CAZyme Information: MGYG000004517_00202

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; UBA5884
• CAZyme ID: MGYG000004517_00202
• CAZy Family: GT2
• CAZyme Description: D-alanine--poly(phosphoribitol) ligase subunit 1

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2562	MGYG000004517_2|CGC1	290607.31	5.0636

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004517	2174745	MAG	Israel	Asia

• Gene Location: Start: 18897; End: 26585 Strand: -

Full Sequence      

MSENKDLMKKALLEIQRLKKLLSEKTISNEPTAIIGMACRLPNGIKNTQDFWQSLESGQD
DIINVPENRWNEYDKDELLKNPYLQKAGFLTEDIEEFDSRLFKIPPKEASYIDPQQRMVL
KVCWEVLENAGYSPTELKGKKIGVFCGVAQTDFLNENITRNEHDIDVTGMNVSFISGRIS
YFFGFHGPALTIDTACSASASAINQAIKSLRDNDCEMAIVCGVNIMFSPETTKKLSALNI
LSKTCELKSFDKFANGTVRGEGCCAILLKNLSKATADNDSIHCVIKGSYINHDGASTSLT
APNGFAQEELLKSAWIKSGITPNDIDYIETHGTGTALGDPIEIKSISNVIGSNRTEPLYI
GSLKASIGHLEPASGIASVIKTALMLEHKKIVKSINYNVPSEYVNWNAISVKVAQNLMDW
NKSNGKNRVAGVSSFGLSGTNVHIVLEEYNKDTVTTQDMPVYPFMFSAVTENALIEQMKK
FLAYVETCKDINLTNLSYTQNVTRAILEVRTLIMAKNLDDLKSLLNKAISGQMDSNIFTQ
KGLSKKKIVFTFTGQGSQYNNMCKDFYKNPYFKQAFDMCDKYYNQLTGGSLIDLVFSEKY
DLSQTVYTQPAIFSVEYSLAYMYQKYGINPSIVFGHSIGEYVSACISGVFSLEDAMKLVV
TRGKAIQEKAIFGKMMAIFTDKECIKDIIKPYNDVYISLSNSQQQTVIAGSEKSILEIDG
ILNAKDIKHTILNTTRPFHTPFMQDVSNDFFEVAKTVTYSKPKLNIISNVTSKAETTLFT
TADYWKQHIVSEVRFLDSVLNLGNLSEYLFLEVGAMPILSGLIGRISNGNADCIYSASKD
TSADYKIAEILSSMFLYAIPFNMRGYYKSFNAQTCDIPNYAFDTKKIPYIHNLYEKVSYT
TPVINEVEETSNSSLELTDEKISEYIVNLLVYHLGIDKSDIDENTNLLTLGLSSLNGVKI
VGDIKKHFNVEINLNDFFNNCTVSGWKELLKGLTVSNSADKQVNQVTIDEQHRYDMFDLN
DIQYAYWAGRDNKNMLLSNNACCTYFELDMPNLDIEKFKHTLKCLEQRHDMLRCRMNKDA
KQYIVKDEYSTVVVYDYKSISDMQKHLDNIRTTMSYEILPIDKPMFKVAITKLDDINYRI
HFSIDFMIADAMSLYIFWKDMGKLYNGEILEPLTITYKDYLNYLNNSSDIKAKHDVDKKY
WLSKIEDFPKSPELPFKSSEFVDNKHKFVRRRKNINPEQWKKFTQNCAKYGLTPSSALFT
LYAEVLSAFGGGSSFAIMMTVFNRENINKDVQKIIGDFTKLALIDVHRKDTTVSKNALDI
QSTIMNSISHTEYSATEFVGELRKHFNEDRMYTVVFTSAIGIDDLNKDISDNDAIFLKSS
NSLISSTPQVCIDHQIFYEDKDIVLSWDTLDGVFMDNVVSAMFDVYTSLVDKAIENPEFF
NQTLIDLRPSYQVEVHDKANMTTIEYTPVTLLTGFNKNVLENPNNVAVVTNGNSYTYRQL
DECSNKVANQLIQDGVTIGDRVLVELPKSFEQIYSIVGILKAGAVYVPITYKQPKNRTIN
IIEKSIPKAIIGDNKFNDMGITYYSIIDFESSSSDGTNLPTISASDGAYIIYTSGSTGNP
KGVYIAHGSAMNTIDDVTRKYNITSNDSTIAISSLSFDLSVYDVFGMLSVGGKIVIPTEQ
ERIDPKSQYQLVKHNDVTVWNTVPAIMDLYLDFLNKKSLTSESIRKVILSGDWIPLNLLD
KLYKALPNAELTSMGGATEASIWSNYFNVDTLNPEWNSIPYGYPLANQRFYILDDFNRRC
PDYVSGKLHIAGDGLAECYYNEPSLTENAFYVHKSIGERLYNTGDYGKYISDGVIEFLGR
KDGQIKINGYRIEIGEVISAIKKCGIDSKAIIMPVGNSRKKIVAFLIQKDTIDQELLKQE
LGKYLPKYFIPDKIICIEDLPVTANGKSDMKKLYQIYEDIKNSSKASNTSNENVNPILKK
IREILDISEITEDDSFSAFGVSSVDMIRLADELESIYGERPSISDMISYKSVSELINFFD
GKVANDNNNFVQQQDNSQLDDDDNFVYTTTELQENPLEKYSNQGIELYLEDGKLKFKASK
GKMTPQLKAELIANKQSLIEYLTVKAEQEKHDAEYFKENSFLLTPLQKAYLLGRSNYYEL
GGTSAHYYTEIEWNNLDLVKLEKSVNKVIRYNDILQTVVFSNGTQAVLENMPYYKVKVSK
VSNEEFLQVRSTWSEHKYEIGKWPMFDIIVSDLGNDNYIVHFSFDCILLDGWSANMMISE
IFEVYEGRTIKKPQLSFKDYVTNESIFLEDKEYHKKAIEYWENSVQSLPNAPELKYKVDF
SQVETPHFKRKRFVLDKNKTHLLNEKIKKYGLTASAVICTAYMSVLSKYSNTHDFTLNLT
LFNRLPLHNDVWNILGDFTNITLIPYFESKNSTFMDSLTSTKNYLVEAIEHRTYNGLELL
KHFSDDNILKAVMPVVFTSMIFGNLESSSNENDIFDSVKEVYSISQTPQVSLDHQALVRN
NELVLIWDYVSELFDAEIIDNMFNDYINFINTIANSDNWEIL

Enzyme Prediction     

No EC number prediction in MGYG000004517_00202.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3321	PksD	0.0	28	887	2	870	Acyl transferase domain in polyketide synthase (PKS) enzymes [Secondary metabolites biosynthesis, transport and catabolism].
cd00833	PKS	2.69e-177	30	446	1	421	polyketide synthases (PKSs) polymerize simple fatty acids into a large variety of different products, called polyketides, by successive decarboxylating Claisen condensations. PKSs can be divided into 2 groups, modular type I PKSs consisting of one or more large multifunctional proteins and iterative type II PKSs, complexes of several monofunctional subunits.
cd12114	A_NRPS_TlmIV_like	1.26e-170	1483	1953	1	477	The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
cd05930	A_NRPS	2.49e-134	1483	1953	1	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
smart00825	PKS_KS	5.11e-128	33	448	2	298	Beta-ketoacyl synthase. The structure of beta-ketoacyl synthase is similar to that of the thiolase family and also chalcone synthase. The active site of beta-ketoacyl synthase is located between the N and C-terminal domains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAZ00088.1	9.80e-195	33	2035	1215	3288
BAZ75991.1	9.80e-195	33	2035	1215	3288
BAY90071.1	4.08e-193	33	1953	1214	3196
BAY30132.1	1.74e-191	33	1953	1217	3207
AFY93865.1	1.24e-112	24	982	953	1968

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4MZ0_A	2.20e-181	20	882	29	929	ChainA, CurL [Moorena producens 3L],4MZ0_B Chain B, CurL [Moorena producens 3L]
7LY7_A	1.64e-157	1017	1953	11	932	ChainA, BmdB, Bacillamide NRPS [Thermoactinomyces vulgaris]
7LY4_E	1.68e-157	1017	1953	11	932	ChainE, BmdB, bacillamide NRPS [Thermoactinomyces vulgaris]
7VEE_A	5.75e-138	25	886	16	909	ChainA, Polyketide synthase [Streptomyces graminofaciens],7VEF_A Chain A, Polyketide synthase [Streptomyces graminofaciens]
7S6B_A	4.32e-133	5	820	32	861	ChainA, Polyketide synthase [Streptomyces lasalocidi],7S6B_B Chain B, Polyketide synthase [Streptomyces lasalocidi]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P48633	1.64e-183	930	2561	28	1911	High-molecular-weight protein 2 OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) OX=393305 GN=irp2 PE=3 SV=1
D4AU31	5.44e-133	28	923	623	1545	PKS-NRPS hybrid synthetase swnK OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=swnK PE=3 SV=1
Q1B6A7	9.76e-133	934	1951	19	1031	Phenyloxazoline synthase MbtB OS=Mycobacterium sp. (strain MCS) OX=164756 GN=mbtB PE=3 SV=1
B2HIL7	2.63e-131	8	886	19	912	Phenolphthiocerol synthesis polyketide synthase type I Pks15/1 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=pks15/1 PE=1 SV=1
E9F8M3	3.80e-131	28	882	615	1494	PKS-NRPS hybrid synthetase swnK OS=Metarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) OX=655844 GN=swnK PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000051	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000004517_00202.