CAZyme Information: ACLA_092460-t26_1-p1

Basic Information

• Species: Aspergillus clavatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus clavatus
• CAZyme ID: ACLA_092460-t26_1-p1
• CAZy Family: GT32
• CAZyme Description: UDP-glucose:glycoprotein glucosyltransferase, putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1492		166732.40	4.7561

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AclavatusNRRL1	9428	344612	287	9141

• Gene Location: location=DS027052:1485299-1489989(+)

Full Sequence      

MAIRSNIVASWRQCFVVVAALSCLARASPSVNVALQASFDSAPYLLELLETTAEENSTAY
FPLLDRIAEGTFDDTTTEKELYDRFLQVVHDDGHLSTPELLSSFKLSLAIRSAAPRISAH
YQFYNASVQHSLMAAQDAACPVWVHSEGKQYCSSSMERAQQDMSDEADSRELPFDRVLGD
TSLPPAILYADIASPMFKDFHHTLTGLVKEGQVSYRVRYRPPQHWSSRPLFVSGYGVELA
LKRTDYIVIDDRDAEQRSQNTIASTEPSDGEDSPDDLRPLSSSEVSRLGINTASYVMDSD
TPLDTLVKISQDFPKYSGKIAAYNTSTTLLQHIRASRLDLLPSGANVMWINGIQIDPRQI
DAFSLLDHLRRERRSIDKFRSTGLSAQEAVDLLCHESLAETLAQDAPSRYNYQDEIEGGG
VIIWMNDLEKDAKYQSWPSEVSAYLQPIYPGQLPAVRRDAHNIVVPVDLTNPEDMELIVK
TIQVFVKKKIPVRFGLVPLASSPESIAQLKVAHYLQETYGLASLIQYLEEASDIQLSAAK
NKIGSPDKACLQHATKDHDVRPNKQVLTLEEILKSDDFETLASRAAKYQSRLGIRGGGSH
LLVNGVFIVRDEKWPQEMSMRVGRDLQTIQQGVMDGTIDDEMWLPQLFLSQAFDRRNPLI
VPEDAKDIRIVDIAKLSESGKGLSEALVIASKAGNAIDSKHLIVVGDFDSAKGLQLLVTA
LEYQEKNGEVEVVLIHNPIPELETESGSALLYRSLKVNGRTDAAQVLADLKAADAPMSSE
NEAQEMAQFWEAQQLLAGELGFSPGANGIVINGRAVGPLLDDSTLSVEDLGQLLAYEQRT
RVGPVADAAKDLGFESRLSSPLALAKLTSLTALSTVSDVPEGIFETTSNVRTDLFKKWND
SRSVITVSNSDDPAITIVVSIDPTSETAQRWLPILKVLSELASVRVRLSLNPRDEIQELP
IKRFYRYVLDSEPSFSDDGSLARPTATFSGVPMEALLTLGMDVPSPWLVAPKESIYDLDN
IKLSTLKPGSNVDAIYALEHILIEGHSRDLTTKTAPQGVQLVLGTDDNPHFADTIIMANL
GYFQFKAQPGLWKINLKPGRSQTIFNLDSVGGQGYSPQPGDENNEVSLLSFQGKTLFPSL
SRKKGQEMEDVLETGAKPGSAMDYVSKGFNFAQGVLSSVGVGSKHGSEKQADINIFSVAS
GHLYERMLNIMMVSVMRNTKHSVKFWFIEQFLSPSFKSFLPHLAEQYGFSYEMVTYKWPH
WLRAQREKQREIWGYKILFLDVLFPLSLDKVIFVDADQIVRTDLYDLVSLDLEGAPYGFT
PMCDSREEMEGFRFWKQGYWKNFLRGLPYHISALYVVDLNRFRALAAGDRLRGQYQMLSA
DPNSLSNLDQDLPNHMQHHIPIKSLPQEWLWCETWCSDESLGVARTIDLCNNPQTKEPKL
DRARRQVPEWTEYDDEIATLAKKLAEEQQQREEEKRSDDREEQESDWIKDEL

Enzyme Prediction     

EC	2.4.1.-:29

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT24	1193	1440	6.2e-134	0.9959677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
408203	Glyco_transf_24	0.0	1193	1460	1	268	Glucosyltransferase 24. This is the catalytic domain found in UDP-glucose:glycoprotein glucosyltransferase (UGGT). This domain belongs to glucosyltransferase 24 family (GT24) A-type domain. The GT domain displays the expected glycosyltransferase type A (GT-A) fold.
133054	GT8_HUGT1_C_like	0.0	1193	1440	1	248	The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity.
408201	Thioredoxin_14	4.78e-98	410	660	1	248	Thioredoxin-like domain. This is the third out of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
408199	Thioredoxin_12	1.05e-62	41	228	1	185	Thioredoxin-like domain. This is one of four TRXL(thioredoxin-like) domains found in UDP-glucose:glycoprotein glucosyltransferase (UGGT).
399437	UDP-g_GGTase	2.08e-61	1044	1153	1	109	UDP-glucose:Glycoprotein Glucosyltransferase. UDP-g_GGTase is an important, central component of the QC system in the ER for checking that glycoproteins are folded correctly. This QC prevents incorrectly folded glycoproteins from leaving the ER.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
UDD64319.1|GT24	0.0	8	1492	8	1487
BAE65008.1|GT24|2.4.1.-	0.0	8	1492	8	1487
QRD91624.1|GT24	0.0	8	1492	8	1487
QMW47293.1|GT24	0.0	8	1492	8	1487
QMW35231.1|GT24	0.0	8	1492	8	1487

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MU1_A	0.0	27	1466	7	1453	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5MZO_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],5N2J_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TRF_A Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5NV4_A	0.0	27	1466	7	1453	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
5Y7O_A	0.0	30	1159	5	1130	Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii],5Y7O_B Crystal structure of folding sensor region of UGGT from Thermomyces dupontii [Thermomyces dupontii]
6TRT_A	0.0	27	1466	7	1453	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]
6TS2_A	0.0	27	1466	7	1219	Chain A, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_B Chain B, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_C Chain C, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495],6TS2_D Chain D, UDP-glucose-glycoprotein glucosyltransferase-like protein,UDP-glucose-glycoprotein glucosyltransferase-like protein [Thermochaetoides thermophila DSM 1495]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|Q09140|UGGG_SCHPO	9.46e-291	18	1460	9	1424	UDP-glucose:glycoprotein glucosyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpt1 PE=1 SV=2
sp|Q6P5E4|UGGG1_MOUSE	2.00e-243	28	1460	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Mus musculus OX=10090 GN=Uggt1 PE=1 SV=4
sp|Q9NYU2|UGGG1_HUMAN	4.64e-242	28	1460	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Homo sapiens OX=9606 GN=UGGT1 PE=1 SV=3
sp|Q9JLA3|UGGG1_RAT	3.18e-241	28	1460	44	1523	UDP-glucose:glycoprotein glucosyltransferase 1 OS=Rattus norvegicus OX=10116 GN=Uggt1 PE=1 SV=2
sp|Q09332|UGGG_DROME	1.29e-232	31	1464	30	1511	UDP-glucose:glycoprotein glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=Uggt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	CS Position
0.000234	0.999755	CS pos: 27-28. Pr: 0.9746

TMHMM  Annotations     

There is no transmembrane helices in ACLA_092460-t26_1-p1.