CAZyme3D

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Entry ID

Information for CAZyme ID: WIA20768.1

Basic Information

GenBank IDWIA20768.1
FamilyGT31
Sequence Length163
UniProt IDA0A383VH54(98.6,89.0)Download
Average pLDDT?62.46
CAZy50 ID183372
CAZy50 RepYes, WIA20768.1
Structure ClusterSC_GT31_clus139
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID3088
KingdomEukaryota
PhylumChlorophyta
ClassChlorophyceae
OrderSphaeropleales
FamilyScenedesmaceae
GenusTetradesmus
SpeciesTetradesmus obliquus

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MCGLSRQTGF  TAPGAEPRYN  YAARRAALRA  TWFPGHPEER  AWFESEHSVV  LRFIIGSSAN60
ASDELALQQE  EQEHGDFMRL  PLQEDYRALP  NKTRTFFRAV  TQQWDPDWVI  KMDDDVYLNT120
ARLLDAARQW  DVMGAQYVGC  MKHGVVWRQP  GALPVEAVSG  TNC163

Predicted 3D structure by AlphaFold2 with pLDDT = 62.46 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MCGLSRQTGF  TAPGAEPRYN  YAARRAALRA  TWFPGHPEER  AWFESEHSVV  LRFIIGSSAN60
ASDELALQQE  EQEHGDFMRL  PLQEDYRALP  NKTRTFFRAV  TQQWDPDWVI  KMDDDVYLNT120
ARLLDAARQW  DVMGAQYVGC  MKHGVVWRQP  GALPVEAVSG  TNC163

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GT31(24-146)

MRQGPHDAST  RAADSTILGL  TSGTAGIASA  PSPQLQQASL  TSRPQPAAAP  SQRVRQSASE60
QQQQQQQHQQ  QLLLQQPQQQ  LQQEGKAAGA  SILAGSYKHA  DGSLKGAAAV  APATHVDTAA120
SLSHSTTNSN  SQAVAASRTH  VRPQQAGGAT  LLRTTHSITA  AGGGLGGSKA  SILQLATAYE180
ESLAGLSMAL  QMTQQKAAET  AALRNMLGQA  LQRRDSMQQS  RDSTTDTSGR  RSNNAASKER240
HADGSSSVRA  ALEQDAVAEN  TGQGITQQQH  INITALAAGA  YATDATAAAA  AAAGSSHGSE300
NAAGISGGAT  PVTSTGGAAA  GVACGRGNGP  RIRLFIGVYT  GFTAPGTEPR  YNYAARRAAL360
RATWFPGHPE  ERARFESEHS  VVLRFIIGSS  ANASDELALQ  QEEQEHGDFM  RLPLQEDYRA420
LPNKTRTFFR  AVTQQWDPDW  VIKMDDDVYL  NTARLLDAAR  QWDVMGAQYV  GCMKHGVVWR480
QPGARWYEPA  HLLVGPTYWL  HAYGSIYAIA  GPTARCVIGA  GFDRLRLLGN  EDTSVGLWML540
AADAAHFEDM  RLCSPMCT558

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help