CAZyme3D

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Entry ID

Information for CAZyme ID: AOF82556.1

Basic Information

GenBank IDAOF82556.1
FamilyGH23
Sequence Length215
UniProt IDA0A1B3LNZ4(100,100)Download
Average pLDDT?80.72
CAZy50 ID178176
CAZy50 RepYes, AOF82556.1
Structure ClusterSC_GH23_clus144
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID1842540
KingdomBacteria
PhylumPseudomonadota
ClassBetaproteobacteria
OrderNitrosomonadales
FamilySterolibacteriaceae
GenusMethyloversatilis
SpeciesMethyloversatilis sp. RAC08

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MMRRMFVLLL  AGLPWLSHAN  GSDYQLRLGD  GTTYRLLQPE  LLRVSEVEQR  EARRQALSGL60
PFAAQIEQAA  VAGGIDPELL  HAVVQAESAY  NPHAVSPKGA  LGLAQMMPAT  ARAYGVTDAL120
KPVDNLRASA  RHLRDLLDHF  GDVELALSAY  NAGAGAVRRF  GGMPPYAETR  AYVPRVSDRY180
EALKRLAAPP  EPVVPPSPYK  LRADSSEMRL  IQSAN215

Predicted 3D structure by AlphaFold2 with pLDDT = 80.72 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MMRRMFVLLL  AGLPWLSHAN  GSDYQLRLGD  GTTYRLLQPE  LLRVSEVEQR  EARRQALSGL60
PFAAQIEQAA  VAGGIDPELL  HAVVQAESAY  NPHAVSPKGA  LGLAQMMPAT  ARAYGVTDAL120
KPVDNLRASA  RHLRDLLDHF  GDVELALSAY  NAGAGAVRRF  GGMPPYAETR  AYVPRVSDRY180
EALKRLAAPP  EPVVPPSPYK  LRADSSEMRL  IQSAN215

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH23(76-183)

MMRRMFVLLL  AGLPWLSHAN  GSDYQLRLGD  GTTYRLLQPE  LLRVSEVEQR  EARRQALSGL60
PFAAQIEQAA  VAGGIDPELL  HAVVQAESAY  NPHAVSPKGA  LGLAQMMPAT  ARAYGVTDAL120
KPVDNLRASA  RHLRDLLDHF  GDVELALSAY  NAGAGAVRRF  GGMPPYAETR  AYVPRVSDRY180
EALKRLAAPP  EPVVPPSPYK  LRADSSEMRL  IQSAN215

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help