CAZyme Information: AGR57_10276T0-p1

Basic Information

• Species: Phanerochaete chrysosporium
• Lineage: Basidiomycota; Agaricomycetes; ; Phanerochaetaceae; Phanerochaete; Phanerochaete chrysosporium
• CAZyme ID: AGR57_10276T0-p1
• CAZy Family: AA14
• CAZyme Description: Class II peroxidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
314	PchrRP-78_SC010|CGC4	33506.99	7.0394

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_PchrysosporiumRP-78	13602	273507	0	13602

• Gene Location: location=PchrRP-78_SC010:1250082-1251701(+)

Full Sequence      

MHNLIKVIYILIIATVAAMAAPPSPNRVRCSAGREAGHPECCKWFDVREEIQDRLFNRSC
GADVRKVRPAWHDAIAFSHTGGLKSGGGADGSILAHPIELAYPANVGLSDIAERQRALAL
KHNVSFGDFIQFASAVGLSNCEGAPRLTFLAGRPNASRAADDGLVPAPWHSADTLLARMA
DAGFAPHELVALLAGHSVATQQTVDASVAGTPLDSTPRTFDNAFFVETQARGTVCPGAAL
HKGEALSSSPHELRLQSDGALARDPRTAPTWKRFAEDQVEMARQFANAMAKLSVVGQDAA
SLVDCTDVVPRWRM

Enzyme Prediction     

EC	1.11.1.16:9	1.11.1.13:5	1.11.1.-:1

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
AA2	44	298	2.7e-89	0.996078431372549

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
173826	ligninase	1.78e-132	28	311	1	289	Ligninase and other manganese-dependent fungal peroxidases. Ligninases and related extracellular fungal peroxidases belong to class II of the plant heme-dependent peroxidase superfamily. All members of the superfamily share a heme prosthetic group and catalyze a multistep oxidative reaction involving hydrogen peroxide as the electron acceptor. Class II peroxidases are fungal glycoproteins that have been implicated in the oxidative breakdown of lignin, the main cell wall component of woody plants. They contain four conserved disulphide bridges and two conserved calcium binding sites.
173823	plant_peroxidase_like	6.12e-40	49	293	1	254	Heme-dependent peroxidases similar to plant peroxidases. Along with animal peroxidases, these enzymes belong to a group of peroxidases containing a heme prosthetic group (ferriprotoporphyrin IX), which catalyzes a multistep oxidative reaction involving hydrogen peroxide as the electron acceptor. The plant peroxidase-like superfamily is found in all three kingdoms of life and carries out a variety of biosynthetic and degradative functions. Several sub-families can be identified. Class I includes intracellular peroxidases present in fungi, plants, archaea and bacteria, called catalase-peroxidases, that can exhibit both catalase and broad-spectrum peroxidase activities depending on the steady-state concentration of hydrogen peroxide. Catalase-peroxidases are typically comprised of two homologous domains that probably arose via a single gene duplication event. Class II includes ligninase and other extracellular fungal peroxidases, while class III is comprised of classic extracellular plant peroxidases, like horseradish peroxidase.
395089	peroxidase	2.18e-27	47	277	1	187	Peroxidase.
173825	ascorbate_peroxidase	9.05e-26	47	296	16	249	Ascorbate peroxidases and cytochrome C peroxidases. Ascorbate peroxidases are a subgroup of heme-dependent peroxidases of the plant superfamily that share a heme prosthetic group and catalyze a multistep oxidative reaction involving hydrogen peroxide as the electron acceptor. Along with related catalase-peroxidases, ascorbate peroxidases belong to class I of the plant superfamily. Ascorbate peroxidases are found in the chloroplasts and/or cytosol of algae and plants, where they have been shown to control the concentration of lethal hydrogen peroxide molecules. The yeast cytochrome c peroxidase is a divergent member of the family; it forms a complex with cytochrome c to catalyze the reduction of hydrogen peroxide to water.
173827	secretory_peroxidase	8.01e-19	124	296	94	282	Horseradish peroxidase and related secretory plant peroxidases. Secretory peroxidases belong to class III of the plant heme-dependent peroxidase superfamily. All members of the superfamily share a heme prosthetic group and catalyze a multistep oxidative reaction involving hydrogen peroxide as the electron acceptor. Class III peroxidases are found in the extracellular space or in the vacuole in plants where they have been implicated in hydrogen peroxide detoxification, auxin catabolism and lignin biosynthesis, and stress response. Class III peroxidases contain four conserved disulphide bridges and two conserved calcium binding sites.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AAU82081.1|AA2	4.83e-225	1	314	1	315
AAU82082.1|AA2	1.22e-218	1	313	1	314
AAU82084.1|AA2	6.30e-218	1	311	1	312
AZJ17948.1|AA2	4.18e-104	6	311	28	342
AFR51951.1|AA2	8.17e-98	18	310	26	323

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4BM1_A	2.11e-95	29	310	3	290	CRYSTAL STRUCTURE OF MANGANESE PEROXIDASE 4 FROM PLEUROTUS OSTREATUS - CRYSTAL FORM I [Pleurotus ostreatus],4BM1_B CRYSTAL STRUCTURE OF MANGANESE PEROXIDASE 4 FROM PLEUROTUS OSTREATUS - CRYSTAL FORM I [Pleurotus ostreatus],4BM2_A Crystal Structure Of Manganese Peroxidase 4 From Pleurotus Ostreatus - Crystal Form Ii [Pleurotus ostreatus],4BM3_A Crystal Structure Of Manganese Peroxidase 4 From Pleurotus Ostreatus - Crystal Form Iii [Pleurotus ostreatus],4BM4_A Crystal Structure Of Manganese Peroxidase 4 From Pleurotus Ostreatus - Crystal Form Iv [Pleurotus ostreatus]
2VKA_A	2.10e-90	30	310	3	283	Site-Directed Mutagenesis of the Catalytic Tryptophan Environment in Pleurotus eryngii Versatile Peroxidase [Pleurotus eryngii]
4FDQ_A	5.59e-90	30	310	3	283	The crystal structures of several mutants of pleurotus eryngii versatile peroxidase [Pleurotus eryngii]
4G05_A	5.95e-90	30	310	3	283	The crystal structures of several mutants of Pleurotus eryngii versatile peroxidase [Pleurotus eryngii]
3FMU_A	6.55e-90	30	310	3	283	Chain A, Versatile peroxidase VPL2 [Pleurotus eryngii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q9UR19|VPL1_PLEER	9.40e-91	17	310	20	313	Versatile peroxidase VPL1 OS=Pleurotus eryngii OX=5323 GN=vpl1 PE=1 SV=1
sp|O94753|VPL2_PLEER	2.66e-90	17	310	20	313	Versatile peroxidase VPL2 OS=Pleurotus eryngii OX=5323 GN=vpl2 PE=1 SV=1
sp|P28314|PER_COPCI	3.66e-88	16	310	14	317	Peroxidase OS=Coprinopsis cinerea OX=5346 GN=CIP1 PE=1 SV=2
sp|P28313|PER_ARTRA	7.56e-88	16	310	14	318	Peroxidase OS=Arthromyces ramosus OX=5451 PE=1 SV=3
sp|A8NK72|PER_COPC7	1.47e-87	16	310	14	317	Peroxidase OS=Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) OX=240176 GN=CIP1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000184	0.999800	CS pos: 20-21. Pr: 0.9739

TMHMM  Annotations     

Start	End
7	24