CAZyme Information: AEO58087.1

Basic Information

• Species: Thermothelomyces thermophilus
• Lineage: Ascomycota; Sordariomycetes; ; Chaetomiaceae; Thermothelomyces; Thermothelomyces thermophilus
• CAZyme ID: AEO58087.1
• CAZy Family: GH16
• CAZyme Description: glycoside hydrolase family 16 protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
431	CP003004|CGC14	47015.60	4.6016

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_TthermophilusATCC42464	9292	573729	195	9097

• Gene Location: location=CP003004:3592874-3594513(+)

Full Sequence      

MSPPLPLPPPRRRPPLADLLATNVTPLFFHLHLHLHHLLVLLLIIIVVGYAPRTARADCE
CGYLAAVHGGDSSNRRALFTDLLETDFARLAAGARDDDDHDDGDDDDAGPDASPIEGWAR
QAFNLTRERARGEYGELFAVENVGFVAAAAAKKGDAGLRLMVRAEIVDGMVPVAELDTQR
LDLMWGTFRAMMKVSSVKGTCAAFFWYFNDTQEIDMEFLSKDFNASNGSYPVNLVLQSRE
AAAAGHDASGTGGGHFVRALLPFDPTGDFHEYRIDYLPGRVTFYADGEPLADMEGAAVPS
SPGHLILQHWSNGNRYWSGGPPAEDASLLVSYVKAYFNSSTAQRRRDWEARCRDPTAPGA
VCEIPDVAPGNWSAAADWFFADHANMTNNQTASGQSEEASSSSPSTEPSWSIIIIVCWLW
LWLAAEFASAV

Enzyme Prediction     

No EC number prediction in AEO58087.1.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH16	149	336	6.5e-89	0.9635416666666666

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
185683	Glyco_hydrolase_16	3.96e-43	158	336	40	210	glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues.
185684	GH16_lichenase	4.27e-13	186	311	69	184	lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure.
395585	Glyco_hydro_16	5.46e-12	184	325	32	158	Glycosyl hydrolases family 16.
185693	GH16_laminarinase_like	4.71e-06	186	336	80	235	Laminarinase, member of the glycosyl hydrolase family 16. Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
178706	PLN03161	8.73e-06	199	390	85	255	Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEO58087.1|GH16_23	2.56e-288	1	431	1	431
VBB86764.1|GH16_23	2.48e-140	55	391	41	366
CDP32289.1|GH16_23	3.51e-140	55	391	41	366
QBZ62950.1|GH16_23	1.53e-118	55	402	41	369
UQC77992.1|GH16_23	9.18e-114	56	394	20	330

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CPN_A	1.04e-10	185	344	14	174	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]
1AXK_A	1.15e-09	185	373	14	183	Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis],1AXK_B Engineered Bacillus Bifunctional Enzyme Gluxyn-1 [Bacillus subtilis]
1MAC_A	1.24e-09	185	319	70	192	Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans],1MAC_B Crystal Structure And Site-Directed Mutagenesis Of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase [Paenibacillus macerans]
1BYH_A	1.27e-09	185	319	72	194	MOLECULAR AND ACTIVE-SITE STRUCTURE OF A BACILLUS (1-3,1-4)-BETA-GLUCANASE [synthetic construct],1GLH_A Cation Binding To A Bacillus (1,3-1,4)-Beta-Glucanase. Geometry, Affinity And Effect On Protein Stability [Paenibacillus macerans],2AYH_A Crystal And Molecular Structure At 1.6 Angstroms Resolution Of The Hybrid Bacillus Endo-1,3-1,4-Beta-D-Glucan 4- Glucanohydrolase H(A16-M) [hybrid]
1CPM_A	1.27e-09	185	319	14	136	NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS [Paenibacillus macerans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|P23904|GUB_PAEMA	8.83e-09	185	319	95	217	Beta-glucanase OS=Paenibacillus macerans OX=44252 PE=1 SV=2
sp|P45797|GUB_PAEPO	1.62e-08	185	319	96	218	Beta-glucanase OS=Paenibacillus polymyxa OX=1406 GN=gluB PE=3 SV=1
sp|P27051|GUB_BACLI	1.84e-07	185	334	101	242	Beta-glucanase OS=Bacillus licheniformis OX=1402 GN=bg1 PE=1 SV=1
sp|P53301|CRH1_YEAST	6.89e-07	183	334	104	250	Probable glycosidase CRH1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CRH1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.817031	0.182975

TMHMM  Annotations     

Start	End
28	50