CAZyme Information: AN9340-T-p1

Basic Information

• Species: Aspergillus nidulans
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus nidulans
• CAZyme ID: AN9340-T-p1
• CAZy Family: GT58
• CAZyme Description: Alpha,alpha-trehalase with a role in trehalose hydrolysis

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1054		115110.83	4.7793

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AnidulansFGSCA4	10988	227321	276	10712

• Gene Location: location=ChrVIII_A_nidulans_FGSC_A4:333424-336693(-)

Full Sequence      

MRFKSVFTLLPLLAQLPSGGASLPNNHGRVENCVRNHDGIHKFRHSNNTYQSMFPGVTWD
EDQWVLTTSSLDQGHYQSRGSVANGYIGISVSSVGPFFELDLPVAGDVINGWPLYSRRQS
FATISGFFDIQAETNGSNFPWMNQYGGESVISGVPHWSGLILDLGDDDYLDSTVDNVTLS
DFKSSYDFKAGVLSWSYTWTPAGDKGSYAITYRLFANKLNVNQAVVDMEITPSQDGHATI
VNVLDGYSAVRTDFVESQEDDGAIYSAVRPWGIPDVSAYFYANITGSKHVDLSSRRLIHG
KPYVSANESSIAQAADVNFVANEKVRITKFVGAASTDAFPDPQATAKRAVSEALDAGYQR
SLRSHVQEWASIMHEDSVDRYVNPTTGKLPDDDNIINSAIIAVANTYYLLQNTVGKNAIR
AAQDAPLNVNSFSVGGLVSDSYAGLVFWDADVWMQPGLVASHPEAAQAVTNYRTKLYPQA
KKNIETTYTGSKNATYIDPSAAIYPWTSGRFGNCTGTGACWDYQYHLNGDIGLSLIYQWV
VSGDTNTFREKHFPIYDSVAALYGSIVERNGSYWTLTNMTDPDEYANHIDAGGFTMPMIS
ETLEYANQFRQQFGLEPNETWTEISENVLVLRENGVTLEYTTMNGTAAVKQADIVLVTYP
LVYDNYTAETALTDLDYYANRQSADGPAMTWAIFSIAAGAVSPSGCSAYTYHQYSYAPYA
RAPFFQLSEQMLDNASINGGTHPAYPFLTGHGGANQVVLFGYLGLRLLPDDAIHIEPNLP
PQIPYVKYRTFYWRGWPISAQSNYTHTVLQRSQSAPLDTADRRFANTSIPVFVGLADNAT
LHHLPPHGPLTVRNREIGTINTIEDNLIQCSPVSSTDAFEQGQFPISVVDGATSTRWQPS
SSNASAVTVNLGSTTGRSVQTVASGFHFDWAAAPPVNASVIFHDTPLSDPVAALSSPGPH
VRIVANLTNIEQSGPYDPEATDLNEIKIPVGNTTRIELAQEVPVGRYATLVISGNQALAQ
ADGEDHVGATVAEWAILGPKSGSPRRRIQPVPLL

Enzyme Prediction     

EC	3.2.1.28:5	-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH65	405	767	7.7e-80	0.9838709677419355

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224471	ATH1	8.20e-50	60	795	7	726	Trehalose and maltose hydrolase (possible phosphorylase) [Carbohydrate transport and metabolism].
397616	Glyco_hydro_65N	1.07e-36	72	336	9	240	Glycosyl hydrolase family 65, N-terminal domain. This family of glycosyl hydrolases contains vacuolar acid trehalase and maltose phosphorylase.Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta-D-glucose-1-phosphate and glucose. This domain is believed to be essential for catalytic activity although its precise function remains unknown.
237517	PRK13807	1.51e-07	178	627	105	538	maltose phosphorylase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAB57642.1|GH65|3.2.1.28	0.0	1	1054	1	1054
EAA66407.1|GH65|3.2.1.28	0.0	9	1054	1	1046
BCS25489.1|GH65	0.0	1	1054	36	1088
UDD58012.1|GH65	0.0	9	1042	12	1048
QRD84477.1|GH65	0.0	9	1042	8	1044

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7FE3_A	4.48e-41	61	782	21	657	Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE3_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_A Chain A, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_B Chain B, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101],7FE4_C Chain C, Candidate alpha glycoside phosphorylase Glycoside hydrolase family 65 [Flavobacterium johnsoniae UW101]
3WIQ_A	5.36e-15	431	795	326	722	Crystal structure of kojibiose phosphorylase complexed with kojibiose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_A Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_B Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_C Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903],3WIR_D Crystal structure of kojibiose phosphorylase complexed with glucose [Caldicellulosiruptor saccharolyticus DSM 8903]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P78617|TREA_EMENI	0.0	1	1054	1	1054	Acid trehalase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=treA PE=3 SV=1
sp|D4B5H9|ATC1_ARTBC	0.0	50	1037	53	1051	Cell wall acid trehalase ARB_03719 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_03719 PE=1 SV=1
sp|Q5AAU5|ATC1_CANAL	6.78e-110	62	940	107	964	Cell wall acid trehalase ATC1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=ATC1 PE=1 SV=2
sp|M1RNJ8|ATH1_CANGB	1.35e-105	18	766	114	862	Periplasmic/secreted acid trehalase ATH1 OS=Candida glabrata OX=5478 GN=ATH1 PE=1 SV=1
sp|N1P212|ATH1_YEASC	2.98e-99	52	899	119	996	Periplasmic acid trehalase ATC1 OS=Saccharomyces cerevisiae (strain CEN.PK113-7D) OX=889517 GN=CENPK1137D_1727 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000215	0.999729	CS pos: 21-22. Pr: 0.9485

TMHMM  Annotations     

There is no transmembrane helices in AN9340-T-p1.