LOCUS       AAXF02000051           14216 bp    DNA     linear   BCT 04-AUG-2012
DEFINITION  Bacteroides ovatus ATCC 8483 B_ovatus-MSIQ_Cont521, whole genome
            shotgun sequence.
ACCESSION   AAXF02000051
VERSION     AAXF02000051.1
DBLINK      BioProject: PRJNA18191
            BioSample: SAMN00627058
KEYWORDS    WGS.
SOURCE      Bacteroides ovatus ATCC 8483
  ORGANISM  Bacteroides ovatus ATCC 8483
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 14216)
  AUTHORS   Sudarsanam,P., Ley,R., Guruge,J., Turnbaugh,P.J., Mahowald,M.,
            Liep,D. and Gordon,J.
  TITLE     Draft genome sequence of Bacteroides ovatus (ATCC 8483)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14216)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
REFERENCE   3  (bases 1 to 14216)
  AUTHORS   Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H.,
            Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R.
            and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2007) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     Bacteroides ovatus (GenBank Accession Number for 16S rDNA gene:
            X83952) is a member of the division Bacteroidetes. In one
            comprehensive 16S rDNA sequence-based enumeration of the colonic
            microbiota of three healthy adult humans, it represents, on
            average, 0.034% of all 16S rDNA sequences and 0.071% of the
            sequences in its division (Eckburg et. al. (2005)). The sequenced
            strain was obtained from ATCC (ATCC 8483T).
            We have collected 6.9X coverage in plasmid end reads and 454 reads.
            We will be performing one round of automated sequence improvement
            (pre-finishing).
            Sequencing/Assembly: The genomic DNA was purified from liquid
            culture derived from a single bacterial colony. A hybrid sequencing
            strategy that utilized reads from both 454 GS-20 and ABI 3730xl
            sequencers was devised and implemented to generate the draft genome
            sequences.  454 reads were assembled using Newbler (454 Life
            Sciences) into 454 de novo contigs. These de novo contigs were
            converted in silico to 800 base paired reads ('superreads') with
            400 base overlaps with neighboring superreads.  Finally, PCAP
            (Huang, et al, Genome Research, 13:2164, (2003)) was used to
            assemble the super-reads and the conventional 3730xl capillary
            reads.
            This sequenced strain is part of a comprehensive, sequence-based
            survey of members of the normal human gut microbiota.  A joint
            effort of the WU-GSC and the Center for Genome Sciences at
            Washington University School of Medicine, the purpose of this
            survey is to provide the general scientific community with a broad
            view of the gene content of 100 representatives of the major
            divisions represented in the intestine's microbial community. This
            information should provide a frame of reference for analyzing
            metagenomic studies of the human gut microbiome. Further details of
            this effort are described in a white paper entitled 'Extending Our
            View of Self: the Human Gut Microbiome Initiative (HGMI)'
            (http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/HGMIS
            eq.pdf). These studies are supported by National Human Genome
            Research Institute.
            Coding sequences were predicted using GeneMark v3.3 and Glimmer2
            v2.13. Intergenic regions notspanned by GeneMark and Glimmer2 were
            blasted against NCBI'snon-redundant (NR) database and predictions
            generated based on proteinalignments. RNA genes were determined
            using tRNAscan-SE 1.23 or Rfamv8.0. Gene names are generated at the
            contig level and may notnecessarily reflect any known order or
            orientation betweencontigs.
            For answers to your questions regarding this assembly or project,
            or any other GSC genome project, please visit our Genome Groups web
            page (http://genome.wustl.edu/genome_group_index.cgi) and email the
            designated contact person.
            Annotation was added to the contigs in August 2007, and the CDS
            comments were updated in January 2008.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            Product names were updated in August 2012.
FEATURES             Location/Qualifiers
     source          1..14216
                     /organism="Bacteroides ovatus ATCC 8483"
                     /mol_type="genomic DNA"
                     /strain="ATCC 8483"
                     /type_material="type strain of Bacteroides ovatus"
                     /db_xref="ATCC:8483"
                     /db_xref="taxon:411476"
     gene            complement(1..2277)
                     /locus_tag="BACOVA_03514"
     CDS             complement(1..2277)
                     /locus_tag="BACOVA_03514"
                     /inference="protein motif:Gene3D:IPR013781"
                     /inference="protein motif:HMMPfam:IPR006047"
                     /inference="protein motif:HMMSmart:IPR006589"
                     /inference="protein motif:superfamily:IPR013784"
                     /inference="similar to AA sequence:INSD:BAD50048.1"
                     /note="KEGG: rba:RB548 2.4e-31 glgB; 1,4-alpha-glucan
                     branching enzyme K00700; COG: COG0296 1,4-alpha-glucan
                     branching enzyme"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13|GH13_10"
                     /protein_id="EDO10881.1"
                     /db_xref="InterPro:IPR006047"
                     /db_xref="InterPro:IPR006589"
                     /db_xref="InterPro:IPR013781"
                     /db_xref="InterPro:IPR013784"
                     /translation="MKDFKYIWLLLLLILNSFGACSDDDPLMPGERPSSGTDPAPEEQV
                     LHDGFNFDPAIPKADEPLTITFKAPEGSNFYGYADDLYLHSGTGANWTGAPTWGDNQNK
                     YRLKKTKDNVWSITLSSSIRHFYSVAPSTPLQTINLIVRDAEGSQQTYDYATLVEDSQN
                     GFIWEEPQKAPLPISGEEKEGIHIHSATSIMLVLYDKDSQGGHKDCVFVTGNFNNWKLD
                     SRYMMKYDETNHCWWITLEELTAGETQFQYFVYSASDGGTYLCDPYCEQALEKGVDTNF
                     PTGAQAPYVSVVSTNPQPYQWSAGEFEMKNKENPVIYELLLRDFTSSGNLAGAMEKLPY
                     LKELGIDAIELMPVQEFAGNDSWGYNTGLYFALDASYGTQNEYKAFIDACHQNGIAVIF
                     DVVYNHTNNDNPFARMYWDTFNNRPSTKNPWLNAVTPHQKYVFSPDDFNHTSEQTKAFV
                     KRNLKYLLDTYHIDGFRFDFTKGFTQKQTTGDDDLAATDPARVSVLKEYYEAVKAVKED
                     AMVTMEHFCANEETTLATEGIHFWRNMNHSYCQSAMGWKDNSDFSGLYDTTRPNQFVGY
                     MESHDEERCAYKQIEYGNGALKTNLSERLKQLSSNAAFFFTVPGPKMLWQFGEMGYDIS
                     IDENGRTGKKPVLWEYQTERKSLVDIYTKLITLRTTHSDLFNASSQFTWKVSYNDWDNG
                     RTLTLKAVNGKQLHVYANFTNASIDYTIPEGTWYLYLENGNPVEGEKKISVPAHEFRLY
                     TNFAE"
     gene            complement(2441..3904)
                     /locus_tag="BACOVA_03515"
     CDS             complement(2441..3904)
                     /locus_tag="BACOVA_03515"
                     /inference="protein motif:superfamily:IPR013784"
                     /inference="similar to AA sequence:REFSEQ:NP_812610.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EDO10882.1"
                     /db_xref="InterPro:IPR013784"
                     /translation="MACLLLASAAFTACDEDFKDWADPQSNPQEEAITAMVDITPVASM
                     KLEEQPGDSVVIASVSSIAENFSLTACNIELVAEGQILNLPSKVKDGNIKVRLTELDQK
                     VASLYKSQKSLEREVTLKLTPVVMTTNGEATSLTEYPEMQSAITPVATPAVDTEYYIVG
                     DLNSWQMDKSTATKLEVDKDNQYLFSVVVESEEKFDFKIVPGSAIEAPDAWQRALGASK
                     VIEDPDPGLLAFRDKEGADPDNLTCAGGKKMKITINVEDYTYTIKEDLPEHMYINGSPY
                     SLGWDWAVAPEMVPVTQTPGMFWSIQYYTAGDQIKFAPVRKWEGDFGYDEEILSPEAID
                     FAELTSSGGNIGIGKSGWYLVIVAVTTEGKTISFRLPEVYLLGGVINNSWNCDETTLFR
                     IPTDKTSDFISPAATVTGMARISTTAVDAGGWWKSEFTLDLANEGDGTIVYRENKNVSD
                     NLSELGYECNVKAGQKVHINFTTGKGKVE"
     gene            complement(3948..5120)
                     /locus_tag="BACOVA_03516"
     CDS             complement(3948..5120)
                     /locus_tag="BACOVA_03516"
                     /inference="similar to AA sequence:REFSEQ:NP_812611.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EDO10883.1"
                     /translation="MKNLYKLFTLTMGLLALSACEADRDSNPVLNEPDTFVLNVPAFAS
                     NNVYDLKNSESLELTCTQPDYGIPMATTYSVQISLEEIFVDAHAETNTEANYTTLGTTH
                     SSAKMEVKALEFALALGDLWSASSDEEFPTTPIPVYVRLKAELTNSGRGIAFSNVIELP
                     KVLGYKAVPPLELPSSIFINGSMAGSNWSNWVPLAAVNGMSKFFGLFYFGGTDMFKFGT
                     KEGEYIGFNDPRLTIASDAFTGSDDGFGGQNISVNVTGWYTVIMSVSIKGTDYAFTLDI
                     APGEVCLIGNAIGDWTFGDKGKFQAPTTADADFVSPVCTGGGELRMSVKVPGEDWWRTE
                     FAIPNGKIVFRENKSVIDSWSEIGPEYAINVKAGQKINLNFVQKTGSVTQ"
     gene            complement(5156..6766)
                     /locus_tag="BACOVA_03517"
     CDS             complement(5156..6766)
                     /locus_tag="BACOVA_03517"
                     /inference="protein motif:HMMPfam:IPR012944"
                     /inference="similar to AA sequence:INSD:AAB42172.1"
                     /note="COG: NOG27599 non supervised orthologous group"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8A1G2|8.A.46.1.1"
                     /protein_id="EDO10884.1"
                     /db_xref="InterPro:IPR012944"
                     /translation="MKFKYIKSIVSAALFLSLTTGITSCINDLDISPIDPQMTATFDQD
                     MYFTKLYASLGLTGQKLSEDPDIAVKDEGQSCFYRALFTNNEYGTDEMIWTWQENAGIP
                     ELTYMRWNSSHQQTEILYNRLAYNITLCNFFLDQIAGKEDATSVQQRAEARFLRSLFYY
                     YLMDTFGKAPFTEHFSKENPPQKTASELFAYIESELESIENDMSEPRQAPFGRADKAAC
                     WLLRARLYLNAEVYTGQPRWNDAITYAGKVLDPSNGYGLCGNYEQLFMADNDENPDAKK
                     EIILSIRQDGVQAKSYGGSYFLIAATQKSDMPNRGTNDPWECIRTRKALVDKFFANSED
                     IPFTEYTDNKWQNVRDVQAAAKDERALFYTTGRKAELESVGKFTDGLSFMKWSNLRSDG
                     QPAHDAKIPDTDIPFFRLAEAYLIRAEAYLRAGGANAQQNAWLDIKALRDRAKATEIPS
                     ANNLTLDYILDERARELYLEGFRRTDLIRYGYFTSSTYLWDWKGGSFEGNGVSSIYNLY
                     PIPKTETLTNTNMTQNPGY"
     gene            complement(6796..9843)
                     /locus_tag="BACOVA_03518"
     CDS             complement(6796..9843)
                     /locus_tag="BACOVA_03518"
                     /inference="protein motif:HMMPfam:IPR000531"
                     /inference="protein motif:HMMPfam:IPR012910"
                     /inference="protein motif:superfamily:IPR000627"
                     /inference="protein motif:superfamily:IPR008969"
                     /note="KEGG: shn:Shewana3_3063 7.1e-06
                     phosphatidylglycerophosphatase K01094; COG: NOG06412 non
                     supervised orthologous group"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q45780|1.B.14.6.1"
                     /protein_id="EDO10885.1"
                     /db_xref="InterPro:IPR000531"
                     /db_xref="InterPro:IPR000627"
                     /db_xref="InterPro:IPR008969"
                     /db_xref="InterPro:IPR012910"
                     /translation="MKQVKFRIVQTILPLLIGMFLSLGAYAQQITVKGHVKDAMGEPVI
                     GANVIAKGTTTGTITDFDGNFTLNVPQNSILSITFVGYKAAEIKAAPSVMVTLEDDSQV
                     LDAVVVVGYGTVKKNDLTGSVTAIKPDKISKGVTTSAQDMITGKIAGVNVISSGTPGGG
                     ATIRIRGGSSLNAKNDPLIVIDGLAMDNSGVQGLTNPLAMVNPNDIETFTVLKDASATA
                     IYGSRASNGVIIITTKKGKAGSKPQVNYEGNVSAGILQKTIDVMDANEFKGYVSKLYGE
                     GNAPSPFGEANTDWQKEIFQTAVSTDHNVTVSGGLKNMPYRVSFGYTNQNGILMTSNFE
                     RYTASVNLTPSFFKDHLKFNINAKMMWANQRYADDGAIGAALTMDPTQPVYDSSDMYKN
                     FGGFYQPTSDGSSYNDPEWPLTLESNSTANPVSLLKLKKHTSRNTSFISNVEVDYKFHF
                     LPDLHIHANVGGDYSEGKEKNVNSPYAPGSYYYGWNGTDYGYKYNLSVNAYAQYSKEIG
                     DHYVDVILGGEEQHFHYTGYKVGQGTNPLTGEAYNPNLRSQTAWGHHSTLVSYFARVNY
                     TLLSRYLLTATFRQDGSSRFSKDNRWSSFPSVALGWKLKEENFLKNVEVLSDLKLRLGW
                     GITGQQNLGDDYDFPYMALYRVNAAGGYYPFGDTYYGTMRPKAYNEDLKWEETTTYNAG
                     FDFAFLNGRISGSMDYYYRKTDDLINTVKIAAGTNFNTQLISNIGSLKNTGLEFTINAK
                     PIVTKDFVWDLGYNITWNKNEITKLTGGDDSNYYVETGGVSTGISGATCQVQKVGYPMN
                     SFFVYQQVYDKDGKPIENMFVDRNGDGVINASDKYIYKKPAADVLMGLTSKFTYKNWDL
                     SFALRASLNNYVYNDVLASKSSVGKGGIFNHGYYSNRPTAAVNLGFEGKGDYYLSDRFV
                     ENASFLRCDNITVGYSFKNLLKSQAYKGINGRIYGTVQNPFVITKYTGLDPESVISSGN
                     DAGVAGIDRNIYPHPITILFGLSLQF"
     gene            complement(9949..12165)
                     /locus_tag="BACOVA_03519"
     CDS             complement(9949..12165)
                     /locus_tag="BACOVA_03519"
                     /inference="protein motif:superfamily:IPR012338"
                     /note="KEGG: pha:PSHAa2848 2.7e-129 alpha-glucosidase
                     K01187; COG: NOG06228 non supervised orthologous group"
                     /codon_start=1
                     /transl_table=11
                     /product="GH97"
                     /protein_id="EDO10886.1"
                     /db_xref="InterPro:IPR012338"
                     /translation="MKKKKFFSIIAFLCISFIANAQQKLTSPDGNLVLTFQVNKEGAPT
                     YDLTYKGKVVIKPSTLGLELKKEDNTRTDFDWVDRRDLTKLDSKSNLYNGFKLKDAQTT
                     TFDETWQPVWGEEKEIRNQYNELAVILFQPMNDRSIVVRFRLFNDGLGFRYEFPQQKSL
                     NYFVIKEEHSQFAMAGNHIAYWIPGDYDTQEYDYTISRLSEIRGLMQQAITPNSSQTPF
                     SPTGVQTALMMKTDDGLYINLHEAALIDYSCMHLNLDDKNMIFESWLTPDAKGDKGYMQ
                     TPCNSPWRTIIVSDDARNILASRITLNLNEPCKIADAASWIKPVKYIGVWWDMITGKGS
                     WAYTDELTSVKLGVTDYSKTKPNGKHSANTANVKRYIDFAAANGFDAVLVEGWNEGWED
                     WFGNSKDYVFDFLTAYPDFDVQEIHRYAASKGIKMMMHHETSASVRNYERHLDKAYQFM
                     VDNGYNSVKSGYVGNIIPRGEHHYGQWMNNHYLYAVKKAADYKIMVNAHEATRPTGICR
                     TYPNLIGNESARGTEYESFGGNKVYHTTILPFTRLVGGPMDYTPGIFETHCNQMNPANN
                     SQVRSTIARQLALYVTMYSPLQMAADIPENYERFMDAFQFIKDVALDWDKTIYLEAEPG
                     EYITIARKAKGTDDWYIGCTAGENGHDSQLTFDFLEPGKQYVATVYADAKDADWKDNPQ
                     AYTIKKGILNNKSKLNLHAANGGGYAISIKEVKNKSEVKGLKRL"
     gene            complement(12363..14216)
                     /locus_tag="BACOVA_03520"
     CDS             complement(12363..14216)
                     /locus_tag="BACOVA_03520"
                     /inference="protein motif:Gene3D:IPR013780"
                     /inference="protein motif:Gene3D:IPR013781"
                     /inference="protein motif:HMMPfam:IPR006047"
                     /inference="protein motif:HMMSmart:IPR006589"
                     /note="KEGG: sru:SRU_2409 3.5e-129 glycosyl hydrolase,
                     family 13, putative K00701; COG: COG0366 Glycosidases"
                     /codon_start=1
                     /transl_table=11
                     /product="GH13"
                     /protein_id="EDO10887.1"
                     /db_xref="InterPro:IPR006047"
                     /db_xref="InterPro:IPR006589"
                     /db_xref="InterPro:IPR013780"
                     /db_xref="InterPro:IPR013781"
                     /translation="MKRNLLFAILLLLLSGYHQAFAATNIKKVAPTFWWAGMKNPELQI
                     LLYGDGISSAEVSISSNDITLQDVVKQENPNYLILYLDLSKAIPQHFDILLKQGKKQTK
                     IPYELKQRKENASAVEGFNSSDVLYLIMPDRFANGNPSNDIIPGMLEANIDRNEPFARH
                     GGDLKGIEKHLDYIADLGVTSIWLNPIQENDMKEGSYHGYAITDYYQVDRRLGSNEEFR
                     NLVKEANAKGLKVVMDMIFNHCGSNNYLFKDMPAKDWFNFEGNYMQTSFKTATQMDPYT
                     SDYDKKLAIDGWFTLTMPDFNQRNRHVATYLIQSSIWWIEYAGINGIRQDTHPYADFEM
                     MAHWCKAVNDEYPSFNIVGETWLGSNVLISYWQKDSKLAYPKNSYLPTVMDFPLMEEIN
                     KAFDEETTEWNGGLFRLYEYLSQDIVYADPMSLLTFLDNHDTSRFYRSEEDTKNLNRYK
                     QALTFLLTTRGIPQIYYGTEILMAADKANGDGLLRCDFPGGWQNDAKNCFEATNRTPQQ
                     NEAFSFMQKLLQWRKGNEVIAKGKLKHFAPNKGIYVYERKYGNQSVVVFLNGNDREQTI
                     DLHPYQEILSTSSAHDLLTDKKIELRNELTLPSRGIYLLAF"
ORIGIN
        1 ttattcggca aagttggtgt ataagcggaa ctcgtgtgca ggaacactga tctttttttc
       61 tccctccacg ggattcccat tttccagata tagataccat gtaccttcag gaatagtata
      121 atcgatagat gcattggtga aattcgcata cacatgaagt tgtttcccat tcacagcttt
      181 caatgtcagt gtacgtccat tatcccaatc attataactg actttccagg taaattgaga
      241 agaagcgttg aataaatcgg agtgtgttgt ccgtagagtg attagttttg tataaatatc
      301 taccaagctt ttacgctctg tctgatattc ccaaaggact ggtttctttc cggtacgtcc
      361 attttcgtct attgaaatat cataccccat ttcgccgaat tgccaaagca ttttcggacc
      421 tggaaccgta aagaagaatg cggcgttgga tgataactgt ttgagccgtt cggacaggtt
      481 ggtttttaag gctccatttc cgtattcaat ctgtttatag gcacaccgct cttcgtcatg
      541 actttccata tagcccacaa attgattcgg acgggtggtg tcatataatc cggagaaatc
      601 actattatct ttccagccca tagccgactg acaataagaa tggttcatat tcctccaaaa
      661 atgaattcct tctgtggcta aagttgtttc ttcatttgca cagaaatgtt ccatggtaac
      721 cattgcatct tctttgacag cttttacggc ttcgtagtat tctttcagta ctgatacacg
      781 agcagggtcg gttgccgcca aatcatcatc acctgttgtc tgtttctgtg tgaaaccttt
      841 ggtgaaatcg aaacggaaac cgtcgatgtg gtaagtatct aacagatatt tcaggttgcg
      901 tttgacaaaa gcttttgttt gttctgaagt gtgattgaaa tcatccggtg aaaaaacata
      961 cttctgatgc ggtgttacag cgttcagcca tggattctta gtggaagggc ggttattaaa
     1021 cgtatcccag tacatacggg caaacggatt atcattgttc gtatggttgt agactacatc
     1081 gaaaataacg gcgattccat tctgatggca ggcgtcaata aaagctttat attcattttg
     1141 agttccatac gaggcatcca gtgcaaagta tagtccggtg ttgtatcccc aactgtcatt
     1201 acctgcaaat tcttgtacag gcatcagttc gatagcatct attcccagtt cttttaagta
     1261 aggcagcttt tccattgctc ctgccagatt tccactggaa gtaaaatccc gcaacaagag
     1321 ctcatagata accggatttt ctttattctt catctcaaat tctccggcac tccattgata
     1381 gggttgtggg tttgtgctta cgactgatac gtaaggtgct tgtgcgccag tgggaaagtt
     1441 ggtatccact cccttttcca atgcttgctc acaataagga tcgcacaagt aagtaccgcc
     1501 atccgaagca ctgtacacaa agtactggaa ctgtgtttca ccggcagtca attcctccag
     1561 agtgatccac cagcaatggt ttgtttcgtc atatttcatc atgtagcgac tatccagttt
     1621 ccagttgttg aaattacctg ttacaaatac acaatcttta tgaccgccct gactgtcctt
     1681 atcatataat acaagcatta tcgaagtggc cgaatggata tgaataccct ctttttcttc
     1741 tccggatatg gggagagggg ctttttgcgg ttcttcccag ataaatccgt tttgactatc
     1801 ttctactaaa gttgcgtaat cgtaggtttg ttggctacct tccgcatcac gtacaatcag
     1861 gttaatggtc tgtaagggag tagatggagc aacagaatag aaatggcgga tagatgagga
     1921 aagggtgata ctccacacat tgtcctttgt ctttttcagt ctgtatttat tctgattatc
     1981 tccccatgta ggtgcacctg tccagtttgc tcctgttccg gagtgtaagt acaagtcgtc
     2041 agcgtaacca tagaagttac ttccttcggg tgctttgaag gtgatagtca gtggttcatc
     2101 cgctttagga atagccggat caaaattaaa tccgtcgtgg agaacttgtt cttccggggc
     2161 aggatcagtt ccggacgaag gacgctcgcc cggcatcaac ggatcatcat ctgaacaagc
     2221 tccaaagctg ttgagtatca gcaataaaag gagccaaata tatttaaagt ctttcataag
     2281 ccttatttta gaattcttct tccaatgtag tggtactata tttagctttg agtgtgtcag
     2341 atttcgatca tatctcaaat ctcctctttc ccgaagaggg agacttgaga tatgatcgta
     2401 attgattcta ctcgttgtgc tgtttaagag tgtctgatag ttattcgact ttacctttgc
     2461 ctgtcgtaaa gttaatatgg actttctgac cggcttttac attacattcg tagccaagtt
     2521 cgcttagatt atccgatacg ttcttattct cacggtaaac gatagtacca tcgccttcat
     2581 tagccagatc aagtgtaaat tcgctcttcc accagcctcc ggcatctaca gccgtagtag
     2641 aaatacgtgc catgcctgta acggttgctg ccggtgatat gaagtcgctt gttttatcgg
     2701 tcggaatcct gaacaaggtc gtttcatcgc aattccacga attgttaatc acacctccca
     2761 acaggtaaac ttccggcaaa cggaatgaaa ttgtttttcc ttctgtagtc actgcgacaa
     2821 ttaccagata ccatccggat ttaccaatgc caatattgcc tcctgaggat gttaattcgg
     2881 cgaaatcaat tgcttccgga gataagattt cttcgtcata accaaagtct ccttcccatt
     2941 ttctaaccgg agcaaacttg atttggtctc ccgccgtata atattgtata ctccagaaca
     3001 ttccgggagt ctgtgtgaca ggaaccattt caggagctac tgcccaatcc cacccaagac
     3061 tgtacggaga tccattgatg tacatgtgct cgggtagatc ctccttgatt gtgtatgtat
     3121 aatcttctac attaatcgta attttcattt tctttccgcc ggcgcaggtg agattatccg
     3181 ggtcggctcc ttctttatcc ctgaaagcca ataatcccgg atccggatct tctattactt
     3241 tggaagcacc caaggctctt tgccaggcat ccggagcttc tatcgcactt cccgggacaa
     3301 tcttgaaatc aaacttctct tccgactcga cgactactga aaagagatac tgattgtctt
     3361 tgtctacttc gagctttgtg gctgtcgatt tgtccatctg ccagctgttt agatcaccta
     3421 ctatatagta ttccgtatcc acagccggag ttgcgaccgg agtgatggca gattgcattt
     3481 ccgggtattc agtcagtgaa gttgcttcac cgttggttgt catgactacc ggtgtcagct
     3541 ttaaagtgac ttcgcgctct aaagattttt gtgacttata caaacttgcc actttttggt
     3601 caagttctgt caacctgact tttatattcc catcttttac ttttgacggt agatttaata
     3661 tctgcccttc ggcaactaat tctatattac aagctgtgag tgagaagttt tctgcaatcg
     3721 aagaaacaga ggcgattacc acagaatcgc ccggctgttc ttcaagtttc atactggcaa
     3781 ccggagttat atctaccatt gcagttatag cttcttcctg gggattggac tggggatcag
     3841 cccagtcctt aaaatcttca tcgcaagcgg tgaaagctgc cgaagcaagc aacagacacg
     3901 ccatatatga taatcttttc atgatattca gttattaaag ttaacgatta ttgagtaacg
     3961 gaacctgttt tctgaacaaa gttcagattt attttctgcc ctgccttaac gtttatcgca
     4021 tattcaggac ctatttccga ccaactgtca atcacacttt tgttttcacg gaacacgatt
     4081 tttccattag gtatagcgaa ctccgtacgc caccagtctt ctcccggaac tttgacggac
     4141 atacgcaatt ctccgcctcc ggtgcatacc ggtgaaacaa agtctgcatc tgccgttgtt
     4201 ggtgcttgga atttgccttt atcgccgaat gtccaatctc ctatagcatt gcctatcaga
     4261 caaacttcgc ccggagcaat atccagcgta aaggcataat cagtcccttt gatgcttaca
     4321 gacatgatca ctgtatacca gcctgtgaca ttaacgctga tattttgtcc gccaaatcca
     4381 tcatccgatc cggtgaatgc atccgaggct attgtcagac gtggatcgtt gaatccgatg
     4441 tattctcctt ctttagtacc gaacttgaac atatcagtgc ctccgaaata gaataaaccg
     4501 aagaacttgg acattccgtt tactgctgcc aaaggcaccc agttgctcca attactacct
     4561 gccatcgagc cattgataaa tatactggaa ggtaattcga gaggcggtac agctttatat
     4621 cccaatactt tgggaagttc aattacattc gaaaaagcga tgccacgacc actattggtc
     4681 agttccgctt tcaggcgtac ataaacgggt atgggagtgg tcggaaattc ttcatcggag
     4741 gatgcactcc acaagtctcc cagtgccaat gcaaattcta aagctttcac ttccattttg
     4801 gcagaagaat gggtagtacc caaagttgta taattagctt ccgtatttgt ctctgcatga
     4861 gcgtcaacga aaatttcttc cagtgaaatt tgtactgaat aagtggttgc catcggaatc
     4921 ccgtaatccg gttgtgtaca ggttaattcc aatgattcgg agtttttaag gtcgtacacg
     4981 ttgttggaag caaaagctgg cacattcagt acgaacgtat ccggttcgtt caacacaggg
     5041 ttgctgtcac ggtctgcttc acaggcggac agagctagta gccccattgt caatgtaaat
     5101 agtttatata ggtttttcat tgttttcagt ctttaaaacg tgaataaagg ttctatcaat
     5161 atcccggatt ctgagtcata tttgtattcg tcagcgtttc tgttttcggt atcggataga
     5221 gattatagat cgaactcaca ccgtttcctt caaaggaacc gcctttccaa tcccagaggt
     5281 aggtgcttga ggtgaaatag ccataacgga tcaaatctgt gcgtctgaat ccttccagat
     5341 acaattcgcg ggcacgttca tccagaatat aatctaatgt caggttattg gcagagggta
     5401 tttctgttgc tttggcacgg tctctgaggg ctttgatatc caaccaggca ttttgttgtg
     5461 catttgcgcc gcctgctctc aaataggctt ccgcgcgaat cagataagct tccgccaaac
     5521 ggaagaacgg aatgtccgta tcaggaattt tagcatcgtg agcgggttgt ccgtccgagc
     5581 gcagattact ccatttcata aatgaaagtc cgtcggtgaa tttacctaca ctttccagtt
     5641 cggctttgcg accggttgta tagaacaagg cgcgttcatc ttttgcagca gcctgcacat
     5701 ctctcacatt ctgccattta ttatcggtat attcggtgaa aggaatatcc tcactgtttg
     5761 cgaagaactt gtcaaccagt gctttgcgtg tacggataca ttcccaaggg tcatttgttc
     5821 cgcgattcgg catgtcgctc ttttgcgttg cagcaatcag gaagtaactt cctccgtaac
     5881 tctttgcttg tacaccatcc tgacggatag aaaggatgat ttctttttta gcatcgggat
     5941 tttcatcatt gtctgccata aaaagttgtt cgtaatttcc acacaatccg tatccattgg
     6001 agggatcgag cactttgccg gcataggtga tagcatcatt ccaacgcggt tgtccggtgt
     6061 atacttcggc attgagatac aaacgggcac gcagcaacca gcaagcagct ttgtcggcac
     6121 gtccgaaagg agcttgacgc ggttcgctca tatcattttc gatgctttcc agttcgcttt
     6181 caatataagc gaacagttcg gaggccgtct tttgaggagg attctctttg gagaagtgtt
     6241 cggtgaaagg agcttttccg aaggtgtcca tcagataata gtaaaataag gaacggagaa
     6301 aacgggcttc ggcacgttgc tgaacggagg tagcatcctc ttttccggcg atctgatcca
     6361 ggaagaaatt gcagagtgtg atattatatg ctaaacgatt atataatatt tctgtctgct
     6421 gatgagagga gttccaacgc atataagtta gttcggggat accggcattt tcttgccagg
     6481 tccatatcat ctcatcagtg ccatattcat tgttagtgaa aagagcgcga tagaaacagg
     6541 attgaccttc gtcctttact gcgatatccg gatcttcgct caatttttgt ccggtaagtc
     6601 ccagtgaagc gtatagtttg gtaaagtaca tatcttggtc gaaggtggca gtcatctgag
     6661 gatcgattgg agatatatcc aggtcattga tacaggaggt aattccggtg gtcaggctaa
     6721 ggaacaacgc tgctgaaact attgatttta tatatttgaa cttcataata gtctactttt
     6781 taataaatta caatcttaga actgaagact tagcccgaac aggatagtga tcggatgagg
     6841 ataaatgttc ctgtcaatac ctgctactcc ggcatcattt cctgaactga taaccgattc
     6901 aggatcgagt cctgtatact tggtgataac gaagggattc tgtacagtac cgtaaatacg
     6961 gccgttgata cctttataag cctgggactt cagtaaattt ttgaagctat aacctactgt
     7021 tatgttatca caacgcaaga atgaagcatt ctcaacgaaa cgatcggaca gataatagtc
     7081 tccctttcct tcaaatccca gattcacggc agctgtcggg cggttggaat agtatccatg
     7141 attaaagatt cctcccttac cgacactgga tttactggcc agtacgtcat tgtacacata
     7201 gttattcagg ctggcacgga gggcaaagct taaatcccaa tttttataag tgaatttgga
     7261 agtcagtccc atcaatacgt cagcggcagg ttttttatag atatatttgt ccgaagcgtt
     7321 gatgacacca tctccgttac ggtctacgaa catgttttca atcggttttc catccttatc
     7381 atacacttgt tggtaaacga agaatgagtt catcggataa cctactttct gtacctggca
     7441 ggttgcaccg ctaataccag tagatacacc tccggtttct acatagtaat tagaatcatc
     7501 tcctccggtc agtttagtga tttcattttt gttccaagtg atgttatatc ccaagtccca
     7561 gacaaaatct ttggttacaa taggtttggc attgatggtg aattcaagcc ccgtattttt
     7621 taatgaaccg atgttgctga tcagttgggt attaaagtta gtccccgccg caatctttac
     7681 ggtattgatt aaatcgtcgg ttttccggta atagtaatcc atcgagccgg agatacgtcc
     7741 gttcaggaaa gcaaagtcga aacctgcgtt ataagtcgtt gtctcttccc atttcaggtc
     7801 ttcgttataa gctttaggac gcatagtgcc ataataggta tccccgaaag gataataacc
     7861 tccggctgcg ttgacgcggt acagtgccat ataagggaaa tcataatcgt cgcctaagtt
     7921 ctgctgaccc gtgatacccc atccgaggcg gagtttcaga tctgacaata cttctacgtt
     7981 tttcaggaaa ttttcttctt tcagtttcca tcccaaagca acagaaggga agctactcca
     8041 acggttgtct ttagagaaac gggaagaacc gtcctggcgg aaagtggctg tcagcaaata
     8101 acggctaagt aatgtataat ttacgcgggc aaaataagaa actaatgtac tgtgatgtcc
     8161 ccaggctgtt tgtgagcgca gattcggatt gtaggcttcg cctgttaacg gatttgttcc
     8221 ttgtccgact ttatatccgg tatagtggaa atgttgctct tcaccaccca gtattacgtc
     8281 gacataatga tccccgattt ctttggaata ttgtgcatag gcattcaccg acaggttata
     8341 tttgtagccg tagtcagtcc cgttccaacc atagtaataa gagcccgggg cataaggtga
     8401 atttacattc ttttccttac cttccgagta gtcaccgcct acgttagcat ggatatgcag
     8461 gtcagggagg aagtggaatt tatagtctac ttctacattg ctgataaaac tggtgtttct
     8521 ggaagtatgt ttctttagtt tcagtaggga aacagggttg gcggttgaat tactttccag
     8581 tgtaagcggc cattccggat cgttatatga actaccgtca ctggtcggtt ggtagaatcc
     8641 accgaagttt ttgtacatgt ccgaagagtc atatacaggt tgggtcgggt ccatggtcaa
     8701 agctgcgccg atagccccgt cgtcagcata acgttggttg gcccacatca ttttagcatt
     8761 gatattgaac ttcaggtgat ctttaaagaa agagggtgtc agattgacgg aggcggtata
     8821 acgctcgaaa ttagatgtca tcaggatccc attctgattg gtgtaaccga aagaaacacg
     8881 ataaggcata tttttcaaac caccgcttac agtcacgtta tggtctgtac taacggctgt
     8941 ctggaaaatt tctttctgcc agtcagtgtt tgcttcaccg aaggggcttg gagcatttcc
     9001 ttcaccataa agtttggata cgtatccttt gaattcattg gcatccatta cgtctattgt
     9061 cttttgcagt ataccggcgg aaacgttacc ttcatagttc acctgtggtt tgctgcctgc
     9121 tttccctttc tttgtagtga tgataatcac accattagaa gcacgtgaac catagatggc
     9181 ggtggccgaa gcatctttca aaacagtgaa agtttcgatg tcattcggat ttaccattgc
     9241 caaaggatta gtcaaacctt gcacaccgct attatccatt gccagtccgt cgataacgat
     9301 caacggatcg tttttggcgt tcaatgatga accaccgcga atacgaatcg tagctcctcc
     9361 acccggagta ccgctggaaa ttacattcac accggctatt ttacctgtta tcatatcctg
     9421 tgcgctggtg gttactcctt tgctgatttt atccggcttg atggcagtaa ctgatcccgt
     9481 caagtcgttt ttctttactg ttccgtaacc tacaacgact acggcgtcca atacttgaga
     9541 gtcatcttcc agtgtgacca ttaccgaagg agctgcttta atttctgctg ctttgtaacc
     9601 gacgaatgta atggacagaa tagaattttg aggaacattc aatgtgaagt taccgtcaaa
     9661 gtcagtgatg gtacccgttg tggtaccctt ggcaatgaca ttggcgccaa tcacgggttc
     9721 gcccatagca tctttcacat gtcctttgac tgtgatttgc tgtgcataag cgcccaatga
     9781 taaaaacatc cctattaaca aggggagaat cgtttgaacg attctaaatt taacttgctt
     9841 catgcattta aaaaattaaa gttaacaata tagtaatatt atttctctct cttaaaagtt
     9901 tctttaaaaa tacctattga aaacactttt tttaagacta tcctttattt atagtctttt
     9961 caatccctta acctctgatt tgtttttcac ctctttgata ctgattgcgt atccaccgcc
    10021 attggcagcg tgcagattca gtttgctttt attattcaag ataccttttt tgatggtgta
    10081 tgcctgcggg ttatctttcc agtcggcatc tttggcatct gcgtatacgg tagcgacgta
    10141 ttgttttccc ggttccagga aatcgaacgt taattgggag tcgtggccgt tttcaccggc
    10201 tgtgcagcct atgtaccagt cgtccgttcc ttttgctttg cgggcaattg tgatgtattc
    10261 tcccggttct gcttccagat agattgtttt gtcccagtcg agagcgacgt ctttgataaa
    10321 ctggaaagca tccataaagc gttcgtagtt ttccggaata tcggcagcca tctgcaaagg
    10381 gctatacatg gtgacataca atgccagttg gcgtgcaatg gttgaacgta cctgggagtt
    10441 gtttgccgga ttcatctggt tacagtgagt ctcgaagatg ccgggagtgt aatccatcgg
    10501 accaccgacc aggcgtgtga aaggcagaat cgtcgtgtgg tatactttat ttcctccaaa
    10561 tgattcatat tctgttccac gtgcggattc attaccgatc agattgggat atgtacggca
    10621 gataccggtt ggacgggttg cttcgtgtgc attgaccatg attttataat cggcggcttt
    10681 tttcacggca tacaggtaat gattattcat ccattgaccg taatgatgtt ctccgcgtgg
    10741 aatgatatta cccacataac cgcttttaac cgagttatat ccattgtcta ccatgaattg
    10801 gtaagcttta tccaggtgac gttcgtagtt gcgaacagat gcagaagtct catggtgcat
    10861 catcatttta attcctttgc ttgcggcata acgatggatt tcctgtacgt cgaaatcagg
    10921 atatgcagtc aggaagtcaa atacatagtc tttgctgttg ccgaaccagt cttcccatcc
    10981 ttcattccaa ccttctacca gcactgcatc aaatccgttg gctgcggcaa agtcgatgta
    11041 acgtttcacg ttagctgtat tggcagaatg tttgccgttc ggtttggttt tggagtagtc
    11101 tgtaacgcca agtttcacgc tggtcagttc atctgtataa gcccaggaac cttttccggt
    11161 aatcatgtcc caccatacgc ctatatattt caccggttta atccaggagg cggcgtctgc
    11221 aatcttacaa ggttcgttca ggttgagggt gatgcgggag gcgagaatgt tacgtgcatc
    11281 atcactgacg atgatcgtac gccacggaga gttgcacgga gtctgcatgt aacctttatc
    11341 cccttttgca tccggtgtca gccaggattc gaatatcatg tttttgtcgt ccagattgag
    11401 gtgcatacaa gaataatcaa tcaaagcggc ttcgtgcaga ttgatgtata aaccatcatc
    11461 tgttttcatc atcagtgccg tctgtacacc tgtcggtgaa aatggggttt gagaagaatt
    11521 tggagtgatc gcctgttgca tcaaacctct gatttccgat agacgggaga tggtgtagtc
    11581 gtactcttga gtgtcgtaat cgcccggtat ccagtatgcg atatggttgc ctgccatggc
    11641 aaattgggaa tgttcttcct tgataacgaa gtagttcagt gatttctgtt gcggaaattc
    11701 ataacggaat cccaaaccat cattgaacag gcggaagcga acaacgatgg aacggtcgtt
    11761 cattggctga aacagaatga ctgccagttc gttatactga ttgcggattt ccttttcttc
    11821 tccccagact ggttgccagg tttcgtcaaa ggtagtcgtt tgcgcatctt tcagcttgaa
    11881 gccattataa agattggatt tagaatcgag cttggtgagg tctctgcggt ctacccagtc
    11941 aaagtcggtt ctggtattat cttctttttt caattccagt ccgagagtgc tcggcttaat
    12001 taccaccttg cctttgtaag tcaagtcgta ggttggtgcg ccttctttgt taacctgaaa
    12061 ggtcagaacc agattgccat ccggtgaggt cagtttctgt tgtgcattcg caatgaatga
    12121 gatgcaaagg aatgcgatga tcgaaaaaaa ctttttcttt ttcattctat ttatggtatt
    12181 aaattgtaag ctaaccgata tttctattcc tgtttgtttc ggatattgca aatgtagcaa
    12241 ccaccttcac ttttttgctt gattagaata aaaatacaag tttgtataaa tgctagtatc
    12301 ttgttttata attgtttatc tatctgattt cattggataa atataagtcg tagtgaactt
    12361 gcttagaagg ctaaaaggta tatcccacgg cttggaagtg ttaattcatt tcttaattca
    12421 atctttttgt ctgtgagcaa atcatgagca gacgaggtgg agagtatttc ctgataagga
    12481 tgtaagtcaa tagtctgttc ccggtcgttt ccattcagaa agacaacgac tgattggtta
    12541 ccgtatttgc gttcataaac ataaatgcct ttattgggtg cgaaatgttt tagtttgcct
    12601 ttggcgatga cttcatttcc ttttctccat tggagcagtt tttgcataaa agagaaggct
    12661 tcattttgtt gcggagtacg gttggttgct tcaaaacagt ttttggcgtc attctgccaa
    12721 cctcccggaa agtcacatcg gagtaatccg tctccatttg ctttgtcggc tgccatcagg
    12781 atttcagttc cataatatat ttgtggaatt ccacgggtag tcagtaagaa tgtcaaggct
    12841 tgtttatatc ggttcaggtt cttggtatct tcctccgaac ggtagaaacg ggaagtatcg
    12901 tgattatcga ggaaggtcag cagactcatt ggatcggcgt agacaatatc ctgtgagagg
    12961 tattcgtaga gtctgaaaag tcctccgttc cattcggtgg tttcttcgtc gaaagcttta
    13021 tttatttctt ccatcagtgg gaaatccatg acagtaggaa gataactgtt tttcggataa
    13081 gcaagtttac tgtctttctg ccagtaggag atcagtacat tgctgcccag ccatgtctca
    13141 ccgacaatgt tgaatgaagg atattcatca ttgaccgctt tgcaccaatg cgccatcatt
    13201 tcaaagtcgg cgtaaggatg tgtatcctgg cggattccgt tgatacccgc atattctatc
    13261 caccagatac tactctgtat caggtaagtg gctacgtgac ggtttcgttg gttgaagtcc
    13321 ggcatagtca gtgtgaacca accgtcaatg gccagctttt tatcataatc ggaagtataa
    13381 gggtccattt gggtggcagt tttgaaactg gtctgcatgt agtttccctc aaagttgaac
    13441 caatctttgg caggcatgtc tttgaataga taattgttcg aaccgcaatg gttgaagatc
    13501 atgtccatga ctacctttag ccctttagca ttggcttctt ttacgagatt gcgaaactct
    13561 tcattgcttc cgaggcgacg gtctacttga tagtaatcgg tgatggcata tccatgataa
    13621 gagccttcct tcatgtcatt ttcctgaatc gggttaagcc atatactcgt aactccgagg
    13681 tctgcaatat aatccaaatg tttctctatt ccttttaaat caccgccatg acgggcgaaa
    13741 ggttcgttgc ggtctatgtt tgcttccaac attccgggaa ttatatcatt ggaggggttg
    13801 ccgttggcaa agcggtccgg cataatgaga taaagtacat cactggagtt gaagccttct
    13861 actgctgaag cattctcttt gcgctgttta agttcgtatg gtattttagt ctgtttttta
    13921 ccttgtttca ggagaatgtc gaagtgctga gggatagctt ttgaaagatc taaatataag
    13981 atcagataat taggattttc ctgtttcacc acgtcctgca gggtgatgtc attagaagaa
    14041 atacttacct ctgcggatga tatgccatcg ccgtatagaa gtatttgtag ttcgggattc
    14101 ttcattcctg cccaccaaaa ggtaggagcc actttcttta tattggttgc ggcgaatgct
    14161 tgatgataac ccgatagaag taatagtaaa atagcaaata acaaatttcg tttcat
//
