CAZyme3D

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Entry ID

Information for CAZyme ID: AGF55638.1

Basic Information

GenBank IDAGF55638.1
FamilyGH5_1
Sequence Length493
UniProt IDM1MH26(100,100)Download
Average pLDDT?91.89
CAZy50 ID70359
CAZy50 RepYes, AGF55638.1
Structure ClusterSC_GH5_clus237
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID931276
KingdomBacteria
PhylumBacillota
ClassClostridia
OrderEubacteriales
FamilyClostridiaceae
GenusClostridium
SpeciesClostridium saccharoperbutylacetonicum

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MKIKVNYIAI  IMTMLFSLMA  PINVLAAEND  NSDDYLHTDG  SKIFDAEGNE  VRLTGIAWFG60
NETPNYSYHG  LWANTLDDIL  DKVADNGFNL  LRVPLSVQLV  NEWRQGNYPM  PDSINDYVNP120
DLKNKNSLEI  LDKSVSVCKK  KGIKVMLDMH  RVIDGSQSNA  WYTDKYSVED  YEECWKWLTN180
HYKNDDTVIA  MDIFNEPHGK  AYRQEQCAKW  DESTDADNWK  HEAEKVGKEI  LDINPNLLIM240
VEGVETYPKE  GKDYSSKDVN  NYYGTWWGGN  LRGVQDHPIK  IENHPNQVVY  SPHDYGPGVS300
NQPWFENGFT  KESLVKEVWE  PNWFYIQQKN  IAPLLIGEWG  GNMDGSDNQK  WMQGLADFIK360
ENNINHTFWC  LNPNSGDTGG  ILGYDFKTVD  TAKMALVKPT  LWQEKESGKF  IGLDHKINLG420
KNGTHVAANT  NVNPAVKLGD  VNGDGSVDTM  DYIALQKYVM  DSSNTINTKN  ADINGDNRIN480
TADLLSLKKL  ILQ493

Predicted 3D structure by AlphaFold2 with pLDDT = 91.89 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MKIKVNYIAI  IMTMLFSLMA  PINVLAAEND  NSDDYLHTDG  SKIFDAEGNE  VRLTGIAWFG60
NETPNYSYHG  LWANTLDDIL  DKVADNGFNL  LRVPLSVQLV  NEWRQGNYPM  PDSINDYVNP120
DLKNKNSLEI  LDKSVSVCKK  KGIKVMLDMH  RVIDGSQSNA  WYTDKYSVED  YEECWKWLTN180
HYKNDDTVIA  MDIFNEPHGK  AYRQEQCAKW  DESTDADNWK  HEAEKVGKEI  LDINPNLLIM240
VEGVETYPKE  GKDYSSKDVN  NYYGTWWGGN  LRGVQDHPIK  IENHPNQVVY  SPHDYGPGVS300
NQPWFENGFT  KESLVKEVWE  PNWFYIQQKN  IAPLLIGEWG  GNMDGSDNQK  WMQGLADFIK360
ENNINHTFWC  LNPNSGDTGG  ILGYDFKTVD  TAKMALVKPT  LWQEKESGKF  IGLDHKINLG420
KNGTHVAANT  NVNPAVKLGD  VNGDGSVDTM  DYIALQKYVM  DSSNTINTKN  ADINGDNRIN480
TADLLSLKKL  ILQ493

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH5_1(45-380)

MKIKVNYIAI  IMTMLFSLMA  PINVLAAEND  NSDDYLHTDG  SKIFDAEGNE  VRLTGIAWFG60
NETPNYSYHG  LWANTLDDIL  DKVADNGFNL  LRVPLSVQLV  NEWRQGNYPM  PDSINDYVNP120
DLKNKNSLEI  LDKSVSVCKK  KGIKVMLDMH  RVIDGSQSNA  WYTDKYSVED  YEECWKWLTN180
HYKNDDTVIA  MDIFNEPHGK  AYRQEQCAKW  DESTDADNWK  HEAEKVGKEI  LDINPNLLIM240
VEGVETYPKE  GKDYSSKDVN  NYYGTWWGGN  LRGVQDHPIK  IENHPNQVVY  SPHDYGPGVS300
NQPWFENGFT  KESLVKEVWE  PNWFYIQQKN  IAPLLIGEWG  GNMDGSDNQK  WMQGLADFIK360
ENNINHTFWC  LNPNSGDTGG  ILGYDFKTVD  TAKMALVKPT  LWQEKESGKF  IGLDHKINLG420
KNGTHVAANT  NVNPAVKLGD  VNGDGSVDTM  DYIALQKYVM  DSSNTINTKN  ADINGDNRIN480
TADLLSLKKL  ILQ493

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help