CAZyme Information: AKAW_08892-t41_1-p1

Basic Information

• Species: Aspergillus luchuensis
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus luchuensis
• CAZyme ID: AKAW_08892-t41_1-p1
• CAZy Family: GH72|CBM43
• CAZyme Description: rhamnogalacturonase (RhgB)

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
561	DF126475|CGC2	58270.96	4.0003

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AluchuensisIFO4308	11755	1033177	267	11488

• Gene Location: location=DF126475:363952-365831(+)

Full Sequence      

MLLDKRSFLSFLGLAPIFAAAQLSGSVGPLTSASTKAATKTCNVLDYGAVADKSTDLGAP
LASAFADCKSGGLVYVPSGDYALSTWARLSGGEAWALQIDGIIYRTGTDGGNMIYIEHSS
DFELFSSTSEGAMQGLGYEFHADDNWSGPRLLRLYEVTDFSVHDFILVDSPSFHFSLDTC
TNGEIYNMAIRGGNHGGLDGIDVWSNNIWVHDVEVTNKDECVTVKSPSKNILIESIYCNW
SGGCGMGSFGSDTNVSDITYRNIYTWNSNNMMLIKSNGGSGFVENVLLENFIGHGNAYSL
DIDSYWASMSAVDGDGVQLSNITVKNWKGTEAYGAQRGPVKVICADGAPCYDITIEDFAM
WTEEGDSQWYSCESAYGSGFCLQDSDDHVSYSATTSTVDSAPSGYSATSMAADLTTDFGS
TVSIPIPTIPTSFYPGATPYSALMADSAFTAAASSLASHATVSSSSVSVAASVPSAVAPS
ESIPAATSAVVSSAAAVAPSPAVGAQEGSTTSAPSFAAPSGAGNSPQGPSGASGFGQKGQ
QGEQGGQGEQGEQGGQEVCYV

Enzyme Prediction     

EC	3.2.1.171:11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	64	375	2.2e-55	0.9661538461538461

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
395231	Glyco_hydro_28	1.14e-20	149	373	85	313	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.
215540	PLN03010	3.17e-19	43	386	48	397	polygalacturonase
215426	PLN02793	1.72e-16	149	404	177	437	Probable polygalacturonase
165802	PLN02155	1.40e-15	35	377	21	380	polygalacturonase
177865	PLN02218	2.57e-14	33	356	60	404	polygalacturonase ADPG

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS11707.1|GH28	0.0	1	561	1	561
BCR99404.1|GH28	0.0	1	561	1	561
CAK48512.1|GH28	0.0	1	529	1	529
CAA63912.1|GH28|3.2.1.171	0.0	1	529	1	529
BAE66335.1|GH28	8.13e-274	1	451	1	453

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1RMG_A	2.98e-233	22	443	1	420	Rhamnogalacturonase A From Aspergillus Aculeatus [Aspergillus aculeatus]
4C2L_A	7.99e-16	52	368	26	368	Crystal structure of endo-xylogalacturonan hydrolase from Aspergillus tubingensis [Aspergillus tubingensis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A2R3G4|RHGB_ASPNC	0.0	1	529	1	529	Probable rhamnogalacturonase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rhgB PE=3 SV=1
sp|P87161|RHGB_ASPNG	0.0	1	529	1	529	Rhamnogalacturonase B OS=Aspergillus niger OX=5061 GN=rhgB PE=1 SV=1
sp|Q2TWN8|RHGB_ASPOR	1.44e-274	1	451	1	453	Probable rhamnogalacturonase B OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rhgB PE=3 SV=1
sp|Q0CFC7|RHGB_ASPTN	2.33e-274	1	444	1	443	Probable rhamnogalacturonase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=rhgB PE=3 SV=1
sp|A1DED1|RHGB_NEOFI	3.93e-268	1	444	1	444	Probable rhamnogalacturonase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=rhgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.003411	0.996594	CS pos: 37-38. Pr: 0.2744

TMHMM  Annotations     

There is no transmembrane helices in AKAW_08892-t41_1-p1.