LOCUS       NC_013132              13741 bp    DNA     linear   CON 26-AUG-2019
DEFINITION  Chitinophaga pinensis DSM 2588, complete genome.
ACCESSION   NC_013132
VERSION     NC_013132.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN00001914
            Assembly: GCF_000024005.1
KEYWORDS    RefSeq.
SOURCE      Chitinophaga pinensis DSM 2588
  ORGANISM  Chitinophaga pinensis DSM 2588
            Bacteria; Bacteroidetes; Chitinophagia; Chitinophagales;
            Chitinophagaceae; Chitinophaga.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Glavina Del Rio,T., Abt,B., Spring,S., Lapidus,A., Nolan,M.,
            Tice,H., Copeland,A., Cheng,J.F., Chen,F., Bruce,D., Goodwin,L.,
            Pitluck,S., Ivanova,N., Mavromatis,K., Mikhailova,N., Pati,A.,
            Chen,A., Palaniappan,K., Land,M., Hauser,L., Chang,Y.J.,
            Jeffries,C.D., Chain,P., Saunders,E., Detter,J.C., Brettin,T.,
            Rohde,M., Goker,M., Bristow,J., Eisen,J.A., Markowitz,V.,
            Hugenholtz,P., Kyrpides,N.C., Klenk,H.P. and Lucas,S.
  TITLE     Complete genome sequence of Chitinophaga pinensis type strain (UQM
            2034)
  JOURNAL   Stand Genomic Sci 2 (1), 87-95 (2010)
   PUBMED   21304681
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13741)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
            Mavromatis,K., Ivanova,N., Mikhailova,N., Sims,D., Meinche,L.,
            Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D.,
            Spring,S., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     The complete genome of Chitinophaga pinensis DSM 2588
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 13741)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
            Mavromatis,K., Ivanova,N., Mikhailova,N., Sims,D., Meinche,L.,
            Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D.,
            Spring,S., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/26/2019 00:28:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
            Annotation Method                 :: Best-placed reference protein
            Annotation Software revision      :: 4.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes (total)                     :: 7,171
            CDSs (total)                      :: 7,077
            Genes (coding)                    :: 7,009
            CDSs (with protein)               :: 7,009
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 19 of 68
            Pseudo Genes (incomplete)         :: 47 of 68
            Pseudo Genes (internal stop)      :: 11 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
            ##Metadata-START##
            Organism Display Name :: Chitinophaga pinensis UQM 2034, DSM 2588
            Culture Collection ID :: DSM 2588, ATCC 43595, LMG 13176, UQM 2034
            GOLD Stamp ID                     :: Gi02244
            Funding Program                   :: DOE-GEBA 2007
            Gene Calling Method               :: Prodigal
            Isolation Site                    :: Pine litter in Australia
            Isolation Country                 :: Australia
            Oxygen Requirement                :: Aerobe
            Cell Shape                        :: Rod-shaped
            Motility                          :: Motile
            Sporulation                       :: Nonsporulating
            Temperature Range                 :: Mesophile
            Temperature Optimum               :: 23 - 24C
            pH                                :: 7
            Gram Staining                     :: gram-
            Biotic Relationship               :: Free living
            Diseases                          :: None
            Phenotypes :: Biomass degrader, Chitin degradation
            Energy Source                     :: Chemoorganotroph
            ##Metadata-END##
            REFSEQ INFORMATION: The reference sequence was derived from
            CP001699.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4082984
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Annotation by JGI-ORNL and JGI-PGF
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotation Pipeline (PGAP)
            set; GeneMarkS-2+
            repeat_region
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Chitinophaga pinensis DSM 2588"
                     /mol_type="genomic DNA"
                     /strain="DSM 2588"
                     /type_material="type strain of Chitinophaga pinensis"
                     /db_xref="taxon:485918"
                     /note="type strain of Chitinophaga pinensis"
     gene            1..3420
                     /locus_tag="CPIN_RS15535"
                     /old_locus_tag="Cpin_3123"
     CDS             1..3420
                     /locus_tag="CPIN_RS15535"
                     /old_locus_tag="Cpin_3123"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="WP_012790767.1"
                     /translation="MEKTAHTRGSCIQFLLFLMRCSVYILIFAFTFSFSLFANNTDGQD
                     IREVKINLTVSNMKLSAVLERIQQMTPFRFVYNSSHIKATAPVSMHVSNMPVDKVLAQL
                     LNTSDFSFEQNKTQIIITRNTRSVMADMALEGDMLSLPVDTAITVKGKVKDDKGQPLIG
                     VTVGVKGKNRGVTTDATGSFSIRVENNDSLVFKTIGFSPVTVSALSNLNNITLSTSTTS
                     LNDVVVVGYGVQTRRDLTGTISTVKGADIKNLPVSDAAQAIQGRVAGVDIVRSDGSPGT
                     TPSIRIRGTGTINNSEPLIVIDGVPAASSGLSDINNNDIASMEVLKDASSSAIYGTRAA
                     NGVIIITTKKGTYNEKLNTSVNIYRGVSNVRKYLPLLTAPDLVKLKQERYTNDGLSVPA
                     IWQDPYYAVQRSDWQKALFNTGHVTNADVSIKGGSAFSNYLFSLGYYDEKGLITNTYFK
                     RFTVRINSEHRINSRLKVGENLQLTARKGNSLDTYSSQTGLIFSALRFNPAIPVKDETG
                     NYGSAQGSNELGDINNPVYTAETTDAWIKNYRILANAFAEVEIIRDLKLRLNYAFDGTL
                     SNSFSFLPKTLTQVRTRDDAELNQANTSTNQHLGEAFLSYNKTLAQKHQISLTGGVSYQ
                     KYTGSYFNAQRNGFDDESPYVLVLDNGSIVYNAAGNYYATNILASGFVRGFYSFKGKYL
                     LTATMRADGSSRFAPGNRWGYFPAVSAGWRVSDEAFFRNNVHAVSNLKLTGGWGVLGNQ
                     NVTEFQYLATVVKNRKYNFGDVPYTGIWNSSLANNAITWEKAHMTNISMEIGFLNNALN
                     GTITYFDKNTIDMLVPAAKPDLHGTSTVPDENMGRLNNHGWEFEVSYQGGKKAFTYFLA
                     ANATVLKNKVTRLYSSNSYIGSSNYGRQNQEISRTYEGQPIASFYGWKTAGLYQNQAQI
                     DNDPYIAKDGRKANIKPGDVRFVDVNNDGAITEADRVYLGDPNPRLQYGFQAGGNFKGF
                     DLNLSFTGVAGVKLYNADKMQGLDPTYSYNYYAEQLNRWHGEGTSNSVSRMTLSDNNGN
                     YRTSDRFIEKGNYFALRNVSLGYTIPAKVWGGQSNAPGIRVYVAGQNMFIITNYSGLNP
                     ELGYTDGNKQRGVDVATYPQARSITFGASMNF"
     gene            3442..4908
                     /locus_tag="CPIN_RS15540"
                     /old_locus_tag="Cpin_3124"
     CDS             3442..4908
                     /locus_tag="CPIN_RS15540"
                     /old_locus_tag="Cpin_3124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|C6Y217|8.A.46.1.3"
                     /protein_id="WP_012790768.1"
                     /translation="MTTKNRYKLLIIAAVLFTSMTSCDSILDVTPKGTYTTATYWRNQS
                     DAVNGITGIYNVLLEEDFTGHAEFTFDDPSDDQYRAGDHSEDVAIENLTYDAANAQVRF
                     GWKWKYEMINRANSALIYIPKITSIDEDIKNRCLGEARFLRAFAYWRLMLVYGEAPVVS
                     EDDVLTGNYNKPKVGIDSLRSMIQTDLLAAADLLPDSYEGADRGRVSKGSAWGLLCKLY
                     MYWEKLDQAIIYGEKVINSGKYNLAPTYVANFTPETSNNDEMLFAVQTSDTWGYSDFTT
                     YYTPREWNGWNFHQPLKQLVDEFEQNDARKAVSIMSPGDKINIGSSIATYNANMSETGY
                     HFRKFSYWKPNGGLNYSLKTPLLRTADIMLLVAEAKIRTAGAGAGDTEINKVRKRAGLG
                     DVSGAGMPAVIHERRMELSGENERHQDLMRWDKAGLLDITTYYNKPKYGRDGNILVQAR
                     NFIRPKHYYFPLPQTEIDKSNGVLKQNENY"
     gene            4982..7168
                     /locus_tag="CPIN_RS15545"
                     /old_locus_tag="Cpin_3125"
     CDS             4982..7168
                     /locus_tag="CPIN_RS15545"
                     /old_locus_tag="Cpin_3125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH89"
                     /protein_id="WP_012790769.1"
                     /translation="MQLKKIIAALLLSTAGLPAFAQLDKAATEAFLKRVVKDRATAFTC
                     EYLPADNGKDVFEIESNGSRIMLRGNNGVSVASALNYYLRNYCNSIITWNGTNLHLPAV
                     LPVVKEKEHHVTPYRYRYYINYCTFQYSMSWWNWERWQQEIDWMAMNGINMPLALTGEE
                     AIWQEVYKEMGFTDAELDKFFSGPAYFSWLWMGNIDAWGGPLPQHWKDSHKVLQQQILA
                     AERSMGMLPILPAFTGHVPPAFKDKYPNEIVKPTNWDAGFPDVYILDPNSPMFDKIGKK
                     FLEAQTKAFGTDHFYSADTFNENVPPSSDSSFLDAMSRKVYASMAAADPKAVWVMQGWM
                     FHYNASYWHQPQIRALLNAVPDDHMIVLDLYSESHPEWRNTQAYYGKPWIWNMLHNFGG
                     NTGMWGGMDAAAHDPATALHDPASGKMSGIGLTPEGIEQNPALYQLMIDNVWRDQPINV
                     DTWLQSYAKQRYGAENEAVNKAWQILYHTVYIGGPTEGAPESIIVARPTLDIAAERVKT
                     KLEYDPAKVVPAWDLFINAAAQLKPTEGFKYDLVDLTRQVLGNYASPLQQRVATAYRNK
                     DLAAFKQYSTQFIGLLDDMDMLLGTQEGFLLGKWVSDARSNGITPAEQDLYEFNAKDLV
                     TLWGDKDSPVHEYSNRQWNGLIKGFYKPRWQQFFTLLESSLKKGETADLKAFEEQVKAF
                     EWKWANGHDKYAVKPQGDAVKAAVQLHKKYRKMM"
     gene            complement(7225..7593)
                     /locus_tag="CPIN_RS15550"
                     /old_locus_tag="Cpin_3126"
     CDS             complement(7225..7593)
                     /locus_tag="CPIN_RS15550"
                     /old_locus_tag="Cpin_3126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013071407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|DUF24,DBD-SUPERFAMILY|0044258"
                     /protein_id="WP_012790770.1"
                     /translation="MHECNFKDPLSDQQCEEMINAVGDALYVIGGKWKLRIIIALSNGR
                     KRFNELQKALNGISARVLSNDLKDLELNGFVRRIERSESSTLLVEYELLEYSHTLLNVI
                     SSLNEWGTMHKKKIMHKD"
     gene            7658..8647
                     /locus_tag="CPIN_RS15555"
                     /old_locus_tag="Cpin_3127"
     CDS             7658..8647
                     /locus_tag="CPIN_RS15555"
                     /old_locus_tag="Cpin_3127"
                     /inference="COORDINATES: protein motif:HMM:PF02230.14"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_083781109.1"
                     /translation="MYISLRHYRKLKLSAMYLLKIGCISLAVLLVIIAALYSYYVYTPI
                     PKTPPLSATIRTSKIKVGELERSFLSYVPAKAPDHPALVIMLHGSGLDGKRFREWTGYS
                     FDQLADKHGFLVAYPDGYKGNWNDCRINAPFEAKQLNIDDMGFLHTLIAYYERQYNIDP
                     AKVFVFGYSNGGQMAFRLAMETPEKVAAIAAVCASLPDAATCSCTMQGATPPVLLINGT
                     ADKIIPYKGGEVTLFFKKVGIGISAPATAKHFADRNNAVEQQPETTSGTISQRTWIKEG
                     HAFVKLISISGGGHTVPQQTFRFPRLLGMTSTAYDSPSEACHFFGLDK"
     gene            complement(8653..12348)
                     /locus_tag="CPIN_RS15560"
                     /old_locus_tag="Cpin_3128"
     CDS             complement(8653..12348)
                     /locus_tag="CPIN_RS15560"
                     /old_locus_tag="Cpin_3128"
                     /inference="COORDINATES: protein motif:HMM:PF02156.13"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q3J125|4.D.3.1.5"
                     /protein_id="WP_012790772.1"
                     /translation="MGILLYSLFNPANIGNKPLYWLMMSGIIYFCLKVLHEWYHYFCIS
                     MPVRPRTKINPTVDIFTTFCAGEPYEMIIETLTAMQAITYPHTTWLCDEADDPYLIAVC
                     QRLGVRHVTRTNRKDAKAGNINNALQYATGELCVVMDPDHVPIPEFLDRVVPYFIDPEI
                     GFVQIVQAYGNIGDNIIAKGAAQQTFQFYGPMMMTMNTYGTVQAIGANCTFRRSALDAI
                     GGHAAGLAEDMHTAMQLHAKGWKSVYVPEVLTLGLVPSTLSAYYKQQLKWARGTFELLV
                     TTYPRLFRKFTWRQKLHYGTLPFHYCSGVMSFINFLVPVLALVLDIIPCQFDLLSFGII
                     SIPFLASTILVRHFVQRWVMAEEESGFHIVGGLLQIGTWWIYVLGLFYTIIRKKVPYIP
                     TPKDDKEGTSWKLFIPNGIVLLVSITAIIYGLWLDWNPYSWVMAGIAFINCLIMLFNIL
                     AGIWKGRMSNVAPQTTMEVIYHHYYAVKVRLWKFRHGVYVMMRKSAMSLTLIVCGFTSW
                     FIYEDNKPPPAYQSAHAYKEDVFYTGIFSPADSSGMTAMEHVMHYVAVKGTPFNIISCY
                     IPWGDMPACLPDDSLMNEIYSFHAVPMITWEPWAALFKDQFKDDELRNELKIMTHISNG
                     NFDGYLQQFTQRIIALRRPVFIRFAHEPDNPAYPWSTKGHNKAAEYIAAWRYVHDFFQR
                     AGARNVVWVWNPWKATAANRYFPGREYVDWLAVTMLNYGRLNPDGQWYNFSQLYQPFHT
                     QALFRSGLPVMIAETGSLAREPGQSGWLQDAFADITDHFPEVKATILFNSSFDKNTVGN
                     THVDMLNWELLEPSAPLSLLNDLQKNSAAPINRPVMLPAVNVVKHRQNTLLSDTIRGVI
                     YSKGIPWFRNHHTLTRKEVMTDLQSMQRIGINTIRRYGPGVYDHNIFSVAADLGMQIQY
                     GFWLPEPTIEQGLEEWPEAYIRSVVNTVRKNKDNNNIIGWHLGNNYWEALDDSYIAPSL
                     LYHQDQYIHWLRKLVRQIKAVDPGRPVTLDVPEGHRFSLTVQTIADAIPELSAIGLVSP
                     KDPGGGSITVTDIPYFFSGINADNYISSNRKGPVFIDAWQDAQTRDAITFNGLTDLYGH
                     YKPDAVRLAQYWHGSVDAPALPAIKILKPAKVTDPGAVLTYSALISEKGQWNLDGYFDP
                     SLHFEWYLYKTDGWENGIYMEHAGSGPVLHLHIPESPELYRLYLVAGKGQYVTTALSNL
                     NTPLIK"
     gene            complement(12392..13741)
                     /locus_tag="CPIN_RS15565"
                     /old_locus_tag="Cpin_3129"
     CDS             complement(12392..13741)
                     /locus_tag="CPIN_RS15565"
                     /old_locus_tag="Cpin_3129"
                     /inference="COORDINATES: protein motif:HMM:PF02836.15"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH158"
                     /protein_id="WP_148230575.1"
                     /translation="MTPDRIRRYLLFPCMLLLQLYGCKGDRTIRPSGKVYISRENGKYT
                     LYRNSTPFLVKGGAGYTNMSTLKAIGGNTIRTWDTANLKAILDEAAANNLAVVAGFYVP
                     ESKYLDHFYRNEKKVEAQYNAFRKVVLRYRSHPALLMWCLGNEVDFPYSPKYNRFYAVY
                     NRLLDMIHAEDPDHPVTTAMINYQFRNIMNIRLKISGLDLLSFNTFGDLKKLDEQLSDN
                     DWLWKGPFLVSEWGIYSPQESRTTAWSAPIEPTSTEKAKYYHEFYTKYLPVNNPRFLGA
                     LTFYWGYKTEITPTWYSIVDQHGAQTALLQEMESCWTNRQPLHPAPAVERLLINGKKPI
                     DNIFLSPDTIQTAELKMITTDTSRLRILWEVTDEDFDPGYSATIHVYDAGITPQNSARI
                     TFRSPSKEGPYRIYSWVYDKYGNIATANTPFYVIETKDKNTGTRIPAYAH"
ORIGIN
        1 atggaaaaaa cagcgcatac acggggctcg tgtatccaat tcttattatt tctcatgcga
       61 tgttcggttt atatattgat atttgccttt accttttcat tttctctgtt cgccaataat
      121 accgacggac aggatatcag agaagtaaag atcaacctga ctgtcagcaa tatgaagctg
      181 tcagccgttc tggaaaggat ccagcagatg acgcctttcc gttttgttta caattcctcc
      241 cacatcaagg cgactgcacc cgtaagtatg catgtcagca atatgccggt ggacaaagtc
      301 ctggcacaac tgctcaatac ctctgatttt agtttcgaac agaataaaac acagatcatt
      361 attacccgga atacccggag cgtgatggct gatatggcgc tggaaggcga tatgttgtca
      421 ctgccggtgg ataccgctat cactgtaaaa ggtaaggtaa aagatgataa aggccagccg
      481 ctgatcggtg ttaccgtagg cgtaaaaggc aaaaacaggg gtgtaactac tgacgctact
      541 ggtagcttct ctatcagggt tgaaaacaat gactcgctgg tattcaagac tatcggattc
      601 tctccggtga ctgtcagcgc gctctccaac ctgaataata ttaccctgtc caccagtact
      661 accagtctga atgacgtagt agtagtgggt tatggtgtac agacaagacg cgacctgacc
      721 ggtactatct ccaccgtaaa aggcgcagat atcaaaaacc tgccggtgag tgatgccgca
      781 caggccattc agggccgcgt agctggtgtc gatatcgttc gttcggatgg atctcctggt
      841 acgacgccaa gtatccgtat ccgtggtaca ggtactatca ataactcaga accgttgatc
      901 gtgatcgatg gtgtgcctgc tgcctcttcc ggcctgagtg atatcaataa taatgatatc
      961 gcttccatgg aagtactgaa agacgcttct tcttctgcta tctatggtac ccgcgctgct
     1021 aacggtgtaa tcatcattac cactaagaaa ggtacttaca acgaaaagct gaatacctcc
     1081 gtaaacatct atcgtggtgt atctaacgtg cgtaaatatc tgccgctgtt aactgcgcct
     1141 gatctcgtaa aactgaaaca ggaacgttat accaatgatg gtttgtccgt accagctatc
     1201 tggcaggatc catattatgc ggtacagcgt agcgactggc agaaagcatt attcaatacc
     1261 ggtcacgtga ccaatgcgga tgtgtctatc aaaggcggta gtgcattttc caactacctg
     1321 ttctcactgg gctactatga tgaaaaagga ttgatcacca atacctattt caaacgtttc
     1381 accgtacgta tcaattccga acacaggatc aacagccgcc tgaaagtagg ggagaactta
     1441 caactgaccg cccgtaaggg taattcactg gatacctatt cctcacagac gggtcttatc
     1501 ttcagcgccc tgcgtttcaa ccctgctatt cctgtgaagg atgaaacagg taactacgga
     1561 tccgctcagg gcagcaatga actgggtgac atcaacaacc cggtatatac cgctgaaaca
     1621 acagatgcgt ggattaagaa ctatcgtatc ctggcaaatg cttttgcaga agtagagatc
     1681 atcagagacc tgaaactgcg gctgaactac gcatttgacg gaacgctgag caactctttc
     1741 agcttcctgc ctaagacact gacacaggta cgtacccgtg acgatgcaga attaaaccag
     1801 gcgaatacaa gtaccaatca acacctggga gaagctttcc tgagttataa taagaccctt
     1861 gcacagaaac atcagatatc cttaacaggc ggtgtttcct accagaaata tacaggtagc
     1921 tatttcaacg cacagcgtaa tggctttgat gatgaatctc cttatgtact ggtgctggac
     1981 aatggttcca tcgtatacaa tgccgctggt aactattatg ctaccaacat cctggcatcc
     2041 ggatttgtaa gaggtttcta tagctttaaa ggcaaatacc tgctgaccgc tactatgcgt
     2101 gcagatggtt cttccagatt tgcaccgggt aaccgctggg gctatttccc tgctgtatct
     2161 gcaggatggc gtgtttctga tgaagccttc ttcaggaata acgtacatgc tgtcagcaat
     2221 ctgaaactga caggtggatg gggcgtactg ggtaaccaga acgtgaccga attccagtac
     2281 ttagctaccg tggttaaaaa ccgtaagtat aacttcggtg atgtgcctta taccgggatc
     2341 tggaattcca gtcttgcgaa caacgctatt acctgggaaa aagcacacat gaccaacatc
     2401 agcatggaga tcggattcct gaacaacgcg ctgaatggta ccatcactta ctttgacaag
     2461 aataccatcg acatgctggt acctgctgca aagccggacc tgcacggtac atctacagtg
     2521 cctgatgaga atatgggcag actgaacaac cacggttggg agtttgaagt gtcctatcag
     2581 ggtggtaaaa aagcgtttac ttatttccta gccgctaacg ccactgtact gaagaataaa
     2641 gtgaccagat tgtattccag caattcttat atcggttcgt ctaactacgg tcgccagaac
     2701 caggaaatct cccgtaccta cgaaggacag ccaatcgctt ctttctacgg atggaaaaca
     2761 gctggtctgt atcagaacca ggcacagata gacaacgatc cttatatcgc taaagatggc
     2821 aggaaagcga atatcaaacc aggtgacgta cgtttcgtag acgttaataa tgacggtgca
     2881 atcacggaag ccgaccgtgt gtatctgggt gatcctaacc cacgtctgca atacggtttc
     2941 caggcaggtg gtaacttcaa aggctttgac ctgaacctct cctttaccgg tgtagcaggt
     3001 gtaaaactgt ataacgcaga taaaatgcag ggcctggatc ccacctattc atacaattac
     3061 tatgcagaac agctgaaccg ctggcatggt gagggtacca gcaacagcgt atcccgtatg
     3121 accctgagcg ataacaatgg taactaccgt acgtcagaca ggttcattga aaaaggtaac
     3181 tatttcgccc tgcgcaacgt ttcactcgga tataccattc ctgcgaaagt atggggtggc
     3241 cagtccaatg cgcctggtat ccgtgtgtat gtagccggac agaatatgtt catcatcacc
     3301 aactactccg gtctgaatcc tgaactgggt tatacagacg gtaataaaca aagaggtgtg
     3361 gacgtagcca cctatccaca ggcaagaagt ataacatttg gtgcttcaat gaatttctaa
     3421 gcttcattta accaatgaac catgacaaca aaaaatcgtt ataaactttt aattatagct
     3481 gcggtactgt tcacttccat gacttcctgc gatagcatcc tggacgtaac accgaaaggc
     3541 acctatacga ccgctaccta ctggcgtaac cagtcagacg cagttaatgg tattaccggt
     3601 atttacaacg tactgctgga agaagatttc acgggacatg ccgagtttac ctttgatgat
     3661 ccttctgacg atcagtaccg tgcaggtgac cattctgaag atgtagccat cgaaaacctt
     3721 acctacgatg cagcaaatgc acaggtccgt ttcggctgga aatggaaata tgaaatgatc
     3781 aaccgcgcaa acagtgcgtt gatatacatt ccaaaaatca cttccatcga cgaggatatt
     3841 aaaaaccgtt gtctgggtga agcacgtttc ctgcgtgcat ttgcatactg gagactgatg
     3901 ctggtatacg gagaagcgcc ggtagtatca gaagacgatg tactgacagg taattataac
     3961 aaaccgaaag taggtataga cagtctgcgc tccatgatcc agacggacct gctggcagcg
     4021 gcagacttac tgccggatag ttatgaaggt gctgacagag gtcgtgtatc aaaaggttcc
     4081 gcatggggct tactgtgtaa gttgtatatg tactgggaaa agctggatca ggcaatcatc
     4141 tatggtgaga aagtgatcaa cagcggtaag tacaatcttg cgccgactta tgtagcgaac
     4201 ttcacaccag aaacaagtaa caatgatgaa atgctgtttg ccgtgcagac aagcgatacc
     4261 tggggttact cagatttcac tacttactat acaccgcgtg aatggaacgg atggaacttc
     4321 caccagccac tgaagcaact ggtagatgaa ttcgaacaga atgatgcgcg taaggcagta
     4381 tcgatcatgt ctcctggtga taagatcaat atcggttctt ctatcgccac atacaatgca
     4441 aacatgtcag agaccggtta tcacttccgt aaattctctt actggaaacc taacggtggt
     4501 ctgaactatt ccctgaaaac gcctttattg agaacagcag atatcatgtt gctggtagcg
     4561 gaagcgaaga tccgtaccgc tggtgcgggt gcaggtgata cagagatcaa taaagtgcgt
     4621 aaaagagcgg gcctgggcga tgtaagcggc gcaggtatgc cagccgttat ccatgaacgt
     4681 cgtatggaac tgagtggtga aaatgaacgc catcaggacc tgatgcgttg ggacaaagca
     4741 ggtctgctgg atattaccac ttactataat aagcccaaat atggcagaga tggtaatatc
     4801 ctggtgcagg cacgtaattt catcagacct aagcattatt atttcccatt gccccaaaca
     4861 gagattgata agagcaatgg tgtactgaag caaaacgaga attactaaca caactatcat
     4921 tcatcaatct gaaggaaaat ggctggtttc atccgccatt ttcctttaaa gtagtttctt
     4981 catgcaattg aaaaagatca ttgctgcctt gttgctcagc accgcgggat tacctgcgtt
     5041 tgcacagctg gacaaggccg ctacggaggc gtttttgaaa agggtagtaa aggacagggc
     5101 taccgcattt acctgcgaat acctgcctgc ggataacgga aaggatgtat ttgaaataga
     5161 aagtaacggc agccgtatta tgttgcgcgg aaacaacggc gtgagcgtag catcagcatt
     5221 gaactactat ctcagaaatt actgtaattc aatcattacc tggaacggca ccaacctgca
     5281 cctgccggct gtattgccgg ttgtgaaaga gaaagagcat catgtgacgc cgtacaggta
     5341 ccgttattat atcaactact gtacattcca gtacagcatg agctggtgga actgggagag
     5401 atggcagcag gaaatagact ggatggcgat gaatggtatt aacatgccat tggcgcttac
     5461 cggtgaagaa gcgatctggc aggaagtgta taaagagatg ggctttacgg atgcagaact
     5521 ggacaagttc ttcagcggac ccgcttattt ctcctggctc tggatgggga atatcgatgc
     5581 atggggcggt ccattgccac aacactggaa agacagccat aaggtattac agcaacagat
     5641 actggccgca gaacgtagta tgggtatgtt gccaatatta ccagccttta ccggacacgt
     5701 accgccagcc tttaaagata aatatccgaa tgagatcgta aagccgacta actgggacgc
     5761 tggtttcccg gatgtatata tcctcgatcc aaacagtccg atgtttgaca agatcgggaa
     5821 gaagttcctg gaagcacaga ccaaagcttt tggtactgat cacttttact cagcggatac
     5881 cttcaatgaa aatgtgccgc cttcgagcga ttcttcattc ctggatgcca tgagtcgtaa
     5941 ggtatatgcg tctatggcag ctgctgatcc gaaagccgtg tgggtaatgc agggatggat
     6001 gttccattat aatgcgagtt actggcatca gccacagatc agagccttgt taaatgcagt
     6061 gcctgatgat catatgatcg tactggatct gtatagtgaa agtcatcctg aatggaggaa
     6121 tacacaggct tattacggca aaccatggat ctggaatatg ctgcacaact ttggtggtaa
     6181 taccggtatg tggggtggaa tggatgccgc tgcacacgat ccggctactg cgttgcatga
     6241 tcctgcttcc ggaaagatgt cgggtattgg tctgacgccg gagggcattg agcagaatcc
     6301 tgcgctgtac cagctgatga ttgataacgt ttggagagat cagccgatca acgtagatac
     6361 ctggctgcaa tcgtatgcaa aacaacgata cggtgcagag aatgaggcag taaacaaggc
     6421 atggcagatc ctgtatcata ccgtgtatat tggcggtccg acagaaggcg cgccggaatc
     6481 gatcattgtt gcaagaccaa cactggatat cgcagcagag agagtaaaga ccaaacttga
     6541 atatgatcct gccaaagtag taccggcatg ggatctcttc atcaatgcag ccgcacagct
     6601 gaagccaaca gaaggattta agtatgacct ggtagacctg acccgtcagg tgcttggtaa
     6661 ctatgccagt ccattacaac agcgtgtagc gactgcttat cgcaataaag atctggcggc
     6721 atttaaacaa tacagcacac aattcatcgg actactggat gacatggata tgctgctggg
     6781 gactcaggaa ggatttctat tgggcaaatg ggtaagcgat gcgcgcagta atggtattac
     6841 gccggcagag caggacctgt atgagttcaa tgcaaaagac ctggtgacct tgtgggggga
     6901 taaggatagt ccggtacacg aatattcaaa ccgtcagtgg aacgggctga ttaaaggctt
     6961 ttataagcct cgttggcagc agttcttcac tttgctggag tccagcctca aaaagggcga
     7021 aacagccgat ttaaaggcat ttgaagagca ggtgaaggca tttgagtgga aatgggcgaa
     7081 cgggcatgat aaatatgccg taaagccaca gggagacgcc gtgaaagctg ccgttcaatt
     7141 acacaagaaa tatcgtaaga tgatgtaata aagattttct gttttaatct aggccgttcc
     7201 ccattcttgg gggggctatt tttttcaatc tttatgcatg atctttttct tatgcatggt
     7261 gccccattca ttcagcgagg agatgacatt cagcagtgtg tgactgtact ccagcaattc
     7321 atattctacc agcaatgtgc tggattcact acgttcaatt ctgcggacga aaccgttcag
     7381 ttcaagatct ttcagatcat ttgacaagac cctggcagaa ataccgttta aagccttctg
     7441 caattcgtta aaacgtttcc ttccattgga aagtgcgatg ataatccgca gtttccattt
     7501 tcctccgatg acatacaggg cgtctcccac agcattgatc atctcttcac attgctgatc
     7561 ggataacgga tccttaaaat tacactcgtg catagttcag gcagttactt caaggttagc
     7621 gctaaccttt aacttatcaa tacgaaagcg ggaactaatg tacataagtt tgcggcatta
     7681 caggaaatta aaactatcag caatgtattt actaaagatt ggatgtatca gcctggccgt
     7741 attactggtc atcatcgccg ctttgtacag ttactatgtc tatacgccca tacctaagac
     7801 gcccccgctc agcgcaacta tcagaacatc taaaattaag gtgggcgaac tggaacgtag
     7861 cttcctttca tatgtgcctg ctaaagcacc cgatcatccg gcacttgtca ttatgttaca
     7921 tggttccggt ctggacggga agcgtttcag ggaatggacc ggttatagct tcgatcagct
     7981 ggcagataaa catggctttc ttgtcgctta tcccgacggt tataaaggca actggaatga
     8041 ttgccgtatc aatgctcctt ttgaggcaaa acagttgaat attgacgata tgggcttttt
     8101 gcatacattg atcgcatact atgaacgtca atataatatt gatccggcaa aggtgtttgt
     8161 gtttggctat tccaacggcg gacaaatggc tttcagactc gctatggaaa cacctgaaaa
     8221 ggtagctgct atcgcagcag tgtgtgccag tttaccggat gccgctacct gctcatgtac
     8281 gatgcagggc gctactccac cagtactgct gatcaatggt acagccgata agatcattcc
     8341 ttataaggga ggagaagtaa cgcttttctt taagaaagta ggcatcggta tttctgcgcc
     8401 tgctacggca aaacattttg cagacaggaa caacgctgta gaacaacaac ctgaaaccac
     8461 atcaggaacg atctctcagc gtacatggat aaaagaagga catgcctttg taaaactgat
     8521 cagtatatcg ggtggtgggc ataccgtccc tcagcagaca ttcaggttcc caaggttgtt
     8581 ggggatgact tctacggcgt atgatagtcc gtctgaagcc tgtcacttct tcggattaga
     8641 taaataaagc gtttatttta tcagtggcgt attcagattg gaaagggcgg tcgttacata
     8701 ttgtcctttc cccgctacaa gatacagccg gtatagttcc ggcgattcgg ggatatgcag
     8761 gtggagcacc gggccgctgc ctgcatgttc catataaata ccattttccc atccatctgt
     8821 tttgtagagg taccactcaa aatgtagtga gggatcgaag tagccgtcca ggttccattg
     8881 tcctttttcc gatatcagtg cactgtaagt cagtacagcc ccgggatcag tcacctttgc
     8941 cggtttcagt atttttatcg caggtaatgc aggtgcatca acgctgccat gccagtattg
     9001 cgcaagacgt accgcatcgg gtttgtagtg gccatacagg tccgtcagcc cattgaatgt
     9061 gatcgcgtct cttgtttgcg catcctgcca ggcatcaata aaaaccggtc cttttctgtt
     9121 ggaggatata tagttgtccg cattgatacc gctgaagaag tacggtatat ctgtaacggt
     9181 aatactgcca ccgccaggat ctttgggaga tacaagtcct attgcagaga gttccggaat
     9241 ggcgtccgcg atcgtttgta cggtgagcga gaagcggtgt ccctcaggta catcgagtgt
     9301 aactggtctg ccgggatcga ctgcttttat ctgccgtacc agcttgcgca accaatggat
     9361 atactggtcc tggtgataga gcaatgaagg cgcaatgtag ctatcgtcca gtgcttccca
     9421 gtaattattc cccaggtgcc agccgatgat attgttgttg tctttgtttt tgcggacggt
     9481 attcactact gaacggatat aggcttccgg ccattcttca agcccctgct caatggtggg
     9541 ttccggtagc cagaaaccgt attgtatctg catacccaga tccgcagcaa cggaaaaaat
     9601 attgtgatca tatacacctg gaccatagcg tctgatcgta ttgataccga tacgttgcat
     9661 ggattggaga tctgtcatca cttcctttcg cgtcagcgta tgatggttcc tgaaccaggg
     9721 gattcccttt gaatagataa cacctctgat ggtatcgctc aacagggtgt tttgtctatg
     9781 cttcaccaca ttaacggcag gcagcataac cggtctgttt atcggagcag cactgttttt
     9841 ctgcagatca ttgagcagtg acaatggtgc agaaggttcc agcagttccc agttcagcat
     9901 gtctacatgg gtattgccga cagtgttttt atcgaaactg ctgttgaaaa gaatagtggc
     9961 ttttacctcc ggaaaatgat ctgtaatatc cgcaaatgcg tcctgtaacc agccgctttg
    10021 tccgggctct ctggccagag aacctgtttc tgcgatcatc accggcaatc ctgagcggaa
    10081 aagtgcctgt gtatgaaatg gctggtacaa ctgactgaag ttgtaccatt gtccgtcggg
    10141 atttagtctg ccgtaattaa gcatcgtaac agccagccag tcaacatatt ctctgccggg
    10201 gaaatacctg tttgctgccg ttgctttcca tgggttccat acccatacca catttctggc
    10261 ccctgcccgc tggaagaagt catgcacata tctccatgcg gcaatatact ctgcggcttt
    10321 gttatgtcct tttgttgacc atggataagc aggattgtcc ggctcatgtg caaagcggat
    10381 gaatacaggc cggcgcaagg caatgatacg ttgtgtgaac tgttgcaggt agccatcaaa
    10441 attgccgttg ctgatatgtg tcattatctt cagctcatta cgcagttcgt cgtctttaaa
    10501 ctgatcttta aaaagagcgg cccagggttc ccaggtgatc atcggaacag catgaaagga
    10561 ataaatctca ttcataagtg aatcatctgg cagacaggca ggcatatctc cccaggggat
    10621 gtaacaggag atgatattaa acggggttcc tttcacggca acgtaatgca ttacatgctc
    10681 catggccgtc atgccgctgg aatctgccgg actgaatatc cctgtataga agacgtcttc
    10741 tttgtatgcg tgtgctgact gataagcagg aggaggttta ttatcctcat aaataaacca
    10801 ggaggtgaaa ccacaaacga ttaatgtaag cgacatggca gacttacgca tcatgacata
    10861 cacaccgtgt ctgaacttcc agagtcttac tttaactgca tagtaatgat gatatataac
    10921 ctccatggtt gtttgcggcg cgacattact catacgacct ttccagatac ctgccaggat
    10981 attaaacagc atgatcaggc agttgataaa tgcaatgcct gccatcaccc atgaataagg
    11041 attccagtcc agccacaatc cgtatatgat ggccgtgatt gaaaccagca gaacaatacc
    11101 gttaggaata aacaatttcc agctggtacc ttctttatca tctttggggg ttggtatgta
    11161 gggtactttt ttacggataa tggtatagaa cagtcctaac acatagatcc accaggtgcc
    11221 tatctgtagt agtccgccga cgatatgaaa cccgctttcc tcttctgcca ttacccagcg
    11281 ttgtacaaaa tgtcttacca gtatcgtact ggccagaaaa ggaatactga taataccgaa
    11341 tgaaagcaga tcgaactgac aggggatgat atccagtacc agcgcgagca ccggcaccag
    11401 gaagttgata aaagacatga ctcctgagca ataatgaaaa gggagtgtac cataatgcag
    11461 tttttgtcgc caggtaaatt tccggaacaa tctgggatag gtagtgacca gtaactcaaa
    11521 cgttcctctt gcccatttca gctgttgttt atagtaagca gagagagtag aaggcaccaa
    11581 tccaagggtt agtacttccg gtacatagac tgatttccag cctttggcgt gtaattgcat
    11641 ggcggtatgc atatcttctg caagtccggc agcatgacca ccgatagcgt cgagtgcgct
    11701 gcgtcggaag gtacagttcg cgcctattgc ctgtaccgta ccgtaagtgt tcatggtcat
    11761 catcatggga ccatagaact gaaaggtttg ctgggcggcg cctttggcaa tgatattatc
    11821 gccgatatta ccatatgcct gtacgatctg cacaaatcct atctccggat caataaagta
    11881 aggcactacg cgatcgagga attccgggat cggtacgtga tccggatcca tcacaacaca
    11941 tagttcgccg gtagcatatt gtagggcatt attgatatta cctgctttgg catctttacg
    12001 gttggtcctg gtgacgtgcc gtacgcccag tcgttgacat acggctatga gatagggatc
    12061 atcggcttca tcacagagcc aggtggtatg aggataggta atagcctgca tggcggtgag
    12121 tgtttctatg atcatctcat acggctctcc ggcgcagaag gtggtaaata tatctaccgt
    12181 gggatttatt ttagtacggg gccgtacggg catggagata cagaagtagt gataccattc
    12241 gtgcagcact ttcaggcaaa agtagataat gcccgacatc atcagccaat ataggggttt
    12301 atttcctatg ttggcagggt tgaagaggct gtacaggagt attcccatac tggctatccc
    12361 ggttacaatg atgaacctaa ggatcaataa ctcaatgcgc gtaggccggg atacgcgtgc
    12421 ccgtattttt gtcttttgtt tcaattacgt aaaagggggt attggcagtt gcaatatttc
    12481 catatttgtc atagacccat gaatagatac gataggggcc ttcttttgat ggcgaacgga
    12541 aggtgatccg tgcgctgttc tgaggagtaa tccctgcatc gtaaacgtgg atggtagcgc
    12601 tgtatccagg gtcaaaatcc tcgtctgtca cttcccatag aatacggagc cgggaggtat
    12661 cagtcgttat cattttcaac tcagcagtct gtatcgtgtc aggagacaga aagatgttat
    12721 cgatcggttt cttgccattt atcagcaacc tttctaccgc cggtgccgga tgtagcggct
    12781 gtctgtttgt ccagcaggat tccatttcct gcaggagggc tgtctgagcg ccatgctgat
    12841 cgacgatgct gtaccaggta ggcgtgattt ccgttttata tccccagtaa aaggtcagtg
    12901 cgccaaggaa acgtggattg tttaccggaa ggtattttgt atagaattca tgataatatt
    12961 ttgctttttc ggtactggta ggttcaatcg gtgcgctcca tgccgtcgtc cgcgattcct
    13021 gcggagaata gatcccccat tcagagacga ggaagggacc cttccataac cagtcattat
    13081 ccgacagctg ctcgtccagt tttttcagat cgccgaaagt attaaacgat agcagatcaa
    13141 gtccggagat ctttaaccgg atgttcataa tatttctgaa ctgataattg atcatcgccg
    13201 tggtaacagg atgatccggg tcttcggcat gtatcatgtc gagcaggcgg ttataaacgg
    13261 catagaaccg gttgtattta ggactgtaag gaaagtctac ttcgttaccc aggcaccaca
    13321 tcagcaaagc agggtgggag cggtaacgta atacgacctt ccggaaggca ttatattgtg
    13381 cttccacttt cttttcatta cggtaaaaat ggtccagata cttgctttcg ggaacataaa
    13441 agcctgcaac gacggcaagg ttgttggcag cagcttcatc cagaattgct ttaagattgg
    13501 cggtatccca ggtacggatg gtattacctc ctattgcttt caacgtggac atattagtgt
    13561 aaccggctcc tccctttacc aggaacgggg tactgtttcg atataatgta tacttaccat
    13621 tttccctgga tatatacacc ttcccggatg gcctgattgt tctatcacct ttgcagccat
    13681 acagttggag tagcagcata caggggaaaa gcaagtaccg tctgatacga tctggcgtca
    13741 t
//
