CAZyme3D

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Entry ID

Information for CAZyme ID: AFM20682.1

Basic Information

GenBank IDAFM20682.1
FamilyGH23
Sequence Length233
UniProt IDI4BTS5(100,100)Download
Average pLDDT?85.49
CAZy50 ID175680
CAZy50 RepYes, AFM20682.1
Structure ClusterSC_GH23_clus393
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID891968
KingdomBacteria
PhylumSynergistota
ClassSynergistia
OrderSynergistales
FamilyAcetomicrobiaceae
GenusAcetomicrobium
SpeciesAcetomicrobium mobile

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MILKKSLRAR  YYIMAKIVTI  FLFFSTILSI  ATPGIGEIND  EKSKENIVFH  DKIIQTLAET60
GNSPEDIIRW  QKECPTSTLR  FLSEMLPDEQ  EMIASLAKYI  RKVNPKIASK  TLWREACAFA120
YYSKKYGVPK  ELLIAVAKAE  SHFDPKATSS  KGAVGVMQVV  WDTHSALLSA  NGITSKNHLN180
DPELGIAAGA  LLLSRYIRAY  GSEHKALDRY  YGTASVRYKN  MINKNMMALE  SFL233

Predicted 3D structure by AlphaFold2 with pLDDT = 85.49 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MILKKSLRAR  YYIMAKIVTI  FLFFSTILSI  ATPGIGEIND  EKSKENIVFH  DKIIQTLAET60
GNSPEDIIRW  QKECPTSTLR  FLSEMLPDEQ  EMIASLAKYI  RKVNPKIASK  TLWREACAFA120
YYSKKYGVPK  ELLIAVAKAE  SHFDPKATSS  KGAVGVMQVV  WDTHSALLSA  NGITSKNHLN180
DPELGIAAGA  LLLSRYIRAY  GSEHKALDRY  YGTASVRYKN  MINKNMMALE  SFL233

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GH23(131-215)

MILKKSLRAR  YYIMAKIVTI  FLFFSTILSI  ATPGIGEIND  EKSKENIVFH  DKIIQTLAET60
GNSPEDIIRW  QKECPTSTLR  FLSEMLPDEQ  EMIASLAKYI  RKVNPKIASK  TLWREACAFA120
YYSKKYGVPK  ELLIAVAKAE  SHFDPKATSS  KGAVGVMQVV  WDTHSALLSA  NGITSKNHLN180
DPELGIAAGA  LLLSRYIRAY  GSEHKALDRY  YGTASVRYKN  MINKNMMALE  SFL233

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help