis]
6JM7_A	2.21e-29	43	394	6	357	Crystal structure of Ostrinia furnacalis Group IV chitinase [Ostrinia furnacalis],6JMB_A Chain A, ofchtiv-allosamidin [Ostrinia furnacalis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P09805|KTXA_KLULA	3.61e-28	4	383	348	710	Killer toxin subunits alpha/beta OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 PE=1 SV=1
sp|P36362|CHIT_MANSE	6.27e-26	38	415	20	400	Endochitinase OS=Manduca sexta OX=7130 PE=2 SV=1
sp|Q6RY07|CHIA_RAT	9.42e-26	43	386	24	366	Acidic mammalian chitinase OS=Rattus norvegicus OX=10116 GN=Chia PE=2 SV=1
sp|Q15782|CH3L2_HUMAN	1.03e-25	36	386	22	366	Chitinase-3-like protein 2 OS=Homo sapiens OX=9606 GN=CHI3L2 PE=1 SV=1
sp|Q91XA9|CHIA_MOUSE	1.26e-25	43	386	24	366	Acidic mammalian chitinase OS=Mus musculus OX=10090 GN=Chia PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.559277	0.440732

TMHMM  Annotations     

There is no transmembrane helices in AO090113000019-T-p1.