CAZyme Information: ACLA_057860-t26_1-p1

Basic Information

• Species: Aspergillus clavatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus clavatus
• CAZyme ID: ACLA_057860-t26_1-p1
• CAZy Family: GH31
• CAZyme Description: mannosyltransferase PMTI

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
781		88699.92	8.2446

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AclavatusNRRL1	9428	344612	287	9141

• Gene Location: location=DS026990:244937-247462(+)

Full Sequence      

MSQSPSSSLRKRGGKKETYPPVPTDDVSSSPLSSPKLPVASKSQSEWDYRLALVVLTVLA
FITRFYKISYPNEVVFDEVHFGKFASYYLQRTYFFDVHPPFGKLLFALMGWLVGYDGHFL
FDNIGDSYIDNKVPYVALRAMPATLGALTIPVVFLIMWESGYSLPACVLAAGLVLFDNAH
VGEDRLILLDASLVLTMALSILCYVRFYKLRHEPFGRKWWKWLLLTGVCLSCVISTKYVG
VFTFVTIGAAVLVDLWNLLDINRPSGSLSMVHWGKHFAARAFALIIVPIFFYLFWFQVHF
AILTRSGPGDDFMTPEFQETLSDNAMAAQSVGIEYFDTITIRHKDTKVLLHSHWEKYPLR
YDDGRISSQGQQVTGYPFNDTNNNWQILPAVPLPGVDDKGHSVKNGDIIQLRHVGTDTVL
LTHDVASPYYPTNQEFTTVSHELANGERHNDTLFEIKIENGKAQQEFRTLSSHFKLIHVP
TRVAMWTHTNPLPEWAFKQAEINGNKNVLQSSNLWFVESIESLDESNPRASKEVRQVKHL
PFWRKYLELQRAMFFHNNALTSSHPYASEPFQWPFLLRGVSFWTKNDTREQIYFLGNPIG
WWIASSLLAVFAGVIGADQLSLRRGVDALEEIWGRGSRSRLYNSTAFLFLCWAAHYFPFW
LMGRQRFLHHYLPAHVASAMVTGALIEFIFNLQPISVPRPVKEDDPTGKSKAEASPGHFV
TAKERMGRQSLIAAWVATLVILSVTIWGFCFYAPLTYGTPGLDVAGVNSRKWLGYDLHFA
K

Enzyme Prediction     

EC	2.4.1.109:8

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	56	300	2.7e-74	0.9910313901345291

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
224839	PMT1	0.0	48	781	22	699	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
396786	PMT	8.04e-85	54	304	1	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
406576	PMT_4TMC	1.53e-76	544	773	1	197	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
397103	MIR	2.70e-17	345	498	7	164	MIR domain. The MIR (protein mannosyltransferase, IP3R and RyR) domain is a domain that may have a ligand transferase function.
197746	MIR	3.52e-06	333	390	4	57	Domain in ryanodine and inositol trisphosphate receptors and protein O-mannosyltransferases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCS03793.1|GT39	0.0	1	781	1	775
BCS15407.1|GT39	0.0	1	781	1	775
QMW29154.1|GT39	0.0	1	781	1	775
QMW41228.1|GT39	0.0	1	781	1	775
QRD85448.1|GT39	0.0	1	781	1	775

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6P25_A	1.13e-106	21	778	15	730	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_A Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
6P25_B	2.11e-98	34	674	49	681	Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_B Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]
6P28_A	7.69e-13	349	522	24	195	Crystal structure of the MIR domain (aa 337-532) of the S. cerevisiae mannosyltransferase Pmt2 [Saccharomyces cerevisiae]
6ZQP_A	1.08e-12	349	522	41	212	Structure of the Pmt2-MIR domain with bound ligands [Saccharomyces cerevisiae]
1T9F_A	3.06e-07	350	481	24	139	Structural genomics of Caenorhabditis elegans: Structure of a protein with unknown function [Caenorhabditis elegans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|O42933|PMT4_SCHPO	9.32e-259	4	773	3	767	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ogm4 PE=3 SV=1
sp|Q59X23|PMT4_CANAL	6.35e-256	6	781	2	755	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PMT4 PE=2 SV=2
sp|P46971|PMT4_YEAST	7.36e-241	44	781	47	762	Dolichyl-phosphate-mannose--protein mannosyltransferase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PMT4 PE=1 SV=1
sp|Q8R2R1|POMT1_MOUSE	1.49e-118	48	772	36	736	Protein O-mannosyl-transferase 1 OS=Mus musculus OX=10090 GN=Pomt1 PE=1 SV=1
sp|Q99PR0|POMT1_RAT	3.01e-118	48	772	36	736	Protein O-mannosyl-transferase 1 OS=Rattus norvegicus OX=10116 GN=Pomt1 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
0.999940	0.000049

TMHMM  Annotations     

Start	End
49	66
93	115
136	158
186	208
228	250
277	296
593	615
641	663
731	753