CAZyme Information: AO090011000715-T-p1

Basic Information

• Species: Aspergillus oryzae
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus oryzae
• CAZyme ID: AO090011000715-T-p1
• CAZy Family: GH18
• CAZyme Description: Ortholog(s) have cellulase activity, cellulose binding activity and role in xyloglucan catabolic process

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
490	Chr7_A_oryzae_RIB40|CGC1	51667.64	4.4813

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AoryzaeRIB40	12364	510516	274	12090

• Gene Location: location=Chr7_A_oryzae_RIB40:1828960-1830646(-)

Full Sequence      

MRIGNLIVAASAASLVHAYPTRDIKKRGSGFTWVGVSESGAEFGSSIPGTLGTDYTWPDT
SKIQVLRDDGMNVFRIPFLMERLAPNQMTGSLDATYLKDLKSTVQAVTDSGAYVVLDPHN
YGRYSGSIISSTSDFKTFWKTVAGEFASNEKVIFDTNNEYHDMEQSLVLSLNQAAIDGIR
AAGATTQYIFVEGNSYSGAWKWADTNDNLSQLTDPQDKIVYEMHQYLDSDGSGTSETCAS
STIGKERLQTATEWLKTNNKKGFLGEFAGGVNEQCEQAVEGLLSYMSDNSDVWMGAEWWS
AGPWWGSYMYSMEPTDGTAYSTYLPILKKYFVDGAGASTSSSATSAAPSTAAASTSTSVS
ASTSSASSTTISAVESSSTSSVAEAPSTTSGVVTATPTPSHPAPQPTSNSSSASSGAPTS
SAPTTLATSPACGYQTTVTVTASRSTAAPSSSAGAVAHYYQCGGINYSGPTTCESGYTCV
KQNPYYSQCL

Enzyme Prediction     

EC	3.2.1.4:38

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH5	33	309	4.4e-117	0.9964539007092199

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395098	Cellulase	1.19e-74	36	302	1	272	Cellulase (glycosyl hydrolase family 5).
225344	BglC	2.10e-24	31	331	45	391	Aryl-phospho-beta-D-glucosidase BglC, GH1 family [Carbohydrate transport and metabolism].
197593	fCBD	3.28e-15	457	490	1	34	Fungal-type cellulose-binding domain. Small four-cysteine cellulose-binding domain of fungi
395595	CBM_1	7.47e-14	458	486	1	29	Fungal cellulose binding domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE65168.1|CBM1|GH5_5	2.53e-295	1	490	1	490
QMW35398.1|CBM1|GH5_5	4.18e-294	1	490	1	490
QMW47460.1|CBM1|GH5_5	4.18e-294	1	490	1	490
QRD91813.1|CBM1|GH5_5	4.18e-294	1	490	1	490
UDD64503.1|CBM1|GH5_5	2.41e-293	1	490	1	490

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1GZJ_A	3.28e-156	31	330	3	303	Structure of Thermoascus aurantiacus family 5 endoglucanase [Thermoascus aurantiacus],1GZJ_B Structure of Thermoascus aurantiacus family 5 endoglucanase [Thermoascus aurantiacus]
6T9F_A	3.81e-155	21	331	3	314	CRYSTAL STRUCTURE OF EN ENDOGLUCANASE S308P FROM PENICILLIUM VERRUCULOSUM [Talaromyces verruculosus],6T9F_B CRYSTAL STRUCTURE OF EN ENDOGLUCANASE S308P FROM PENICILLIUM VERRUCULOSUM [Talaromyces verruculosus],6T9F_C CRYSTAL STRUCTURE OF EN ENDOGLUCANASE S308P FROM PENICILLIUM VERRUCULOSUM [Talaromyces verruculosus],6T9F_D CRYSTAL STRUCTURE OF EN ENDOGLUCANASE S308P FROM PENICILLIUM VERRUCULOSUM [Talaromyces verruculosus]
5I77_A	4.98e-155	31	330	2	301	Crystal structure of a beta-1,4-endoglucanase from Aspergillus niger [Aspergillus niger]
1H1N_A	2.24e-154	31	330	4	304	Atomic resolution structure of the major endoglucanase from Thermoascus aurantiacus [Thermoascus aurantiacus],1H1N_B Atomic resolution structure of the major endoglucanase from Thermoascus aurantiacus [Thermoascus aurantiacus]
5I79_A	4.35e-154	31	330	4	303	Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar [Aspergillus niger],5I79_B Crystal structure of a beta-1,4-endoglucanase mutant from Aspergillus niger in complex with sugar [Aspergillus niger]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q96WQ8|EGLB_ASPKW	6.02e-158	5	330	6	331	Probable endo-beta-1,4-glucanase B OS=Aspergillus kawachii (strain NBRC 4308) OX=1033177 GN=eglB PE=3 SV=1
sp|Q2UPQ4|EGLB_ASPOR	5.06e-157	1	330	1	331	Probable endo-beta-1,4-glucanase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=eglB PE=3 SV=1
sp|B8MW97|EGLB_ASPFN	5.06e-157	1	330	1	331	probable endo-beta-1,4-glucanase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=eglB PE=3 SV=1
sp|A1DME8|EGLB_NEOFI	8.26e-155	1	330	1	327	Probable endo-beta-1,4-glucanase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=eglB PE=3 SV=1
sp|O74706|EGLB_ASPNG	1.45e-153	5	330	6	330	Endo-beta-1,4-glucanase B OS=Aspergillus niger OX=5061 GN=eglB PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000320	0.999660	CS pos: 27-28. Pr: 0.6835

TMHMM  Annotations     

There is no transmembrane helices in AO090011000715-T-p1.