CAZyme Information: ACLA_015020-t26_1-p1

Basic Information

• Species: Aspergillus clavatus
• Lineage: Ascomycota; Eurotiomycetes; ; Aspergillaceae; Aspergillus; Aspergillus clavatus
• CAZyme ID: ACLA_015020-t26_1-p1
• CAZy Family: AA7
• CAZyme Description: alpha-1,3-mannosyltransferase (Alg3), putative

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
472	DS027049|CGC4	53247.42	9.7388

Genome Property

Genome Version/Assembly ID	Genes	Strain NCBI Taxon ID	Non Protein Coding Genes	Protein Coding Genes
FungiDB-61_AclavatusNRRL1	9428	344612	287	9141

• Gene Location: location=DS027049:1860395-1861947(-)

Full Sequence      

MELNDFCIATIITALIILCETLSLLLHPGLELSRKRTNTIGSLGLKPHAWKPALWDLNTI
DNMELKHLLGDLCMNPRHTRWIAPLLILGDAVLCALIIWRVPYTEIDWTTYMQQVSLYLS
GERDYTLIKGSTGPLVYPAAHVYIYSILYYLTDEGRDILLAQILFAIPYLATLAVVMSCY
RQAGAPPFLLLPLVLSKRLHSIFVLRLFNDGFAALAMWIAIFLFQKKKWTAGVVVWSTGV
AIKMTLLLLAPAIAVVLVLSLSLQPSIQLGALAVLIQILLGIPFLSHNTAGYIARSFELT
RQFMFKWTVNWRFVGEELFLSRKFSLALLALHIALLGLFATRVWLKPSGSKLPSFVQQLL
QRRYRTVSLSRTFIMRTMLSSLAIGLLCARSLHYQFFAYLAWATPFLLWQTGFHPIVVYA
VCMAQEWAWNIYPSTNGSSLVVILSLAVQVIGILWNANGRQIPENSPKEHVQ

Enzyme Prediction     

EC	2.4.1.258:10

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT58	83	439	8.2e-147	0.9972527472527473

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
398745	ALG3	0.0	84	439	1	358	ALG3 protein. The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAE62911.1|GT58	3.76e-208	63	472	1	413
UDD58266.1|GT58	3.76e-208	63	472	1	413
BCR98022.1|GT58	2.16e-207	63	472	1	413
BCS10476.1|GT58	2.16e-207	63	472	1	413
QMW45188.1|GT58	9.48e-207	58	472	136	553

PDB Hits     

ACLA_015020-t26_1-p1 has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
sp|A1CBE6|ALG3_ASPCL	0.0	1	472	1	472	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=alg3 PE=3 SV=1
sp|Q4WVG2|ALG3_ASPFU	7.04e-229	57	472	3	419	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=alg3 PE=3 SV=1
sp|A1DDZ3|ALG3_NEOFI	4.07e-226	63	472	1	411	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=alg3 PE=3 SV=1
sp|Q2U6A4|ALG3_ASPOR	6.68e-209	63	472	1	413	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=alg3 PE=3 SV=1
sp|A2RA94|ALG3_ASPNC	1.81e-181	63	472	1	409	Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=alg3 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	CS Position
1.000069	0.000000

TMHMM  Annotations     

Start	End
6	28
81	103
134	151
158	180
202	224
233	255
265	287
326	345
372	389
396	418
433	455