y
Basic Information | |
---|---|
Species | Manihot esculenta |
Cazyme ID | cassava4.1_003482m |
Family | GH32 |
Protein Properties | Length: 637 Molecular Weight: 71213.2 Isoelectric Point: 4.6259 |
Chromosome | Chromosome/Scaffold: 01127 Start: 398782 End: 401926 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH32 | 121 | 437 | 0 |
HFQPERNWMNGPLFHMGWYHLFYQYNPDSAVWGNITWGHAVSRDLIHWFHLPFAMVPDQWYDINGVWTGSATLLPDGQIVMLYTGDTIDSVQVQNLAYPA NLSDPLLIDWIKYPGNPVLVPPPGIETDEFRDPTTGWLGPDGKWRITIGSRVNETIGVSLVYQTTNFTTYELLEGFLHAVPGTGMWECVDFYPVAINGSL GLDTSANGPDTKHVLKASLDDTKIDHYALGTYDPVTDRWTPDNPKEDVGIGLRVDYGRYYASKTFYDQQKKRRILWGWINETDTEEDDLEKGWASVQTIP RAVLFDNKTGTNLLQWP |
Full Sequence |
---|
Protein Sequence Length: 637 Download |
MDYPTSEYNE HAPYTSLRGQ PSTAGPPIKL RRPLKVFSVT FASFIFLLSL VTLIINQSQE 60 PLPTPNKNPS PSTPKATSFA KREPRGVAEG VSAKSNPSFF SDGVSYNWTN AMFYWQRTAY 120 HFQPERNWMN GPLFHMGWYH LFYQYNPDSA VWGNITWGHA VSRDLIHWFH LPFAMVPDQW 180 YDINGVWTGS ATLLPDGQIV MLYTGDTIDS VQVQNLAYPA NLSDPLLIDW IKYPGNPVLV 240 PPPGIETDEF RDPTTGWLGP DGKWRITIGS RVNETIGVSL VYQTTNFTTY ELLEGFLHAV 300 PGTGMWECVD FYPVAINGSL GLDTSANGPD TKHVLKASLD DTKIDHYALG TYDPVTDRWT 360 PDNPKEDVGI GLRVDYGRYY ASKTFYDQQK KRRILWGWIN ETDTEEDDLE KGWASVQTIP 420 RAVLFDNKTG TNLLQWPVEE IESLRLNSTD FEEILIGPGS VVPLDIGVAT QLDIFAEFET 480 ELISDSAVEE YDCSGGAVDR SSLGPFGILV IADQTLSELT PVFFRPVNST DGTLKTYFCA 540 DETRSSKAPD VFKQVYGSTV PVLEGEKLGM RVLVDHSIVE SFAQGGRTVM TSRIYPTEAI 600 YGAARLFLFN NATSVNVKAT LKIWQLNSAF IRPFPL* 660 |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 4.0e-54 | 117 | 596 | 498 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 5.0e-74 | 117 | 628 | 527 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-88 | 127 | 440 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-147 | 121 | 437 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 121 | 586 | 478 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACJ04702.1 | 0 | 13 | 634 | 12 | 629 | invertase 2 [Cucumis melo] |
GenBank | ACQ82802.1 | 0 | 6 | 636 | 4 | 633 | vacuolar invertase [Gossypium hirsutum] |
DDBJ | BAF34363.1 | 0 | 20 | 636 | 22 | 639 | beta-fructofuranosidase [Citrus sinensis] |
RefSeq | XP_002321831.1 | 0 | 112 | 634 | 1 | 523 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002510944.1 | 0 | 1 | 636 | 1 | 636 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 99 | 634 | 1 | 544 | A Chain A, Crystal Structure Analysis Of The Hyperthermostable Pyrocoocus Woesei Alpha-Amylase |
PDB | 3ugh_A | 0 | 99 | 634 | 1 | 544 | A Chain A, Crystal Structure Analysis Of The Hyperthermostable Pyrocoocus Woesei Alpha-Amylase |
PDB | 3ugg_B | 0 | 99 | 634 | 1 | 544 | A Chain A, Crystal Structure Analysis Of The Hyperthermostable Pyrocoocus Woesei Alpha-Amylase |
PDB | 3ugg_A | 0 | 99 | 634 | 1 | 544 | A Chain A, Crystal Structure Analysis Of The Hyperthermostable Pyrocoocus Woesei Alpha-Amylase |
PDB | 3ugf_B | 0 | 99 | 634 | 1 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
---|---|---|---|
Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
fructan biosynthesis | RXN-1801 | EC-2.4.1.100 | 2,1-fructan:2,1-fructan 1-fructosyltransferase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |