CAZyme Information: MGYG000004816_01283

Basic Information

• Species: Phascolarctobacterium_A sp900757795
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Acidaminococcales; Acidaminococcaceae; Phascolarctobacterium_A; Phascolarctobacterium_A sp900757795
• CAZyme ID: MGYG000004816_01283
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
519	MGYG000004816_13|CGC1	59259.56	9.0571

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004816	1941300	MAG	China	Asia

• Gene Location: Start: 9202; End: 10761 Strand: +

Full Sequence      

MKQLEKNSLLIIGGIALFTILFGIGGVPLLDPDEPVYAETAREMLQTGDFLSPRIFGSYW
YDKPPMYYWLVAISQMLFGFSEFSARLPAALMACGTAIMVYVSTTKMFNEKAGFWSALVL
TSCIQFFYMGKAAVTDTTLLFFMTGAILSFAHKKYWLMYACMALATVTKGPIGIVFPGAI
IFLYLAITGQLREILRMHVIRGLLLYFLIASPWYYAMYTVHGMEFINTFLGFHNITRFTT
PEHANRVTFWYYFPVVILGMFPWTGMLVQAIKASICESRIDDMRQLLLYHVWWIFVFLFF
TVCQTKLVSYILPMFPPLAVIIGWNISRMLAKLRHNTTFYSWMFGSGLLFLLLGAGWIIG
SQYLPETAFSLIMLGVLTVILGFGAVFSLWYYRDIEMSCWLHTLIGVVTMCVAFAFVMPV
LADRFSVKTMSGVYLEQCEQATPIYVDKFLRPGFMYYAGKPGIEMLPKTGALSEALRNGQ
HKYILVRGLEYRRVQKTKQIPTNVTTVKEISDIYLLEQK

Enzyme Prediction     

No EC number prediction in MGYG000004816_01283.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	3	506	1.6e-118	0.9574074074074074

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1807	ArnT	1.85e-63	15	393	16	401	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
PRK13279	arnT	2.90e-41	8	334	8	341	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
pfam13231	PMT_2	2.36e-18	62	215	1	159	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	1.91e-08	37	226	35	245	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
COG4346	COG4346	0.009	64	332	137	415	Predicted membrane-bound dolichyl-phosphate-mannose-protein mannosyltransferase [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QTV77242.1	0.0	1	518	1	518
QNH53437.1	1.22e-167	2	494	5	502
AOH48432.1	9.87e-167	2	496	5	504
ANR70824.1	1.98e-166	2	496	5	504
AME03144.1	2.39e-162	2	493	5	501

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	3.32e-37	29	329	54	367	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O67601	9.11e-45	6	353	2	327	Uncharacterized protein aq_1704 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1704 PE=3 SV=1
O67270	1.47e-37	3	342	1	338	Uncharacterized protein aq_1220 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1220 PE=3 SV=1
Q6D2F3	5.67e-37	15	372	14	387	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=arnT PE=3 SV=1
A0KGY4	6.99e-37	17	332	13	336	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / BCRC 13018 / CCUG 14551 / JCM 1027 / KCTC 2358 / NCIMB 9240 / NCTC 8049) OX=380703 GN=arnT PE=3 SV=1
C6DAW3	9.31e-36	15	372	14	387	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) OX=561230 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999986	0.000023	0.000003	0.000000	0.000000	0.000010

TMHMM  Annotations     

start	end
7	29
83	105
126	148
163	185
198	220
248	265
286	303
307	326
338	360
370	392
399	421