CAZyme3D

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Entry ID

Information for CAZyme ID: AFS48010.1

Basic Information

GenBank IDAFS48010.1
FamilyGT17
Sequence Length271
UniProt IDJ9YYH6(100,100)Download
Average pLDDT?93.26
CAZy50 ID165990
CAZy50 RepYes, AFS48010.1
Structure ClusterSC_GT17_clus32
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID859653
KingdomBacteria
PhylumPseudomonadota
ClassAlphaproteobacteria
OrderCandidatus Pelagibacterales
FamilyCandidatus Pelagibacteraceae
Genus
Speciesalpha proteobacterium HIMB5

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MTKLYDCFSY  WDEDLLLDLR  LNILNDYVDY  FVIVEGNKTW  QNNPKKLRFD  INKFSKFKDK60
IIYIPVEDMP  DGDNPYLREN  FQRNCITRGL  DKSKNEDIIL  ISDLDEIPNP  KKISIFKKKM120
KYAVFKQNHF  YYKFNFISKT  NPFWFGSRIC  QKKYLKSPQW  LRGLKFKKRP  WWRLDKFRLN180
NIIENGGWHF  CNLKSPKELL  YKYRNLCETN  DPYIFKEKIE  EKYLNISQIK  NKIENKLDII240
GRDQSYTKIN  IDNTYPEYLL  NNIDKYKEWI  D271

Predicted 3D structure by AlphaFold2 with pLDDT = 93.26 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MTKLYDCFSY  WDEDLLLDLR  LNILNDYVDY  FVIVEGNKTW  QNNPKKLRFD  INKFSKFKDK60
IIYIPVEDMP  DGDNPYLREN  FQRNCITRGL  DKSKNEDIIL  ISDLDEIPNP  KKISIFKKKM120
KYAVFKQNHF  YYKFNFISKT  NPFWFGSRIC  QKKYLKSPQW  LRGLKFKKRP  WWRLDKFRLN180
NIIENGGWHF  CNLKSPKELL  YKYRNLCETN  DPYIFKEKIE  EKYLNISQIK  NKIENKLDII240
GRDQSYTKIN  IDNTYPEYLL  NNIDKYKEWI  D271

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GT17(3-268)

MTKLYDCFSY  WDEDLLLDLR  LNILNDYVDY  FVIVEGNKTW  QNNPKKLRFD  INKFSKFKDK60
IIYIPVEDMP  DGDNPYLREN  FQRNCITRGL  DKSKNEDIIL  ISDLDEIPNP  KKISIFKKKM120
KYAVFKQNHF  YYKFNFISKT  NPFWFGSRIC  QKKYLKSPQW  LRGLKFKKRP  WWRLDKFRLN180
NIIENGGWHF  CNLKSPKELL  YKYRNLCETN  DPYIFKEKIE  EKYLNISQIK  NKIENKLDII240
GRDQSYTKIN  IDNTYPEYLL  NNIDKYKEWI  D271

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help