CAZyme3D

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Entry ID

Information for CAZyme ID: BCJ62042.1

Basic Information

GenBank IDBCJ62042.1
FamilyCBM35
Sequence Length266
UniProt IDA0A2W2BVA7(100,100)Download
Average pLDDT?72.19
CAZy50 ID167377
CAZy50 RepYes, BCJ62042.1
Structure ClusterSC_CBM35_clus25
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID515350
KingdomBacteria
PhylumActinomycetota
ClassActinomycetes
OrderMicromonosporales
FamilyMicromonosporaceae
GenusMicromonospora
SpeciesMicromonospora endophytica

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MPNDALSDAA  DPVPRSPGGV  PDPDRSARAR  HWVGISSLAG  AIALAAIAVP  PLLVPDERRP60
DGVGSAAPDG  TVDPTTTATL  PGGAPTTAPG  AAGSGPTTGP  TPPSRAPDST  PQPAGSLPGP120
APNRPTRTAA  PPPSATTGDA  TPPASFRPVS  VEAEDPGNLL  SEGAGIVDCA  ACAGGARVRY180
LGRLTAYLTT  ATAGERTITV  SYSTKGDRQL  KISINGAAPT  THHLTGTAWD  TPRTFQYAAT240
VPAGRVSFIF  YNDTGPPPDI  DKISIS266

Predicted 3D structure by AlphaFold2 with pLDDT = 72.19 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MPNDALSDAA  DPVPRSPGGV  PDPDRSARAR  HWVGISSLAG  AIALAAIAVP  PLLVPDERRP60
DGVGSAAPDG  TVDPTTTATL  PGGAPTTAPG  AAGSGPTTGP  TPPSRAPDST  PQPAGSLPGP120
APNRPTRTAA  PPPSATTGDA  TPPASFRPVS  VEAEDPGNLL  SEGAGIVDCA  ACAGGARVRY180
LGRLTAYLTT  ATAGERTITV  SYSTKGDRQL  KISINGAAPT  THHLTGTAWD  TPRTFQYAAT240
VPAGRVSFIF  YNDTGPPPDI  DKISIS266

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : CBM35(163-264)

MPNDALSDAA  DPVPRSPGGV  PDPDRSARAR  HWVGISSLAG  AIALAAIAVP  PLLVPDERRP60
DGVGSAAPDG  TVDPTTTATL  PGGAPTTAPG  AAGSGPTTGP  TPPSRAPDST  PQPAGSLPGP120
APNRPTRTAA  PPPSATTGDA  TPPASFRPVS  VEAEDPGNLL  SEGAGIVDCA  ACAGGARVRY180
LGRLTAYLTT  ATAGERTITV  SYSTKGDRQL  KISINGAAPT  THHLTGTAWD  TPRTFQYAAT240
VPAGRVSFIF  YNDTGPPPDI  DKISIS266

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help