CAZyme3D

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Entry ID

Information for CAZyme ID: AXT38462.1

Basic Information

GenBank IDAXT38462.1
FamilyGH32
Sequence Length238
UniProt IDA0A3A9UPK6(100,99.6)Download
Average pLDDT?93.72
CAZy50 ID174651
CAZy50 RepYes, AXT38462.1
Structure ClusterSC_GH32_clus114
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID1714845
KingdomBacteria
PhylumPseudomonadota
ClassGammaproteobacteria
OrderAlteromonadales
FamilyAlteromonadaceae
GenusAlteromonas
SpeciesAlteromonas sp. BL110

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MGPNGGSATQ  YFIGSFDGIE  FVPQDDEIRW  LDHGTDCYAG  ITWDNTPNIL  EERVYTAWMS60
NWDYANNTPD  DTWRGSMTLP  RRIKLGKRSN  SYRLLTPAFP  NFSEYGTQEA  DFSRKQALPR120
AYKLSLNISP  SEEQTDIVWT  NKEGEVFSIS  IDLKRSEITV  DRSQTGFFEK  GFDSASTIPI180
VFDTSDTICL  EIYVDACSFE  CFFNDGEQCI  TSLVFPSSSL  NNVNISEHVT  KISLTDFT238

Predicted 3D structure by AlphaFold2 with pLDDT = 93.72 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MGPNGGSATQ  YFIGSFDGIE  FVPQDDEIRW  LDHGTDCYAG  ITWDNTPNIL  EERVYTAWMS60
NWDYANNTPD  DTWRGSMTLP  RRIKLGKRSN  SYRLLTPAFP  NFSEYGTQEA  DFSRKQALPR120
AYKLSLNISP  SEEQTDIVWT  NKEGEVFSIS  IDLKRSEITV  DRSQTGFFEK  GFDSASTIPI180
VFDTSDTICL  EIYVDACSFE  CFFNDGEQCI  TSLVFPSSSL  NNVNISEHVT  KISLTDFT238

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) :

MHSTLRETSP  SQNGKWEPHF  HFYKFGHWIN  DPNGLFYLDG  KYHLFFQLNP  DATVWGNMHW60
GAAESTDLIN  WVHKPIALYA  EPEGLGYIFS  GGAIVDEKNT  SGFGIDNVPP  IIATFTQQSV120
KEEQVQSIAY  SVDGGNTFSM  FNENPVIANP  GIKDFRDPKV  VWFDGQDGQY  WVKAVVAGHC180
VHFYKSENLK  TWTKLSEFGE  GVGAHGGVWE  CPDLFPLLCE  ETGQQFWVLL  ISINPGGPNG240
GSATQYFIGS  FDGIEFVPQD  DEIRWLDHGT  DCYAGITWDN  TPNILEERVY  TAWMSNWDYA300
NNTPDDTWRG  SMTLPRRIKL  GKRSNSYRLL  TPAFPNFSEY  GTQEADFSRK  QALPRAYKLS360
LNISPSEEQT  DIVWTNKEGE  VFSISIDLKR  SEITVDRSQT  GFFEKGFDSA  STIPIVFDTS420
DTICLEIYVD  ACSFECFFND  GEQCITSLVF  PSSSLNNVNI  SEHVTKISLT  DFT473

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help