CAZyme3D

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Entry ID

Information for CAZyme ID: BCQ32371.1

Basic Information

GenBank IDBCQ32371.1
FamilyGH32
Sequence Length297
UniProt IDBCQ32371.1(MOD)Download
Average pLDDT?95.66
CAZy50 ID157364
CAZy50 RepYes, BCQ32371.1
Structure ClusterSC_GH32_clus4
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID1613
KingdomBacteria
PhylumBacillota
ClassBacilli
OrderLactobacillales
FamilyLactobacillaceae
GenusLimosilactobacillus
SpeciesLimosilactobacillus fermentum

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MLGSQNTAGF  GRLLLYQSTD  LLHWQEAGII  DQAHDLATEG  FMWECPDLFS  LGEQDVMVTS60
PMGIKATTHE  NLNSSQVDYS  IGHLDYGTPH  FSGTKLTELD  YGHNFYATQT  MLTPDQRRIQ120
IGWMSPFEEQ  LQEHTDGWAG  CLTFPRELTI  QNNRLIQQPI  WELVNLRQSQ  DVNETTLVNA180
ETPLTLSDPQ  HCEIDLHWPK  ELLEEFSWQL  NDQLGKLLAL  TYADGQLTLY  RRGADAYRYA240
SAAQLRSLQI  LVDTSSVEVF  VNDGESTFTE  RYYATGPVAH  FLNGTGCTTE  VVAYRLA297

Predicted 3D structure by AlphaFold2 with pLDDT = 95.66 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MLGSQNTAGF  GRLLLYQSTD  LLHWQEAGII  DQAHDLATEG  FMWECPDLFS  LGEQDVMVTS60
PMGIKATTHE  NLNSSQVDYS  IGHLDYGTPH  FSGTKLTELD  YGHNFYATQT  MLTPDQRRIQ120
IGWMSPFEEQ  LQEHTDGWAG  CLTFPRELTI  QNNRLIQQPI  WELVNLRQSQ  DVNETTLVNA180
ETPLTLSDPQ  HCEIDLHWPK  ELLEEFSWQL  NDQLGKLLAL  TYADGQLTLY  RRGADAYRYA240
SAAQLRSLQI  LVDTSSVEVF  VNDGESTFTE  RYYATGPVAH  FLNGTGCTTE  VVAYRLA297

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH32(2-159)

MLGSQNTAGF  GRLLLYQSTD  LLHWQEAGII  DQAHDLATEG  FMWECPDLFS  LGEQDVMVTS60
PMGIKATTHE  NLNSSQVDYS  IGHLDYGTPH  FSGTKLTELD  YGHNFYATQT  MLTPDQRRIQ120
IGWMSPFEEQ  LQEHTDGWAG  CLTFPRELTI  QNNRLIQQPI  WELVNLRQSQ  DVNETTLVNA180
ETPLTLSDPQ  HCEIDLHWPK  ELLEEFSWQL  NDQLGKLLAL  TYADGQLTLY  RRGADAYRYA240
SAAQLRSLQI  LVDTSSVEVF  VNDGESTFTE  RYYATGPVAH  FLNGTGCTTE  VVAYRLA297

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help