CAZyme Information: AMAG_08841-t26_1-p1

Basic Information

• Species: Allomyces macrogynus
• Lineage: Blastocladiomycota; Blastocladiomycetes; ; Blastocladiaceae; Allomyces; Allomyces macrogynus
• CAZyme ID: AMAG_08841-t26_1-p1
• CAZy Family: GH37
• CAZyme Description: trehalose-phosphatase, variant

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
875		95380.77	7.2275

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AmacrogynusATCC38327	19333	578462	535	18798

• Gene Location: location=GG745343:216890-219770(-)

Full Sequence      

MSPTAAAIAPTSAPDVTPTPRRVIHVLHMLPIVAVRLDRVAASPAPSAPNSRRHSRAATA
SMNGSAPLASTAPTVEALTAALHHQHQRDATRLDGLVVPDTQGKWGFAPRRGHGALYAGI
QSLDGVDGMSCVYVGSLGQVVDVYGRPLVGETWTDAEWRELMSMLWVERKCVPVDLDDAT
ATAYYDGYCKADLWPILHYSLWDSATDGRQEKSHWDAYCRANAAYASTAADYFRAGDLVC
VHDYHLLLAPSHLRTALDESALIGFFLHTPFPSSEVFRCLPSRAHLLHGVLGASLIGAQT
YSYTRHLLSACTRILGLETTPTGIDLDDGRHVAVGTFPIGIDVARALHQANDPVVRARAA
ALRDQYRGRKLIVGRDKLDQIRGVHQKLLAFAQFLEMYPQWRDQVVLIQVTSPSAENGKG
STKGLEAKVSELVAQINGKYGSLEVMPVVHVHRSIEQSELYALLSAADVGLITSVRDGFN
TSSHHFIVAQHATQQAPLLLSEFTGTAGSLSSAMLVNPWDRAGVAAALHAALTLPDHDRA
MRHAQMYAHVSRHTAQAWAGAFTKALATAASCNAMAVPRKRTPLLPIPKCLAAYRSSKKR
LFALDFDGTLSPIVARPEDAKPARAVLDALRALTADPRNHVYIISGRDQATLDEWLGQHV
SNLGLSAEHGSFLKPPGADAWIDIGEMQDWAWKDEVLRIFQFHTERTPGSMIELKKSSVC
WHYRLADPAFGMFQAKECQNHLENAVLTKLPLDLLRGKKVLEVRPSYVNKGEIVGRLLHS
ASRAQPFDYVLCAGDCETDMDMFRRLSREFAGKPGAAFSVMIGPASKRTVADWHLPAPAD
LIGVLQTMGRMEEEEVDVDGVHEVLESGVAIAASS

Enzyme Prediction     

EC	3.1.3.12:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT20	110	567	1.9e-148	0.9221052631578948

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
184712	PRK14501	0.0	168	848	70	723	putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional
215556	PLN03064	6.23e-169	159	794	152	797	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional
340820	GT20_TPS	1.60e-163	97	567	11	463	trehalose-6-phosphate synthase. Trehalose-6-Phosphate Synthase (TPS, EC 2.4.1.15) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain.
215555	PLN03063	5.35e-161	120	803	42	717	alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional
395781	Glyco_transf_20	1.38e-159	103	567	20	469	Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex,.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CDS06611.1|GT20	4.95e-255	81	848	127	871
CDS13004.1|GT20	8.46e-245	105	848	46	775
AJH76892.1|GT20	4.53e-237	84	853	69	834
CDS07232.1|GT20	1.40e-225	92	848	81	824
CDS09548.1|GT20	2.57e-225	105	848	83	833

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HVO_A	1.55e-99	144	566	57	474	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_C Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVO_D Structure of Aspergillus fumigatus trehalose-6-phosphate synthase B in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
6JBI_A	5.49e-99	144	566	46	462	Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBI_B Structure of Tps1 apo structure [Pyricularia oryzae 70-15],6JBR_A Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_B Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_D Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_F Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_H Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_K Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_M Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBR_O Tps1/UDP/T6P complex [Pyricularia oryzae 70-15],6JBW_A Structure of Tps1/UDP complex [Pyricularia oryzae 70-15],6JBW_B Structure of Tps1/UDP complex [Pyricularia oryzae 70-15]
5HVM_A	2.39e-98	90	566	18	474	Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293],5HVM_B Structure of Aspergillus fumigatus trehalose-6-phosphate synthase A in complex with UDP and validoxylamine A [Aspergillus fumigatus Af293]
5DXF_A	1.21e-95	188	562	151	519	Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314],5DXF_B Structure of Candida albicans trehalose-6-phosphate phosphatase N-terminal domain [Candida albicans SC5314]
5HUT_A	8.52e-89	144	566	50	467	Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUT_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP-glucose [Candida albicans SC5314],5HUU_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HUU_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and glucose-6-phosphate [Candida albicans SC5314],5HVL_A Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314],5HVL_B Structure of Candida albicans trehalose-6-phosphate synthase in complex with UDP and validoxylamine A [Candida albicans SC5314]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P78875|TPP1_SCHPO	6.71e-164	188	850	163	814	Trehalose-phosphatase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tpp1 PE=1 SV=2
sp|P31688|TPS2_YEAST	2.95e-156	188	851	150	846	Trehalose-phosphatase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TPS2 PE=1 SV=3
sp|Q54K57|TPSA_DICDI	9.36e-156	90	841	22	727	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] A OS=Dictyostelium discoideum OX=44689 GN=tpsA PE=2 SV=1
sp|Q54NU9|TPSB_DICDI	1.66e-152	184	806	138	750	Alpha,alpha-trehalose-phosphate synthase [UDP-forming] B OS=Dictyostelium discoideum OX=44689 GN=tpsB PE=3 SV=1
sp|Q9P4D3|TPS2_ZYGRO	3.23e-152	187	851	154	853	Trehalose-phosphatase OS=Zygosaccharomyces rouxii OX=4956 GN=TPS2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000046	0.000002

TMHMM  Annotations     

There is no transmembrane helices in AMAG_08841-t26_1-p1.