y
Basic Information | |
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Species | Malus domestica |
Cazyme ID | MDP0000377084 |
Family | GH32 |
Protein Properties | Length: 643 Molecular Weight: 71553 Isoelectric Point: 4.6455 |
Chromosome | Chromosome/Scaffold: 02639128 Start: 9476 End: 13146 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 123 | 438 | 0 |
HFQPEKNWINGPLFHKGWYHLFYQYNPDSAVWGNITWGHAVSTDMIHWLYLPLAMVPDRWFDANGVWTGSATLLPDGQIIMLYTGSTNDSVQVQNLAYPA NLSDPLLLDWVKYEGNPILTPPSGIGSTDFRDPTTAWIGPDGKWRTTIGSKFNKTGISMVYTTTDFINYELHDGVLHEVPGTGMWECVDFYPVAINGSKG LDTSVNNGGVKHVLKASLDDTKLDHYAIGTYFIENETWVPDDPTIDVGIGLKYDYGRYYASKTFYDQNKERRILLGWINETDTETDDLKKRWASLHTIPR TVLFDSKTGTNLLQWP |
Full Sequence |
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Protein Sequence Length: 643 Download |
MDTTNNTSYT PLPGEDPSAA VPPPTHRRPF KGFAVIFSSV IFLLSFVTLV IHQGPGAPPK 60 TVPEQPDHHH QYRPASTSSE TRSFSVPRGK LEGVSAKSYP HFSEEASYNW TNAMFSWQRT 120 AFHFQPEKNW INGPLFHKGW YHLFYQYNPD SAVWGNITWG HAVSTDMIHW LYLPLAMVPD 180 RWFDANGVWT GSATLLPDGQ IIMLYTGSTN DSVQVQNLAY PANLSDPLLL DWVKYEGNPI 240 LTPPSGIGST DFRDPTTAWI GPDGKWRTTI GSKFNKTGIS MVYTTTDFIN YELHDGVLHE 300 VPGTGMWECV DFYPVAINGS KGLDTSVNNG GVKHVLKASL DDTKLDHYAI GTYFIENETW 360 VPDDPTIDVG IGLKYDYGRY YASKTFYDQN KERRILLGWI NETDTETDDL KKRWASLHTI 420 PRTVLFDSKT GTNLLQWPVE EIEDLRLNST EFTDVLVEAG TVVPLDIGTA TQLDIFVDFE 480 IELLDTEESV NGSSGCGDGA VDRSTFGPFG ILVIADESLS ELTPVYFRVT NSTDGDVTTY 540 FCADESRSSK APEVFKQVYG GEVPVLDGET YSARVLVDHS IVESYAQGGR TVISSRVYPT 600 EAIYGAARLF LFNNATGVNV KATLKIWQLN SAFIHPFPLD QI* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-44 | 119 | 599 | 515 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-66 | 119 | 631 | 534 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-82 | 129 | 441 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-141 | 123 | 438 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 2.0e-175 | 123 | 589 | 480 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAL05427.2 | 0 | 4 | 642 | 3 | 636 | vacuolar acid invertase [Prunus cerasus] |
GenBank | ACQ82802.1 | 0 | 76 | 641 | 63 | 635 | vacuolar invertase [Gossypium hirsutum] |
DDBJ | BAF35858.1 | 0 | 1 | 642 | 1 | 645 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 1 | 642 | 1 | 645 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002510944.1 | 0 | 2 | 641 | 7 | 638 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 108 | 637 | 8 | 544 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3ugh_A | 0 | 108 | 637 | 8 | 544 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3ugg_B | 0 | 108 | 637 | 8 | 544 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3ugg_A | 0 | 108 | 637 | 8 | 544 | A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47 |
PDB | 3ugf_B | 0 | 108 | 637 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |