CAZyme3D

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Entry ID

Information for CAZyme ID: QNJ46226.1

Basic Information

GenBank IDQNJ46226.1
FamilyGH25
Sequence Length232
UniProt IDA0A7S6G7W7(100,100)Download
Average pLDDT?92.59
CAZy50 ID175831
CAZy50 RepYes, QNJ46226.1
Structure ClusterSC_GH25_clus96
EC Number(s)3.2.1.17
Substrates(s)peptidoglycan

Taxonomy

Tax ID101121
KingdomEukaryota
PhylumMucoromycota
ClassMucoromycetes
OrderMucorales
FamilyLichtheimiaceae
GenusThermomucor
SpeciesThermomucor indicae-seudaticae

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MKFSIFAIAA  AAAAAVATLV  QGYQTGLDVS  ALTSTSSFSC  AKNLGYDHVI  ARCYMEAYGN60
NPGGKVDPNC  YSNYKNAKAA  GFTSVDIYMF  PCTGRSTCKS  PATQVQEIVD  YVGAHKMIVG120
TLWLDVEVDS  AANNWPSTSE  ARSTLRAFKT  ALDKSGWKWG  VYSSKSQWTR  ITGSASWVLD180
PSVPLWYSHY  DDTLSFSDYP  SHAFGGWSKP  TIKQYTGDAS  FCSASWDKNY  YG232

Predicted 3D structure by AlphaFold2 with pLDDT = 92.59 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MKFSIFAIAA  AAAAAVATLV  QGYQTGLDVS  ALTSTSSFSC  AKNLGYDHVI  ARCYMEAYGN60
NPGGKVDPNC  YSNYKNAKAA  GFTSVDIYMF  PCTGRSTCKS  PATQVQEIVD  YVGAHKMIVG120
TLWLDVEVDS  AANNWPSTSE  ARSTLRAFKT  ALDKSGWKWG  VYSSKSQWTR  ITGSASWVLD180
PSVPLWYSHY  DDTLSFSDYP  SHAFGGWSKP  TIKQYTGDAS  FCSASWDKNY  YG232

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH25(28-219)

MKFSIFAIAA  AAAAAVATLV  QGYQTGLDVS  ALTSTSSFSC  AKNLGYDHVI  ARCYMEAYGN60
NPGGKVDPNC  YSNYKNAKAA  GFTSVDIYMF  PCTGRSTCKS  PATQVQEIVD  YVGAHKMIVG120
TLWLDVEVDS  AANNWPSTSE  ARSTLRAFKT  ALDKSGWKWG  VYSSKSQWTR  ITGSASWVLD180
PSVPLWYSHY  DDTLSFSDYP  SHAFGGWSKP  TIKQYTGDAS  FCSASWDKNY  YG232

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help