CAZyme3D

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Entry ID

Information for CAZyme ID: CAK20037.1

Basic Information

GenBank IDCAK20037.1
FamilyCE4
Sequence Length274
UniProt IDA0AGA5(100,100)Download
Average pLDDT?86.87
CAZy50 ID165030
CAZy50 RepYes, CAK20037.1
Structure ClusterSC_CE4_clus45
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID386043
KingdomBacteria
PhylumBacillota
ClassBacilli
OrderBacillales
FamilyListeriaceae
GenusListeria
SpeciesListeria welshimeri

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MPMELKTEKD  GGAEMKKIYI  IMTSILLCII  CVVVGISIFL  KQDEVVQQPK  EKISKKQVLS60
DKEQPSSSNE  GKKVAYLTID  DGPTEYMSKI  LTALKKENVK  ATFFLVGNRI  TGDRKKDIKE120
AAEEGHSIGL  HSMSHVAKRL  YKDKQFIPEI  EKESKLLNTI  LDKKTKLVRA  PYGSTYLNDD180
QVGQLKKDKY  RLLDWNIDSE  DWKHKGKPEK  MVSFIKEELN  KFKKISPVIL  IHETEDLLKA240
IPDLVKTIRA  KGFELKPYFE  DNNFHLNFKK  DPQL274

Predicted 3D structure by AlphaFold2 with pLDDT = 86.87 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MPMELKTEKD  GGAEMKKIYI  IMTSILLCII  CVVVGISIFL  KQDEVVQQPK  EKISKKQVLS60
DKEQPSSSNE  GKKVAYLTID  DGPTEYMSKI  LTALKKENVK  ATFFLVGNRI  TGDRKKDIKE120
AAEEGHSIGL  HSMSHVAKRL  YKDKQFIPEI  EKESKLLNTI  LDKKTKLVRA  PYGSTYLNDD180
QVGQLKKDKY  RLLDWNIDSE  DWKHKGKPEK  MVSFIKEELN  KFKKISPVIL  IHETEDLLKA240
IPDLVKTIRA  KGFELKPYFE  DNNFHLNFKK  DPQL274

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : CE4(68-192)

MPMELKTEKD  GGAEMKKIYI  IMTSILLCII  CVVVGISIFL  KQDEVVQQPK  EKISKKQVLS60
DKEQPSSSNE  GKKVAYLTID  DGPTEYMSKI  LTALKKENVK  ATFFLVGNRI  TGDRKKDIKE120
AAEEGHSIGL  HSMSHVAKRL  YKDKQFIPEI  EKESKLLNTI  LDKKTKLVRA  PYGSTYLNDD180
QVGQLKKDKY  RLLDWNIDSE  DWKHKGKPEK  MVSFIKEELN  KFKKISPVIL  IHETEDLLKA240
IPDLVKTIRA  KGFELKPYFE  DNNFHLNFKK  DPQL274

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help