CAZyme3D

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Entry ID

Information for CAZyme ID: BDR59839.1

Basic Information

GenBank IDBDR59839.1
FamilyGH32
Sequence Length474
UniProt IDBDR59839.1(MOD)Download
Average pLDDT?95.14
CAZy50 ID74705
CAZy50 RepYes, BDR59839.1
Structure ClusterSC_GH32_clus114
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID2976676
KingdomBacteria
PhylumBacillota
ClassBacilli
OrderLactobacillales
FamilyLactobacillaceae
GenusLactobacillus
SpeciesLactobacillus xylocopicola

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MEKKATNNHQ  IYYQPKNVWV  GDIMPYANDG  KFYIYHQRDQ  RVHGPITDPF  GWALATTKDF60
VNYQDYGESL  KRGSDQDWDQ  FVYAGSVFEA  NGTIHAFYTG  YNKEFLQAGK  TSQILLHATS120
NDFIHWKKSI  NALKLTPQPG  YDIRNWRDPS  VIWDERKQEY  LLILGARKGK  DKHKLTGRLV180
KYTSKDLENW  QFEGDFWAPN  LYTMFEMPQL  FQMGDWWYLV  YSEYSVKNKV  FYRMSKSLDG240
PWLKPKDEAF  DGRAYYAART  AFDGKRRVLF  GWVPTKLNED  DMSNYEWGGT  FVPQEVYQRR300
DKTLGVRPIA  EICEAFTSKQ  KIPAQKIGAV  DTLQEKTLVE  NTGYHFYFHT  TISFSESTTD360
FSIRLYKNNA  TDESYEFRFN  LDENQLTLDK  NPCYRWYQMM  DKGLNRPINL  EANQDYDLKV420
IVDDNILSLY  LDGVALNTRV  YHKFGTSLAL  TATNGNLSLS  NIEFSNKYQN  AKKG474

Predicted 3D structure by AlphaFold2 with pLDDT = 95.14 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MEKKATNNHQ  IYYQPKNVWV  GDIMPYANDG  KFYIYHQRDQ  RVHGPITDPF  GWALATTKDF60
VNYQDYGESL  KRGSDQDWDQ  FVYAGSVFEA  NGTIHAFYTG  YNKEFLQAGK  TSQILLHATS120
NDFIHWKKSI  NALKLTPQPG  YDIRNWRDPS  VIWDERKQEY  LLILGARKGK  DKHKLTGRLV180
KYTSKDLENW  QFEGDFWAPN  LYTMFEMPQL  FQMGDWWYLV  YSEYSVKNKV  FYRMSKSLDG240
PWLKPKDEAF  DGRAYYAART  AFDGKRRVLF  GWVPTKLNED  DMSNYEWGGT  FVPQEVYQRR300
DKTLGVRPIA  EICEAFTSKQ  KIPAQKIGAV  DTLQEKTLVE  NTGYHFYFHT  TISFSESTTD360
FSIRLYKNNA  TDESYEFRFN  LDENQLTLDK  NPCYRWYQMM  DKGLNRPINL  EANQDYDLKV420
IVDDNILSLY  LDGVALNTRV  YHKFGTSLAL  TATNGNLSLS  NIEFSNKYQN  AKKG474

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH32(13-301)+CBM66(338-462)

MEKKATNNHQ  IYYQPKNVWV  GDIMPYANDG  KFYIYHQRDQ  RVHGPITDPF  GWALATTKDF60
VNYQDYGESL  KRGSDQDWDQ  FVYAGSVFEA  NGTIHAFYTG  YNKEFLQAGK  TSQILLHATS120
NDFIHWKKSI  NALKLTPQPG  YDIRNWRDPS  VIWDERKQEY  LLILGARKGK  DKHKLTGRLV180
KYTSKDLENW  QFEGDFWAPN  LYTMFEMPQL  FQMGDWWYLV  YSEYSVKNKV  FYRMSKSLDG240
PWLKPKDEAF  DGRAYYAART  AFDGKRRVLF  GWVPTKLNED  DMSNYEWGGT  FVPQEVYQRR300
DKTLGVRPIA  EICEAFTSKQ  KIPAQKIGAV  DTLQEKTLVE  NTGYHFYFHT  TISFSESTTD360
FSIRLYKNNA  TDESYEFRFN  LDENQLTLDK  NPCYRWYQMM  DKGLNRPINL  EANQDYDLKV420
IVDDNILSLY  LDGVALNTRV  YHKFGTSLAL  TATNGNLSLS  NIEFSNKYQN  AKKG474

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help