CAZyme3D

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Entry ID

Information for CAZyme ID: WFN95587.1

Basic Information

GenBank IDWFN95587.1
FamilyGH23
Sequence Length220
UniProt IDA0A1P8YZN9(100,99.5)Download
Average pLDDT?76.35
CAZy50 ID177612
CAZy50 RepYes, WFN95587.1
Structure ClusterSC_GH23_clus144
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID67780
KingdomBacteria
PhylumPseudomonadota
ClassGammaproteobacteria
OrderEnterobacterales
FamilyHafniaceae
GenusEdwardsiella
SpeciesEdwardsiella ictaluri

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MMSKWVLLGL  ALLAWQSAGA  PLKWYRVTGE  DRVASEAPPA  IPRGDDYASL  IGQVAGKHAL60
DPQLIQAVVA  AESSYRYDSV  SEKGAMGLMQ  VMPETAARYG  HFALLEPRDN  LEAGTSYLST120
LLRRFNGRLD  LALAGYNAGE  GAVLRYGGTI  PPYPETQRYV  QRVLENYRQL  RSVVPASPAP180
PPQAEVEVHT  SGGVGDLGQL  WHLLTSGSPG  ETGSSASSGT  220

Predicted 3D structure by AlphaFold2 with pLDDT = 76.35 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MMSKWVLLGL  ALLAWQSAGA  PLKWYRVTGE  DRVASEAPPA  IPRGDDYASL  IGQVAGKHAL60
DPQLIQAVVA  AESSYRYDSV  SEKGAMGLMQ  VMPETAARYG  HFALLEPRDN  LEAGTSYLST120
LLRRFNGRLD  LALAGYNAGE  GAVLRYGGTI  PPYPETQRYV  QRVLENYRQL  RSVVPASPAP180
PPQAEVEVHT  SGGVGDLGQL  WHLLTSGSPG  ETGSSASSGT  220

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH23(58-171)

MSKWVLLGLA  LLAWQSAGAP  LKWYRVTGED  RVASEAPPAI  PRGDDYASLI  GQVAGKHALD60
PQLIQAVVAA  ESSYRYDSVS  EKGAMGLMQV  MPETAARYGH  FALLEPRDNL  EAGTSYLSTL120
LRRFNGRLDL  ALAGYNAGEG  AVLRYGGTIP  PYPETQRYVQ  RVLENYRQLR  SVVPASPAPP180
PQAEVEVHTS  GGVGDLGQLW  HLLTSGSPGE  TGSSASSGT219

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help