LOCUS       AF228498               11526 bp    DNA     linear   BCT 03-AUG-2000
DEFINITION  Escherichia coli AgaR (agaR), KbaZ (kbaZ), AgaV (agaV), AgaW (agaW),
            AgaE (agaE), AgaF (agaF), AgaA (agaA), AgaS (agaS), KbaY (kbaY),
            AgaB (agaB), AgaC (agaC), AgaD (agaD), and AgaI (agaI) genes,
            complete cds.
ACCESSION   AF228498
VERSION     AF228498.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Brinkkotter,A., Kloss,H., Alpert,C. and Lengeler,J.W.
  TITLE     Pathways for the utilization of N-acetyl-galactosamine and
            galactosamine in Escherichia coli
  JOURNAL   Mol. Microbiol. 37 (1), 125-135 (2000)
   PUBMED   10931310
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Brinkkoetter,A., Kloess,H. and Lengeler,J.W.
  TITLE     DNA sequence of the aga/kba regulon from Escherichia coli C
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11526)
  AUTHORS   Brinkkoetter,A., Kloess,H. and Lengeler,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2000) Genetics, Univ. of Osnabrueck, Barabarstr.
            11, Osnabrueck 49076, Germany
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C"
                     /db_xref="taxon:562"
                     /map="70.5 min"
     gene            complement(34..843)
                     /gene="agaR"
     CDS             complement(34..843)
                     /gene="agaR"
                     /note="aga regulon transcriptional repressor"
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_DeoR,DBD-SUPERFAMILY|0043758"
                     /protein_id="AAF81081.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVST
                     VTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVELI
                     QPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLRRQS
                     QSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERIIVVTD
                     SSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1092..2372
                     /gene="kbaZ"
     CDS             1092..2372
                     /gene="kbaZ"
                     /note="ancillary protein for ketose bisphosphate aldolase
                     KbaY"
                     /codon_start=1
                     /transl_table=11
                     /product="KbaZ"
                     /protein_id="AAF81082.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPLL
                     IEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPAAQ
                     AMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCLEHF
                     GEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWPRIIA
                     LVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLVIDHFA
                     ILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSHYHGDGN
                     ARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQYVKVRSGE
                     LQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2359..2868
                     /gene="agaV"
     CDS             2359..2868
                     /gene="agaV"
                     /note="pts dependent N-acetyl-galactosamine-IIB component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9KIQ4|4.A.6.1.4"
                     /protein_id="AAF81083.1"
                     /translation="MKANKQNKEEHAMPNIVLSRIDERLIHGQVGVQWVGFAGANLVLV
                     ANDEVAEDPVQQNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTL
                     VKGGVPVNRINVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKTAGVECFVQGVPTEPAV
                     DLFKLL"
     gene            2879..3658
                     /gene="agaW"
     CDS             2879..3658
                     /gene="agaW"
                     /note="pts dependent N-acetyl-galactosamine-IIC component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9KIQ3|4.A.6.1.4"
                     /protein_id="AAF81084.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLGD
                     LHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPFAV
                     AVQMGITFLFSVMSGVMSRCARMPRTPILAALNACNYLALLALGNFYFLCAFLPIYFGA
                     EHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAWLKLP
                     VLAIACPALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            3648..4526
                     /gene="agaE"
     CDS             3648..4526
                     /gene="agaE"
                     /note="pts dependent N-acetyl-galactosamine-IID component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9KIQ2|4.A.6.1.4"
                     /protein_id="AAF81085.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRVA
                     WRSMLLQASFNYERMQASGWLYGLLPALKKINTNKRDLARAMKGHMGFFNTNPFLVTFV
                     IGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLRYCRLCGGIEPVLALQGSILG
                     AVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGALVAT
                     YVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLIAVTVV
                     LGIVGKFCHFL"
     gene            4544..4978
                     /gene="agaF"
     CDS             4544..4978
                     /gene="agaF"
                     /note="pts dependent N-acetyl-galactosamine- and
                     galactosamine IIA component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8Y8V8|4.A.6.1.16"
                     /protein_id="AAF81086.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDVPETSSTALLT
                     SQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVLER
                     EGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            4954..6108
                     /gene="agaA"
     CDS             4954..6108
                     /gene="agaA"
                     /note="N-acetyl-galactosamine-6-phosphate deacetylase"
                     /codon_start=1
                     /transl_table=11
                     /product="CE9"
                     /protein_id="AAF81087.1"
                     /translation="MSGRGRNMTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPAG
                     VTERDAELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTTPLN
                     TIHAALKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAV
                     SQHTLRVVALAPEKEGALQAIRHLKQQNVKVMLGHRAATWQQTRAAFDACADRLVHCYN
                     RMTGLHHREPGIVCAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAA
                     GMPDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASL
                     HPARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6457..7611
                     /gene="agaS"
     CDS             6457..7611
                     /gene="agaS"
                     /note="unknown function"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|SIS,STP|SIS"
                     /protein_id="AAF81088.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNFL
                     EPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPAHP
                     LLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALLMPA
                     ETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTTLGDFSEGVFGYA
                     PWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDETLVVVF
                     VSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFIDVEQAF
                     CFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7624..8484
                     /gene="kbaY"
     CDS             7624..8484
                     /gene="kbaY"
                     /note="tagatose-1,6-bisphosphate aldolase"
                     /codon_start=1
                     /transl_table=11
                     /product="KbaY"
                     /protein_id="AAF81089.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSPV
                     ILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAMID
                     GSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEAKRF
                     VELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFVRRTI
                     ELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRNYMRVGMDAMKEVVRNKINVCGSA
                     NRISA"
     gene            8651..9127
                     /gene="agaB"
     CDS             8651..9127
                     /gene="agaB"
                     /note="pts dependent galactosamine IIB component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P42909|4.A.6.1.5"
                     /protein_id="AAF81090.1"
                     /translation="MTSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDI
                     QQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKD
                     VNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            9166..9969
                     /gene="agaC"
     CDS             9166..9969
                     /gene="agaC"
                     /note="pts dependent galactosamine IIC component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9KIP7|4.A.6.1.5"
                     /protein_id="AAF81091.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAILG
                     DIQTGLITGGLTELAFAGLTPAGGVQAPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFS
                     LLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLA
                     QGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVCYIQV
                     SNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            9959..10750
                     /gene="agaD"
     CDS             9959..10750
                     /gene="agaD"
                     /note="pts dependent galactosamine IID component"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P42911|4.A.6.1.5"
                     /protein_id="AAF81092.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKKI
                     YKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPIAG
                     IGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGVKAI
                     DKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDNTHSVALQQDFFDKVFPN
                     ILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            10751..11506
                     /gene="agaI"
     CDS             10751..11506
                     /gene="agaI"
                     /note="galactosamine-6-phosphate isomerase (deaminase)"
                     /codon_start=1
                     /transl_table=11
                     /product="AgaI"
                     /protein_id="AAF81093.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRS
                     KPNAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETFLQ
                     QHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLNEPG
                     ESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGKQDAT
                     DRFLTAKVSTAIPASFLWLHSNFICLINT"
ORIGIN
        1 aacgcattcg tgttgaccag cgggcatcct ttctcactcc ccgaccagaa tcacttcaac
       61 cccagccttt cgcagtcctt ccaggctatc cgcaggaatg ccttcatcaa caatgatcat
      121 gtcgatacgt tgagtatcaa tgatcttatg taaactggaa cgattgaact tactggaatc
      181 ggtgaccacg atgatccgtt ccgcaacttc gcacatccga cggtttaaac gagcttcatc
      241 ttcattatgt gtgctgacgc cgcgctccag atcgatcgca tctacaccaa gaaacagcat
      301 atcgaagtgg taattttgca gcgattgctc agcctgatcg ccgtaaaaag attgcgactg
      361 acggcgcaaa tgcccgccgg tcatcagcag ctcaacgcct tccgcttcca gcaacgcatt
      421 agccacgttc ataccgttgg tcatcgcaat tacgtcagtg tgcttgcgca tcagacgagc
      481 aatctcaaaa gtggtggtcc cggaatcgag gataacccga tgacctggct gaatcaactc
      541 aacggcagct ttcgcaacgc tgcgtttcat cgcggtgttc agtgcgcttt tatcttccac
      601 tgatggctcg actgacggcg tcgtgctatc gcagatcaac gcgccaccat aggcacgcac
      661 agcgatcccc tgcttttcca gaaacgccag atcgttgcgg atcgtcacag tagatacgcc
      721 atacaatgcc gacagatcgt taacctgcac actcccttgc tgtcgcagac gctgaatgat
      781 ctgttctcgt cgctcgctgg tgccagtcac tcgcttctca cctgaagcgt cggtattact
      841 catagtaagt cctttcgtaa aactttcgtt tcatttcgtt ttgcctatta acgcctttct
      901 attaagcaaa tgcaagccca ccttgcccat tgacgcaagc tactctcgtt tcagtgactt
      961 tcattatgtt tcttttgtga atcagatcag aaaactatta tctttcgttt tatttttatc
     1021 tcaccatgac gcagtatcaa ctgaaacaaa acgaaagatt aatatcgcag taatctgaac
     1081 tggagaggaa agtgaaacat ctgacagaaa tggtgagaca gcacaaagcg ggcaaaacaa
     1141 atggaattta tgccgtttgt tccgcacatc cgctggtgct ggaagctgca atccgctacg
     1201 ccagtgcaaa ccaaacgccg ttactgattg aagcaacctc caatcaggta gaccagttcg
     1261 gcggttatac cggaatgacg cccgccgatt ttcgcggctt tgtttgtcag ctcgccgact
     1321 cgttgaattt cccgcaggat gcgttgattc tgggtggtga ccatctgggg ccaaaccgct
     1381 ggcaaaacct gccggccgct caggcaatgg ccaatgctga tgatttgatt aaaagctacg
     1441 ttgcggcagg attcaaaaaa atccaccttg attgcagcat gtcctgtcag gacgatccga
     1501 ttcccttaac tgatgacatc gtggctgaac gcgccgcccg tctggcgaaa gtggcggaag
     1561 aaacctgtct tgaacacttt ggcgaagccg atctggagta tgtcattggt accgaagtgc
     1621 cggtacctgg cggcgcgcat gaaaccttaa gcgagctggc ggtcaccacg ccggatgccg
     1681 cccgcgccac gctggaagcc catcgtcacg cctttgaaaa gcaaggtttg aatgccatct
     1741 ggccacgcat cattgccctg gtggttcaac ccggcgtcga gttcgatcac accaacgtta
     1801 ttgattatca gcccgccaaa gcgagcgcct taagccagat ggtcgaaaac tacgaaacgc
     1861 tgattttcga agcgcactct accgattatc aaacgccgca atcgctgcgc cagctggtga
     1921 ttgaccactt tgccattctg aaagttggcc cagcgctgac cttcgccctg cgtgaagctc
     1981 tgttctctct ggcggcgatt gaagaagaac tggtgccagc gaaagcctgt tctggtctgc
     2041 gtcaggtgct ggaagacgtg atgctcgacc gcccggaata ctggcaaagc cactaccacg
     2101 gtgacggcaa cgcgcgtcgt ctggcgcgtg gttatagcta ctcggatcgc gtgcgctatt
     2161 actggccgga cagccagatt gatgacgctt tcgctcatct ggtacgtaat ctggcggatt
     2221 caccaattcc gctgccgctg atcagccagt atctgccgct gcagtacgtg aaagttcgct
     2281 ccggcgagct gcagccaacg ccacgggaac tcattatcaa ccatattcag gacatcctgg
     2341 cgcagtacca cacagcctgt gaaggccaat aagcaaaaca aagaggaaca cgctatgcca
     2401 aatattgttt taagccggat tgatgaacgc ttgattcacg gtcaggtcgg cgttcaatgg
     2461 gtcggatttg cgggggcaaa tctggtgctg gtagccaacg atgaggttgc cgaagatccg
     2521 gtacaacaaa acctgatgga aatggtactg gcagaaggga tcgccgtacg tttctggacg
     2581 ctgcaaaaag ttatcgacaa cattcatcgc gccgccgatc gacagaaaat cctgctggtt
     2641 tgtaaaacac ccgccgattt cctgacgctg gtgaaaggtg gcgttccggt gaatcgcatt
     2701 aacgttggca atatgcacta cgccaatggc aaacaacaaa tcgccaaaac ggtttctgtg
     2761 gatgcgggcg atatcgcagc atttaacgac ctgaaaaccg ctggggtgga atgcttcgtt
     2821 cagggcgtcc cgacagagcc tgctgtggac ctctttaaat tactttgagg gattcatcat
     2881 ggaaatcagc ctgttgcagg catttgcgtt gggcattatc gcctttatcg ctggcctgga
     2941 tatgtttaac ggcctaaccc atatgcaccg cccggtggtc ctcggcccgt tggtcgggct
     3001 ggtacttggc gatctgcata ccggaatttt aaccggcggt acgctggaac tggtgtggat
     3061 ggggctggcc ccgctggcgg gcgcacagcc gcctaacgtg attatcggta ctatcgtcgg
     3121 cacggcgttt gccattacta ctggcgtgaa acccgatgtc gcagtaggtg tcgccgtacc
     3181 tttcgctgtc gcagtacaga tggggattac cttcctgttc tcggtgatgt ccggcgtgat
     3241 gtctcgctgc gcccggatgc cgagaacgcc gatactcgcg gcattgaacg cgtgtaacta
     3301 tctggcactg ctggcactcg gcaatttcta ttttctctgc gctttcctgc ctatctactt
     3361 cggggcggaa catgcaaaaa ccatcattga tgtcctgccg caacgattaa tcgacggcct
     3421 cggcgttgcg ggcggcatca tgccagcaat cggttttgcc gtgctgctga aaatcatgat
     3481 gaaaaacgtc tacatcccct acttcatcct gggctttgtt gccgccgcct ggctcaagtt
     3541 accagtgctg gctattgcat gccctgcgct tgcgatggcg cttatcgact tgctgcgtaa
     3601 atctcctgaa ccgactcaac ctgcggcaca gaaagaggaa tttgaagatg gcatctaatc
     3661 aaaccaccct gccgaacgtc agtgaaaacg aagaaacatt gctgactggc gtcaatgaaa
     3721 acgtgtatga agatcagagc attggcgcgg agctgacgaa aaaagatatc aaccgcgtcg
     3781 catggcgttc catgctgtta caagcgtctt ttaactacga acgtatgcag gcttctggct
     3841 ggttgtacgg tctgctgcct gctctgaaaa agatcaacac taataaacgc gacttggcgc
     3901 gcgcaatgaa ggggcatatg ggtttcttca ataccaatcc gtttctggtg acgtttgtta
     3961 tcggcattat ccttgcgatg gagcgttcta agcaggacgt taacagtatt cagagcacca
     4021 aaattgccgt cggtgcgccg cttggcggaa ttggcgatgc aatgttctgg ttacgctact
     4081 gccgattgtg cggggggatt gagccagtct tggcattgca aggctccatt cttggcgcag
     4141 tcgtctttat tgtgctgttc aacgtggtac acctggggct gcgttttggt ctggcgcatt
     4201 atgcttaccg catgggcgtg gcggcgattc cactgattaa agcgaatacc aaaaaagtcg
     4261 gccatgctgc gtctatcgtt gggatgacgg taatcggtgc gctggtggca acctatgttc
     4321 gtttaagcac cacgctggaa atcacggcgg gcgacgcagt ggttaagtta caggctgatg
     4381 ttatcgacaa actgatgccg gccttcttac cgttggtcta caccctgacc atgttttggt
     4441 tggtacgccg cggctggagt ccgctgcgtc tgattgcagt taccgtggtt ctcggcatcg
     4501 tcggtaaatt ctgccatttc ctttaaatac aatgaggtat tagatgttaa gtattatttt
     4561 gacagggcat ggcgggtttg ccagcggcat ggaaaaagca atgaagcaaa ttctcggtga
     4621 gcaatcgcag tttatcgcca tcgacgttcc ggaaacctca agcaccgcgc tgcttacatc
     4681 gcagcttgaa gaggccatcg cccaactgga ttgtgaagac ggcattgttt ttctgacgga
     4741 tttgctgggc ggcacgccgt ttcgcgtagc ttcaacgctg gcgatgcaaa aaccgggctg
     4801 tgaagtgatc accggcacca atttgcaact gctactggag atggtgctgg agcgcgaagg
     4861 gttaagcggt gaagaattcc gtgtacaggc gctggagtgt ggacaccgag ggctgaccag
     4921 tctggtggat gagttaggcc gctgccatga agaatgtccg gtcgaggaag gaatatgaca
     4981 cacgttctgc gcgccagaag gctgctgact gaagagggat ggctcgatga ccatcagttg
     5041 cgtattgctg acggtgtcat cgcagcaatc gaaccgattc cagcgggcgt gactgaacgc
     5101 gatgcggaac tgctctgccc tgcttacatc gacacccatg tacacggtgg tgcgggcgtt
     5161 gatgtaatgg atgacgcgcc ggatgtactc gacaagctgg caatgcacaa ggcacgcgaa
     5221 ggtgtcggca gttggttacc gaccaccgta accacgccgc ttaataccat tcatgcggcg
     5281 ctgaaacgta ttgctcaacg ttgccaacgc ggcggacctg gtgcgcaagt gctggggagt
     5341 tatctcgaag gaccgtactt cacgccgcag aataaaggcg cgcatccgcc ggagttgttc
     5401 cgcgagcttg aaattgccga gctggatcag ttgattgccg tttctcagca caccttacgt
     5461 gtggtagcgc tggcaccgga aaaagaaggg gcattgcagg ccatccgcca tcttaaacag
     5521 caaaacgtaa aggtgatgct ggggcatagg gcggcgacct ggcaacaaac ccgcgccgca
     5581 tttgatgctt gtgccgaccg ccttgtgcat tgctataacc ggatgacagg tttacatcac
     5641 cgcgaaccgg gaattgtttg cgcgggatta acggacaagc gcgcctggct ggaactgata
     5701 gccgatggtc atcatgtgca tccggcggca atgtcgctgt gttgttgctg tgcgaaagag
     5761 agaatcgtac tgatcaccga cgcgatgcag gcagctggga tgccggatgg tcgctatacg
     5821 ttatgtggtg aagaagtgca gatgcacggt ggcgttgtcc gtaccgcgtc tggtgggctg
     5881 gcgggcagta cgctgtctgt tgatgcggca gtgcgcaata tggtcgagtt gacgggcgta
     5941 acgcctgcgg aggccatcca tatggcgtcg ctgcatccgg cgcgaatgct gggtgttgat
     6001 ggtgttctgg gatcgcttaa accgggcaaa cgcgccagcg tcgttgcgct ggatagcggg
     6061 ctacatgtgc aacaaatctg gattcagggt caattagctt cgttttgata gtttgctcct
     6121 ttattgggcc ttcacttccc ccgtaaggcc tttctttttc tttcgttttg atctgtgcag
     6181 cggtgtcgga tgcgastaac gcgtcttatc cgacctacag ttggtgaccg caaggccgga
     6241 ttaagcgttt gcgccgcatc cagcaatccc ttttgcttcc tttatctttt ctttcaacga
     6301 tcacaaattt cgttttattt cttttttctc cattgaactt tcagtttctt ttctatagat
     6361 tttaatcaac gaaagacatc accaagtgaa atgaaacgaa aggcaagtga aagcgacaac
     6421 gcccgacgtc aagttcatca gactaaggat tgagttatgc cagaaaatta cacccctgct
     6481 gctgccgcaa ccggtacatg gactgaagaa gagatccgcc atcagcctcg cgcatggatc
     6541 cgttcactca ccaacatcga cgcgctacgt tccgcgctca ataacttcct tgaaccgtta
     6601 ctgcgcaaag agaatctgcg gatcatcctg accggagccg gaacgtcggc atttatcggt
     6661 gacatcatcg cgccgtggct cgccagccat accggtaaaa acttcagcgc cgtaccgacc
     6721 accgatctgg tcaccaatcc gatggactac ctgaacccag ctcatccgct gctgttgatc
     6781 tccttcggtc gatccggcaa cagcccggaa agcgtcgcag ccgtggaact ggcaaatcaa
     6841 tttgtaccgg aatgctatca cctgccgatc acctgcaacg aagcgggcgc tctttaccaa
     6901 aacgcgatca acagcgataa cgcgtttgcc ctgctgatgc ccgcagaaac gcacgatcgc
     6961 ggctttgcga tgaccagcag cattaccacc atgatggcca gctgcctcgc ggttttcgca
     7021 cctgagacga tcaacagcca aaccttccgc gacgtggcgg atcgttgcca ggcgatcctg
     7081 accacactgg gcgatttcag cgaaggtgtg tttggttacg caccgtggaa acggatcgtt
     7141 tatctcggta gcggtggctt acagggcgca gcacgcgagt cggcgctgaa agtgctggaa
     7201 ctgacggcgg gtaaactggc ggccttttat gattctccaa ccggattccg tcatggacca
     7261 aaatcgctgg tcgatgacga aacgctggtg gtggtatttg tctccagcca cccttacacc
     7321 cgtcagtatg atcttgatct gctggctgaa cttcgccgtg acaaccaggc aatgcgtgta
     7381 atcgccatcg ccgcggaaag cagcgacatc gtcgctgccg gtccacatat catcctgcca
     7441 ccgtcacgtc actttatcga cgttgagcag gcattttgct tcctgatgta cgcccagacg
     7501 tttgcactga tgcagtcgct gcacatgggc aatacgccgg ataccccatc agccagtggc
     7561 accgttaacc gcgtggtgca aggcgtaatc attcatccgt ggcaggcata agaggatcgc
     7621 attatgagca ttatctccac taaatatctg ttacaggacg cccaggccaa tggctacgcg
     7681 gtgcctgctt ttaacattca taacgccgag acgatccaag cgatcctcga agtgtgcagt
     7741 gaaatgcgat cgccggtgat cctcgccgga acgccgggga cctttaaaca catcgcgctg
     7801 gaagagatct acgccctgtg tagcgcctat tccacaacct acaacatgcc actggcgctg
     7861 catctcgacc accacgaatc gctggatgat attcgccgta aagtccacgc aggtgtgcgc
     7921 agtgcgatga tcgacggcag ccacttcccg tttgccgaga acgtgaagct ggtgaaatcg
     7981 gttgttgact tctgccactc acaagattgc agcgtggaag cagaactggg ccgcctgggc
     8041 ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat tcctgaccga tccacaagaa
     8101 gctaaacgct ttgtcgaact gactggcgtc gacagcctgg cggtagcgat tggtacggcg
     8161 cacggcttat acagcaaaac gccgaagatt gatttccagc ggctggcgga aattcgtgaa
     8221 gtggtggatg ttcctctggt gctgcatggt gccagcgatg ttccggatga atttgtccgt
     8281 cgcactattg aacttggcgt cacaaaagtg aacgttgcca cagaattaaa aatagccttc
     8341 gctggcgcgg ttaaagcctg gtttgcggaa aatccgcagg gtaatgatcc tcgtaattat
     8401 atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa ataaaattaa tgtctgtggt
     8461 tcagcgaatc gaatttcagc ataatcatta gttttttatg atttatccca atgtacttcc
     8521 cgattaatat cggggagtgc cttaatggaa aaggagataa ctaaacctta ataaatacat
     8581 cactacaata tcgcaacaat aatatattta aaaaaattat attattcaac tttatggtga
     8641 ggattacaca atgaccagtc caaatattct cttaacccgt attgataacc gtctggttca
     8701 cggtcaggtt ggcgtgacct ggacatccac catcggtgca aatctgctgg tagtcgtgga
     8761 tgatgttgtc gctaacgatg atattcaaca gaaattgatg ggtattaccg cggaaaccta
     8821 cggctttggc attcgtttct ttactatcga aaaaaccatt aacgtcatcg gcaaagctgc
     8881 accacatcag aagatcttcc tgatttgccg tacgccacaa acggtacgta aattggtaga
     8941 aggtggtatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg aagggaaaaa
     9001 gcaaatcagc agtaaagttt atgtcgatga ccaggatctc acggacttac gttttattaa
     9061 acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag aacaaatccc
     9121 tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca tgaaataacc
     9181 ctacttcagg gattatccct ggcggcgtta gtttttgttc tggggattga tttttggctg
     9241 gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc tattctcggt
     9301 gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc cggattaacc
     9361 cctgcaggtg gtgttcaggc gcccaacccg attatggcgg gtctgatgac caccgtcatt
     9421 gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc gtttagtttg
     9481 ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat gaccaaagcc
     9541 gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg gacaacgatg
     9601 ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct ggcacagggg
     9661 gcgatgcagg cgctggtgaa agcgatgccc gcctggctga cccacggctt tgaagtggct
     9721 ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt caaagcgcaa
     9781 tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccaggt cagcaacctg
     9841 ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa tgcgaaatcc
     9901 cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga ctacagcaat
     9961 gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt cgctgctgca
    10021 agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga tgttgccgat
    10081 cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga aagataacct
    10141 cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat taatttcgat
    10201 ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac tgtttggccc
    10261 aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta tggcgggaat
    10321 ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt tcgccgttta
    10381 cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag tcggcgtgaa
    10441 ggcgatcgat aaagtgcgag agaactcgca gatgattgcc cgttcggcaa ccatcctcgg
    10501 gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg tgacatcgtt
    10561 tgccatcgac aatacccaca gcgttgcgct gcagcaggat ttcttcgata aagtcttccc
    10621 gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg tgaaaaaagc
    10681 gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt ccgcattcgg
    10741 cattttgtaa atggaacgag gcactgcgtc tggtggtgcc tctttactta aggaatttca
    10801 tcctgtgcaa acccttcagc aagttgaaaa ctatacggcc ttaagtgaac gtgccagcga
    10861 atatttattg gccgtgatcc gtagcaaacc gaatgccgtg atttgcctgg cgaccggagc
    10921 cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc aggttgatgt
    10981 cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa cgatgccagg
    11041 cacctgcgaa actttcctgc aacagcatat cgtgcagccg ctggggctac gtgaagacca
    11101 gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg taacgaacct
    11161 gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa acggtcatct
    11221 tgggctgaac gaaccgggag aaagcctgca accggcctgc catatcagtc aacttgatgc
    11281 cagaacacag caacatgaga tgttaaaaac cgcgggtcgc cccgtgactc gtgggatcac
    11341 cttaggcctg aaggatattc tcaatgcccg cgaagttttg ttactggtga ctggcgaagg
    11401 aaagcaggat gcgacagatc gttttctcac ggctaaagtc tctaccgcta tcccggcttc
    11461 atttttatgg ctgcacagta actttatttg tttaattaac acctaaccag atgaaaaatt
    11521 tgctca
//
