CAZyme Information: AGR57_10779T0-p1

Basic Information

• Species: Phanerochaete chrysosporium
• Lineage: Basidiomycota; Agaricomycetes; ; Phanerochaetaceae; Phanerochaete; Phanerochaete chrysosporium
• CAZyme ID: AGR57_10779T0-p1
• CAZy Family: AA3
• CAZyme Description: Glycoside Hydrolase Family 16 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
595	PchrRP-78_SC011|CGC4	65096.35	4.3741

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PchrysosporiumRP-78	13602	273507	0	13602

• Gene Location: location=PchrRP-78_SC011:837017-839390(+)

Full Sequence      

MSQPFQSPSTHNLLSPTGPAGAPSIRTVASNASLRSQSDSRYAIASEGSSSPVPPSISDK
FAMSPDPSSWGGDLSPNFREPDDELHNPDPKRDRKSDKGGTIFTSRGIANLGCLVILFGG
MGMLFAGYPLISHFTKKEQTTFGGFNLGGTNATGQVPKIPGNWGLVDLDTPKEAYTKVDY
VTGKTWELIFSDEFNVDGRTFYPGDDPYWEAVDLHYWQTNNMEWYDPAAITTRNGSLEIT
LSKKETHGLDYQGGMMSTWNKFCFTGGLIETSVVLPGANNIVGLWPAIWTMGNLGRAGYG
ASLEGMWPYTYDSCDVGTVANQTLNGLPIAATENGDPSNGGVLSFLPGQRLSRCTCPGES
HPGPVHSDGTYVGRSAPEIDIFEATMGGDPLQGQVSQSGQWGPFNAEYHWFNTSANLIIP
NTTLTELNPYMGGAFQQATSGLTFTNQEAYERTGNVYSVYGVQYQPGFDDAYIAWIADGK
MAWQLNQPGMAADPRVEIGSRPVPQEPMYIIANLGMSRNFGFVDLDHIIFPTTMKVDWIR
VYQDPDRKNIGCDPEDFPTAAYINEYIEAYTNPNLTTWRDDYGQPFPKNSFLGEC

Enzyme Prediction     

No EC number prediction in AGR57_10779T0-p1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	192	542	7.4e-136	0.9969325153374233

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
397841	SKN1	0.0	73	595	2	500	Beta-glucan synthesis-associated protein (SKN1). This family consists of the beta-glucan synthesis-associated proteins KRE6 and SKN1. Beta1,6-Glucan is a key component of the yeast cell wall, interconnecting cell wall proteins, beta1,3-glucan, and chitin. It has been postulated that the synthesis of beta1,6-glucan begins in the endoplasmic reticulum with the formation of protein-bound primer structures and that these primer structures are extended in the Golgi complex by two putative glucosyltransferases that are functionally redundant, Kre6 and Skn1. This is followed by maturation steps at the cell surface and by coupling to other cell wall macromolecules.
185689	GH16_fungal_KRE6_glucanase	1.92e-147	188	543	1	295	Saccharomyces cerevisiae KRE6 and related glucanses, member of glycosyl hydrolase family 16. KRE6 is a Saccharomyces cerevisiae glucanase that participates in the synthesis of beta-1,6-glucan, a major structural component of the cell wall. It is a golgi membrane protein required for normal beta-1,6-glucan levels in the cell wall. KRE6 is closely realted to laminarinase, a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
225182	BglS	4.71e-42	164	556	28	355	Beta-glucanase, GH16 family [Carbohydrate transport and metabolism].
185693	GH16_laminarinase_like	1.43e-19	190	542	1	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
185683	Glyco_hydrolase_16	3.16e-05	192	301	1	99	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRV94223.1|GH16_2	1.18e-265	57	595	68	608
QRW08448.1|GH16_2	9.58e-265	57	595	68	608
QRV79586.1|GH16_2	9.58e-265	57	595	68	608
QRW19070.1|GH16_2	4.83e-261	56	595	64	602
QRV91234.1|GH16_2	5.01e-261	56	595	83	603

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6JH5_A	8.02e-07	184	304	2	116	Structure of Marine bacterial laminarinase [Aquimarina]
6JHJ_A	1.08e-06	184	301	2	113	Structure of Marine bacterial laminarinase mutant-E135A [Aquimarina],6JIA_A Marine bacterial laminarinase mutant E135A complex with laminaritetraose [Aquimarina sp.],6M6P_A Structure of Marine bacterial laminarinase mutant E135A in complex with 1,3-beta-cellotriosyl-glucose [Aquimarina sp.]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P87024|SKN1_CANAX	1.79e-126	74	593	240	736	Beta-glucan synthesis-associated protein SKN1 OS=Candida albicans OX=5476 GN=SKN1 PE=2 SV=1
sp|P32486|KRE6_YEAST	3.15e-126	58	595	189	711	Beta-glucan synthesis-associated protein KRE6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=KRE6 PE=1 SV=2
sp|O13941|YEPB_SCHPO	6.62e-124	73	595	123	627	Uncharacterized beta-glucan synthesis-associated protein C23H3.11c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC23H3.11c PE=3 SV=1
sp|P87023|KRE6_CANAX	3.05e-121	56	593	211	739	Beta-glucan synthesis-associated protein KRE6 OS=Candida albicans OX=5476 GN=KRE6 PE=2 SV=1
sp|P33336|SKN1_YEAST	5.62e-115	74	595	251	763	Beta-glucan synthesis-associated protein SKN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SKN1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000047	0.000000

TMHMM  Annotations     

Start	End
108	130