CAZyme3D

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Entry ID

Information for CAZyme ID: QPA53933.1

Basic Information

GenBank IDQPA53933.1
FamilyCE4
Sequence Length274
UniProt IDB1HUQ5(100,100)Download
Average pLDDT?88.63
CAZy50 ID164861
CAZy50 RepYes, QPA53933.1
Structure ClusterSC_CE4_clus45
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID1421
KingdomBacteria
PhylumBacillota
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusLysinibacillus
SpeciesLysinibacillus sphaericus

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MWKQHIVGAV  IATFIIAAGI  SINPYFAAGS  DEHHWGFKRA  KNGEQAEAGA  QLDQLLDKYG60
AIYKGKADKK  IAYLTFDNGY  ENGFTESILD  TLKKEDVPAT  FFLTGHYLDS  ASDLVKRMVK120
DGHTIGNHSY  GHPNMARLTP  EGMRAEWRKF  DGKLREMTGI  DRTTYARPPE  GTFNAKLLEV180
GNAEGYRHIF  WSVAFKDWMK  DERRGADYAY  NALMEQLHPG  AVILMHTVAQ  DNAEALPMFI240
AEAKKQGYTF  LSLDDLVLEY  EDFPVALQSS  APSL274

Predicted 3D structure by AlphaFold2 with pLDDT = 88.63 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MWKQHIVGAV  IATFIIAAGI  SINPYFAAGS  DEHHWGFKRA  KNGEQAEAGA  QLDQLLDKYG60
AIYKGKADKK  IAYLTFDNGY  ENGFTESILD  TLKKEDVPAT  FFLTGHYLDS  ASDLVKRMVK120
DGHTIGNHSY  GHPNMARLTP  EGMRAEWRKF  DGKLREMTGI  DRTTYARPPE  GTFNAKLLEV180
GNAEGYRHIF  WSVAFKDWMK  DERRGADYAY  NALMEQLHPG  AVILMHTVAQ  DNAEALPMFI240
AEAKKQGYTF  LSLDDLVLEY  EDFPVALQSS  APSL274

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : CE4(63-188)

MWKQHIVGAV  IATFIIAAGI  SINPYFAAGS  DEHHWGFKRA  KNGEQAEAGA  QLDQLLDKYG60
AIYKGKADKK  IAYLTFDNGY  ENGFTESILD  TLKKEDVPAT  FFLTGHYLDS  ASDLVKRMVK120
DGHTIGNHSY  GHPNMARLTP  EGMRAEWRKF  DGKLREMTGI  DRTTYARPPE  GTFNAKLLEV180
GNAEGYRHIF  WSVAFKDWMK  DERRGADYAY  NALMEQLHPG  AVILMHTVAQ  DNAEALPMFI240
AEAKKQGYTF  LSLDDLVLEY  EDFPVALQSS  APSL274

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help