CAZyme3D

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Entry ID

Information for CAZyme ID: BBE50261.1

Basic Information

GenBank IDBBE50261.1
FamilyGH20
Sequence Length340
UniProt IDA0A2Z6G9P7(100,100)Download
Average pLDDT?86.45
CAZy50 ID137017
CAZy50 RepYes, BBE50261.1
Structure ClusterSC_GH20_clus34
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID1188319
KingdomBacteria
PhylumPseudomonadota
ClassBetaproteobacteria
OrderNitrosomonadales
FamilyGallionellaceae
GenusFerriphaselus
SpeciesFerriphaselus amnicola

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MFSRTAFSFC  VFLMLLVGAR  YAVAAEVSLH  NSGAAPSFKL  RVLHIPLQYL  QPSEVKRLIK60
KAADAKFNAI  ILAINGMVAL  NSLPAVIDKA  WSKDELRDVA  KYTNALGIKV  IPEVKLLSHQ120
KLFFAEHFPD  LMFDHETYDP  NNPAVYKKVL  PILDELVELL  HPSAIHIGHD  EVNGAYQSKT180
LLGRWKLMLP  AVLFTKDVLV  IYTHLRSLGV  ETWMWGDMLL  GADEFPDMER  SPLNGGAGGY240
GKKLRIRLPK  DIVICDWHYA  DKQADFPSLR  ALRSDGFRVL  GATYLDKNTM  HGFSQYASQN300
GGDGMIATLW  VYPMRKDWET  TYQIIRESGA  VFNRDFPDEK  340

Predicted 3D structure by AlphaFold2 with pLDDT = 86.45 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MFSRTAFSFC  VFLMLLVGAR  YAVAAEVSLH  NSGAAPSFKL  RVLHIPLQYL  QPSEVKRLIK60
KAADAKFNAI  ILAINGMVAL  NSLPAVIDKA  WSKDELRDVA  KYTNALGIKV  IPEVKLLSHQ120
KLFFAEHFPD  LMFDHETYDP  NNPAVYKKVL  PILDELVELL  HPSAIHIGHD  EVNGAYQSKT180
LLGRWKLMLP  AVLFTKDVLV  IYTHLRSLGV  ETWMWGDMLL  GADEFPDMER  SPLNGGAGGY240
GKKLRIRLPK  DIVICDWHYA  DKQADFPSLR  ALRSDGFRVL  GATYLDKNTM  HGFSQYASQN300
GGDGMIATLW  VYPMRKDWET  TYQIIRESGA  VFNRDFPDEK  340

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH20(48-287)

MFSRTAFSFC  VFLMLLVGAR  YAVAAEVSLH  NSGAAPSFKL  RVLHIPLQYL  QPSEVKRLIK60
KAADAKFNAI  ILAINGMVAL  NSLPAVIDKA  WSKDELRDVA  KYTNALGIKV  IPEVKLLSHQ120
KLFFAEHFPD  LMFDHETYDP  NNPAVYKKVL  PILDELVELL  HPSAIHIGHD  EVNGAYQSKT180
LLGRWKLMLP  AVLFTKDVLV  IYTHLRSLGV  ETWMWGDMLL  GADEFPDMER  SPLNGGAGGY240
GKKLRIRLPK  DIVICDWHYA  DKQADFPSLR  ALRSDGFRVL  GATYLDKNTM  HGFSQYASQN300
GGDGMIATLW  VYPMRKDWET  TYQIIRESGA  VFNRDFPDEK  340

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help