CAZyme3D

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Entry ID

Information for CAZyme ID: AFJ63772.1

Basic Information

GenBank IDAFJ63772.1
FamilyGT26
Sequence Length268
UniProt IDI2CAU8(100,100)Download
Average pLDDT?84.28
CAZy50 ID166823
CAZy50 RepYes, AFJ63772.1
Structure ClusterSC_GT26_clus16
EC Number(s)-
Substrates(s)-

Taxonomy

Tax ID1155777
KingdomBacteria
PhylumBacillota
ClassBacilli
OrderBacillales
FamilyBacillaceae
GenusBacillus
SpeciesBacillus velezensis

Protein Sequence:
90 < plddt <=100;
70 < plddt <= 90;
50 < plddt <= 70;
0 <= plddt <= 50;     Download help

MKENPVQKGG  RQMQTEMVHN  LSYVNGNLQE  FISYLDTYYI  AQKQGAVIAT  VNPEIGYAAA60
KDKGYHQVIS  SADFVLPDGI  GVVLMSRLMQ  HPLKSRIAGF  DVFLALLGLA  NRQSKKIFLY120
GAKQDVLDAV  LNRISKEYPN  IEVVGASNGY  EADKRFVAKQ  ISRAKPDLVF  VALGYPHQEQ180
FIYEHKHLFP  QAVSIGLGGS  FDVFSGTVKR  APEWMIKTNL  EWLYRLVTNP  WRWKRMLNIP240
KYAFTVLRNN  KSNKSLYSKQ  AEDQTKQL268

Predicted 3D structure by AlphaFold2 with pLDDT = 84.28 ; Download help

pLDDT is for per-residue accuracy of the structure, which representes the quality of the residue. A higher value indicates better prediction accuracy. More detail please see AlphaFold .

Residues were colored according to plddt ( blue-> high quality; red-> low quality ).

Full Sequence:
CAPSIF:V and CAPSIF:G =99.9;
CAPSIF:V =59.9;
CAPSIF:G =40;
Non-Binding=0;     Download help

MKENPVQKGG  RQMQTEMVHN  LSYVNGNLQE  FISYLDTYYI  AQKQGAVIAT  VNPEIGYAAA60
KDKGYHQVIS  SADFVLPDGI  GVVLMSRLMQ  HPLKSRIAGF  DVFLALLGLA  NRQSKKIFLY120
GAKQDVLDAV  LNRISKEYPN  IEVVGASNGY  EADKRFVAKQ  ISRAKPDLVF  VALGYPHQEQ180
FIYEHKHLFP  QAVSIGLGGS  FDVFSGTVKR  APEWMIKTNL  EWLYRLVTNP  WRWKRMLNIP240
KYAFTVLRNN  KSNKSLYSKQ  AEDQTKQL268

Carbohydrate binding residues Predicted by CAPSIF from 3D structure; Download help

Residues were colored according to prediction score:

Nonbinder, CAPSIF:G Predicted Binder, CAPSIF:V Predicted Binder, CAPSIF:V and CAPSIF:G Predicted Binder

CArbohydrate–Protein interaction Site IdentiFier (CAPSIF) that predicts non-covalent carbohydrate-binding sites on proteins: (1) a 3D-UNet voxel-based neural network model (CAPSIF:V) and (2) an equivariant graph neural network model (CAPSIF:G).

Details:
⋆B-Factor = 0.0 : Nonbinder.
⋆B-Factor = 40.0 : CAPSIF:G Predicted Binder.
⋆B-Factor = 59.9 : CAPSIF:V Predicted Binder.
⋆B-Factor = 99.9 : CAPSIF:V and CAPSIF:G Predicted Binder.

For more detail please see CAPSIF.

Full Sequence:
AA;
CE;
PL;
GH;
GT;
CBM;     Download structure help

dbCAN3 predicted domain(s) : GT26(69-236)

MKENPVQKGG  RQMQTEMVHN  LSYVNGNLQE  FISYLDTYYI  AQKQGAVIAT  VNPEIGYAAA60
KDKGYHQVIS  SADFVLPDGI  GVVLMSRLMQ  HPLKSRIAGF  DVFLALLGLA  NRQSKKIFLY120
GAKQDVLDAV  LNRISKEYPN  IEVVGASNGY  EADKRFVAKQ  ISRAKPDLVF  VALGYPHQEQ180
FIYEHKHLFP  QAVSIGLGGS  FDVFSGTVKR  APEWMIKTNL  EWLYRLVTNP  WRWKRMLNIP240
KYAFTVLRNN  KSNKSLYSKQ  AEDQTKQL268

Predicted CAZyme domains from dbCAN; Download help

Domains were colored according to CAZyme classification: (AA), (CE), (PL), (GH), (GT), (CBM), & (Null)

dbCAN3 server is a web server for automated Carbohydrate-active enzyme ANnotation.

Details:
dbCAN3 server integrates three state-of-the-art tools/databases for automated CAZyme annotation:
⋆HMMER search for CAZyme family annotation vs. dbCAN CAZyme domain HMM database
⋆DIAMOND search for BLAST hits in the CAZy database
⋆HMMER search for CAZyme subfamily annotation vs. dbCAN-sub HMM database of CAZyme subfamilies (derived from eCAMI classification of CAZyDB families)

For more details, please see dbCAN3.

Similarites between the same cluster seqeunces from DIAMOND; Download help